Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes
Organic amendment is important for promoting soil quality through increasing soil fertility and soil microbes. This study evaluated the effectiveness of using liquid food waste material (LFM) as a microbial resource, by analyzing the microbial community composition in LFM, and by isolating plant gro...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/7/954 |
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| author | Waleed Asghar Shiho Kondo Riho Iguchi Ahmad Mahmood Ryota Kataoka |
| author_facet | Waleed Asghar Shiho Kondo Riho Iguchi Ahmad Mahmood Ryota Kataoka |
| author_sort | Waleed Asghar |
| collection | DOAJ |
| description | Organic amendment is important for promoting soil quality through increasing soil fertility and soil microbes. This study evaluated the effectiveness of using liquid food waste material (LFM) as a microbial resource, by analyzing the microbial community composition in LFM, and by isolating plant growth-promoting bacteria (PGPB) from the material. High-throughput sequencing of LFM, collected every month from May to September 2018, resulted in the detection of >1000 bacterial operational taxonomic units (OTUs) in the LFM. The results showed that <i>Firmicutes</i> was abundant and most frequently detected, followed by <i>Proteobacteria</i> and <i>Actinobacteria.</i> Of the culturable strains isolated from LFM, almost all belonged to the genus <i>Bacillus</i>. Four strains of PGPB were selected from the isolated strains, with traits such as indole acetic acid production and 1-aminocyclopropane-1-carboxylic acid deaminase activity. Lettuce growth was improved via LFM amendment with PGPB, and <i>Brassica</i> <i>rapa</i> showed significant differences in root biomass when LFM amendment was compared with the use chemical fertilizer. Field experiments using LFM showed slight differences in growth for <i>Brassica rapa,</i> lettuce and eggplant, when compared with the use of chemical fertilizer. LFM is a useful microbial resource for the isolation of PGPB, and its use as fertilizer could result in reduced chemical fertilizer usage in sustainable agriculture. |
| first_indexed | 2024-03-10T18:43:15Z |
| format | Article |
| id | doaj.art-1622fd61b7d446b994be237452b04d65 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T18:43:15Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-1622fd61b7d446b994be237452b04d652023-11-20T05:39:51ZengMDPI AGAgronomy2073-43952020-07-0110795410.3390/agronomy10070954Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting MicrobesWaleed Asghar0Shiho Kondo1Riho Iguchi2Ahmad Mahmood3Ryota Kataoka4Department of Environmental Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, JapanDepartment of Environmental Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, JapanDepartment of Environmental Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, JapanDepartment of Environmental Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, JapanDepartment of Environmental Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, JapanOrganic amendment is important for promoting soil quality through increasing soil fertility and soil microbes. This study evaluated the effectiveness of using liquid food waste material (LFM) as a microbial resource, by analyzing the microbial community composition in LFM, and by isolating plant growth-promoting bacteria (PGPB) from the material. High-throughput sequencing of LFM, collected every month from May to September 2018, resulted in the detection of >1000 bacterial operational taxonomic units (OTUs) in the LFM. The results showed that <i>Firmicutes</i> was abundant and most frequently detected, followed by <i>Proteobacteria</i> and <i>Actinobacteria.</i> Of the culturable strains isolated from LFM, almost all belonged to the genus <i>Bacillus</i>. Four strains of PGPB were selected from the isolated strains, with traits such as indole acetic acid production and 1-aminocyclopropane-1-carboxylic acid deaminase activity. Lettuce growth was improved via LFM amendment with PGPB, and <i>Brassica</i> <i>rapa</i> showed significant differences in root biomass when LFM amendment was compared with the use chemical fertilizer. Field experiments using LFM showed slight differences in growth for <i>Brassica rapa,</i> lettuce and eggplant, when compared with the use of chemical fertilizer. LFM is a useful microbial resource for the isolation of PGPB, and its use as fertilizer could result in reduced chemical fertilizer usage in sustainable agriculture.https://www.mdpi.com/2073-4395/10/7/954bacterial community compositionliquid food waste materials (LFM)plant growth-promoting bacteria (PGPB)plant growth-promoting (PGP) traits |
| spellingShingle | Waleed Asghar Shiho Kondo Riho Iguchi Ahmad Mahmood Ryota Kataoka Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes Agronomy bacterial community composition liquid food waste materials (LFM) plant growth-promoting bacteria (PGPB) plant growth-promoting (PGP) traits |
| title | Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes |
| title_full | Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes |
| title_fullStr | Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes |
| title_full_unstemmed | Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes |
| title_short | Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes |
| title_sort | agricultural utilization of unused resources liquid food waste material as a new source of plant growth promoting microbes |
| topic | bacterial community composition liquid food waste materials (LFM) plant growth-promoting bacteria (PGPB) plant growth-promoting (PGP) traits |
| url | https://www.mdpi.com/2073-4395/10/7/954 |
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