The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cells
Hydrogels based on high molecular weight poly(ethylene oxide) (PEO) copolymers of ethylene oxide and propylene oxide, PEO/alginate and PEO/chitosan were synthesized by UV crosslinking of polymer aqueous solutions. These hydrogels were then characterized in terms of their gel fraction yield, degree o...
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| Format: | Article |
| Language: | English |
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Society of Chemists and Technologists of Macedonia
2006-12-01
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| Series: | Macedonian Journal of Chemistry and Chemical Engineering |
| Subjects: | |
| Online Access: | https://mjcce.org.mk/index.php/MJCCE/article/view/294 |
| _version_ | 1818645464685215744 |
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| author | Ruzica Jovanovic-Malinovska Samuel A. Amartey Slobodanka Kuzmanova Eleonora Winkelhausen Maja Cvetkovska Christo Tsvetanov |
| author_facet | Ruzica Jovanovic-Malinovska Samuel A. Amartey Slobodanka Kuzmanova Eleonora Winkelhausen Maja Cvetkovska Christo Tsvetanov |
| author_sort | Ruzica Jovanovic-Malinovska |
| collection | DOAJ |
| description | Hydrogels based on high molecular weight poly(ethylene oxide) (PEO) copolymers of ethylene oxide and propylene oxide, PEO/alginate and PEO/chitosan were synthesized by UV crosslinking of polymer aqueous solutions. These hydrogels were then characterized in terms of their gel fraction yield, degree of equilibrium swelling, shear storage and loss moduli. The physico-mechanical properties of the hydrogels were then correlated to their ability to sustain the viability and the activity of immobilized cells. The production of ethanol by immobilized Saccharomyces cerevisiae was used to test the suitability of the PEO based hydrogels as immobilization matrices. The PEO hydrogel with a value of 255 Pa for Gʹ and 11 Pa for Gʹʹ, showed the best mechanical properties of all the gels tested. Scanning electron microscope (SEM) analysis of the imobilized S. cerevisiae showed proliferation of the yeast cells entrapped inside the polymeric matrix. |
| first_indexed | 2024-12-17T00:31:10Z |
| format | Article |
| id | doaj.art-161f05ecaab74b63834b4a7a20e992b6 |
| institution | Directory Open Access Journal |
| issn | 1857-5552 1857-5625 |
| language | English |
| last_indexed | 2024-12-17T00:31:10Z |
| publishDate | 2006-12-01 |
| publisher | Society of Chemists and Technologists of Macedonia |
| record_format | Article |
| series | Macedonian Journal of Chemistry and Chemical Engineering |
| spelling | doaj.art-161f05ecaab74b63834b4a7a20e992b62022-12-21T22:10:17ZengSociety of Chemists and Technologists of MacedoniaMacedonian Journal of Chemistry and Chemical Engineering1857-55521857-56252006-12-01252113119166The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cellsRuzica Jovanovic-Malinovska0Samuel A. Amartey1Slobodanka Kuzmanova2Eleonora Winkelhausen3Maja Cvetkovska4Christo Tsvetanov5Faculty of Technology and Metallurgy, Ss. Cyril & Methodius University, Skopje,Division of Biology, Imperial College of Science Technology and Medicine, London, United Kingdom 3Institute of Polymers, Bulgarian Academy of Sciences, Bl. 103–A, Acad. G. Bonchev Str., 1113 Sofia,Faculty of Technology and Metallurgy, Ss. Cyril & Methodius University, Skopje,Faculty of Technology and Metallurgy, Ss. Cyril & Methodius University, Skopje,Faculty of Technology and Metallurgy, Ss. Cyril & Methodius University, Skopje,Division of Biology, Imperial College of Science Technology and Medicine, London, United Kingdom 3Institute of Polymers, Bulgarian Academy of Sciences, Bl. 103–A, Acad. G. Bonchev Str., 1113 Sofia,Hydrogels based on high molecular weight poly(ethylene oxide) (PEO) copolymers of ethylene oxide and propylene oxide, PEO/alginate and PEO/chitosan were synthesized by UV crosslinking of polymer aqueous solutions. These hydrogels were then characterized in terms of their gel fraction yield, degree of equilibrium swelling, shear storage and loss moduli. The physico-mechanical properties of the hydrogels were then correlated to their ability to sustain the viability and the activity of immobilized cells. The production of ethanol by immobilized Saccharomyces cerevisiae was used to test the suitability of the PEO based hydrogels as immobilization matrices. The PEO hydrogel with a value of 255 Pa for Gʹ and 11 Pa for Gʹʹ, showed the best mechanical properties of all the gels tested. Scanning electron microscope (SEM) analysis of the imobilized S. cerevisiae showed proliferation of the yeast cells entrapped inside the polymeric matrix.https://mjcce.org.mk/index.php/MJCCE/article/view/294hydrogelspoly(ethylene oxide)immobilizationentrapmentsaccharomyces cerevisiae |
| spellingShingle | Ruzica Jovanovic-Malinovska Samuel A. Amartey Slobodanka Kuzmanova Eleonora Winkelhausen Maja Cvetkovska Christo Tsvetanov The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cells Macedonian Journal of Chemistry and Chemical Engineering hydrogels poly(ethylene oxide) immobilization entrapment saccharomyces cerevisiae |
| title | The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cells |
| title_full | The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cells |
| title_fullStr | The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cells |
| title_full_unstemmed | The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cells |
| title_short | The use of poly(ethylene oxide) hydrogels as immobilization matrices for yeast cells |
| title_sort | use of poly ethylene oxide hydrogels as immobilization matrices for yeast cells |
| topic | hydrogels poly(ethylene oxide) immobilization entrapment saccharomyces cerevisiae |
| url | https://mjcce.org.mk/index.php/MJCCE/article/view/294 |
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