Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network.
Pericytes enveloping the endothelium play an important role in the physiology and pathology of microvessels, especially in vessel maturation and stabilization. However, our understanding of fundamental pericyte biology is limited by the lack of a robust in vitro model system that allows researchers...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4512698?pdf=render |
| _version_ | 1819262635412029440 |
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| author | Jaerim Kim Minhwan Chung Sudong Kim Dong Hyun Jo Jeong Hun Kim Noo Li Jeon |
| author_facet | Jaerim Kim Minhwan Chung Sudong Kim Dong Hyun Jo Jeong Hun Kim Noo Li Jeon |
| author_sort | Jaerim Kim |
| collection | DOAJ |
| description | Pericytes enveloping the endothelium play an important role in the physiology and pathology of microvessels, especially in vessel maturation and stabilization. However, our understanding of fundamental pericyte biology is limited by the lack of a robust in vitro model system that allows researchers to evaluate the interactions among multiple cell types in perfusable blood vessels. The present work describes a microfluidic platform that can be used to investigate interactions between pericytes and endothelial cells (ECs) during the sprouting, growth, and maturation steps of neovessel formation. A mixture of ECs and pericytes was attached to the side of a pre-patterned three dimensional fibrin matrix and allowed to sprout across the matrix. The effects of intact coverage and EC maturation by the pericytes on the perfused EC network were confirmed using a confocal microscope. Compared with EC monoculture conditions, EC-pericyte co-cultured vessels showed a significant reduction in diameter, increased numbers of junctions and branches and decreased permeability. In response to biochemical factors, ECs and pericytes in the platform showed the similar features with previous reports from in vivo experiments, thus reflect various pathophysiological conditions of in vivo microvessels. Taken together, these results support the physiological relevancy of our three-dimensional microfluidic culture system but also that the system can be used to screen drug effect on EC-pericyte biology. |
| first_indexed | 2024-12-23T20:00:50Z |
| format | Article |
| id | doaj.art-161f52b7eed44f5b9767089be16bbed4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T20:00:50Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-161f52b7eed44f5b9767089be16bbed42022-12-21T17:33:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013388010.1371/journal.pone.0133880Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network.Jaerim KimMinhwan ChungSudong KimDong Hyun JoJeong Hun KimNoo Li JeonPericytes enveloping the endothelium play an important role in the physiology and pathology of microvessels, especially in vessel maturation and stabilization. However, our understanding of fundamental pericyte biology is limited by the lack of a robust in vitro model system that allows researchers to evaluate the interactions among multiple cell types in perfusable blood vessels. The present work describes a microfluidic platform that can be used to investigate interactions between pericytes and endothelial cells (ECs) during the sprouting, growth, and maturation steps of neovessel formation. A mixture of ECs and pericytes was attached to the side of a pre-patterned three dimensional fibrin matrix and allowed to sprout across the matrix. The effects of intact coverage and EC maturation by the pericytes on the perfused EC network were confirmed using a confocal microscope. Compared with EC monoculture conditions, EC-pericyte co-cultured vessels showed a significant reduction in diameter, increased numbers of junctions and branches and decreased permeability. In response to biochemical factors, ECs and pericytes in the platform showed the similar features with previous reports from in vivo experiments, thus reflect various pathophysiological conditions of in vivo microvessels. Taken together, these results support the physiological relevancy of our three-dimensional microfluidic culture system but also that the system can be used to screen drug effect on EC-pericyte biology.http://europepmc.org/articles/PMC4512698?pdf=render |
| spellingShingle | Jaerim Kim Minhwan Chung Sudong Kim Dong Hyun Jo Jeong Hun Kim Noo Li Jeon Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. PLoS ONE |
| title | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. |
| title_full | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. |
| title_fullStr | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. |
| title_full_unstemmed | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. |
| title_short | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. |
| title_sort | engineering of a biomimetic pericyte covered 3d microvascular network |
| url | http://europepmc.org/articles/PMC4512698?pdf=render |
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