Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo
Summary: Neocortical layer 6 (L6) is less understood than other more superficial layers, largely owing to limitations of performing high-resolution investigations in vivo. Here, we show that labeling with the Challenge Virus Standard (CVS) rabies virus strain enables high-quality imaging of L6 neuro...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223007022 |
| _version_ | 1797842419095437312 |
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| author | Miaoqing Gu Xiuli Li Shanshan Liang Jiahui Zhu Pei Sun Yong He Haipeng Yu Ruijie Li Zhenqiao Zhou Jing Lyu Sunny C. Li Eike Budinger Yi Zhou Hongbo Jia Jianxiong Zhang Xiaowei Chen |
| author_facet | Miaoqing Gu Xiuli Li Shanshan Liang Jiahui Zhu Pei Sun Yong He Haipeng Yu Ruijie Li Zhenqiao Zhou Jing Lyu Sunny C. Li Eike Budinger Yi Zhou Hongbo Jia Jianxiong Zhang Xiaowei Chen |
| author_sort | Miaoqing Gu |
| collection | DOAJ |
| description | Summary: Neocortical layer 6 (L6) is less understood than other more superficial layers, largely owing to limitations of performing high-resolution investigations in vivo. Here, we show that labeling with the Challenge Virus Standard (CVS) rabies virus strain enables high-quality imaging of L6 neurons by conventional two-photon microscopes. CVS virus injection into the medial geniculate body can selectively label L6 neurons in the auditory cortex. Only three days after injection, dendrites and cell bodies of L6 neurons could be imaged across all cortical layers. Ca2+ imaging in awake mice showed that sound stimulation evokes neuronal responses from cell bodies with minimal contamination from neuropil signals. In addition, dendritic Ca2+ imaging revealed significant responses from spines and trunks across all layers. These results demonstrate a reliable method capable of rapid, high-quality labeling of L6 neurons that can be readily extended to other brain regions. |
| first_indexed | 2024-04-09T16:47:36Z |
| format | Article |
| id | doaj.art-162102caa9994681af40a29f55071d14 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T16:47:36Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-162102caa9994681af40a29f55071d142023-04-22T06:22:20ZengElsevieriScience2589-00422023-05-01265106625Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivoMiaoqing Gu0Xiuli Li1Shanshan Liang2Jiahui Zhu3Pei Sun4Yong He5Haipeng Yu6Ruijie Li7Zhenqiao Zhou8Jing Lyu9Sunny C. Li10Eike Budinger11Yi Zhou12Hongbo Jia13Jianxiong Zhang14Xiaowei Chen15School of Physical Science and Technology, Guangxi University, Nanning 530004, China; Advanced Institute for Brain and Intelligence, School of Medicine, Guangxi University, Nanning 530004, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, ChinaAdvanced Institute for Brain and Intelligence, School of Medicine, Guangxi University, Nanning 530004, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, ChinaDepartment of Neurobiology, Chongqing Key Laboratory of Neurobiology, School of Basic Medicine, Third Military Medical University, Chongqing 400038, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaAdvanced Institute for Brain and Intelligence, School of Medicine, Guangxi University, Nanning 530004, ChinaCombinatorial NeuroImaging Core Facility, Leibniz Institute for Neurobiology, 39118 Magdeburg, GermanyAdvanced Institute for Brain and Intelligence, School of Medicine, Guangxi University, Nanning 530004, China; Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, School of Basic Medicine, Third Military Medical University, Chongqing 400038, China; Corresponding authorSchool of Physical Science and Technology, Guangxi University, Nanning 530004, China; Brain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China; Combinatorial NeuroImaging Core Facility, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany; Corresponding authorBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, China; Corresponding authorBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, China; Guangyang Bay Laboratory, Chongqing Institute for Brain and Intelligence, Chongqing 400064, China; Corresponding authorSummary: Neocortical layer 6 (L6) is less understood than other more superficial layers, largely owing to limitations of performing high-resolution investigations in vivo. Here, we show that labeling with the Challenge Virus Standard (CVS) rabies virus strain enables high-quality imaging of L6 neurons by conventional two-photon microscopes. CVS virus injection into the medial geniculate body can selectively label L6 neurons in the auditory cortex. Only three days after injection, dendrites and cell bodies of L6 neurons could be imaged across all cortical layers. Ca2+ imaging in awake mice showed that sound stimulation evokes neuronal responses from cell bodies with minimal contamination from neuropil signals. In addition, dendritic Ca2+ imaging revealed significant responses from spines and trunks across all layers. These results demonstrate a reliable method capable of rapid, high-quality labeling of L6 neurons that can be readily extended to other brain regions.http://www.sciencedirect.com/science/article/pii/S2589004223007022Optical imagingMolecular neuroscienceCellular neuroscience |
| spellingShingle | Miaoqing Gu Xiuli Li Shanshan Liang Jiahui Zhu Pei Sun Yong He Haipeng Yu Ruijie Li Zhenqiao Zhou Jing Lyu Sunny C. Li Eike Budinger Yi Zhou Hongbo Jia Jianxiong Zhang Xiaowei Chen Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo iScience Optical imaging Molecular neuroscience Cellular neuroscience |
| title | Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo |
| title_full | Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo |
| title_fullStr | Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo |
| title_full_unstemmed | Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo |
| title_short | Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo |
| title_sort | rabies virus based labeling of layer 6 corticothalamic neurons for two photon imaging in vivo |
| topic | Optical imaging Molecular neuroscience Cellular neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223007022 |
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