DIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUM
Introduction and aim: In recent decades, many factors such as smoking, unhealthy diet as well as high alcohol intake were marked as risk factors that can lead to increased incidence of malignant alterations, gingivitis, periodontal disease and other oral epithelium pathological changes. Having in mi...
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Format: | Article |
Language: | English |
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Association of medical doctors Sanamed Novi Pazar
2016-12-01
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Series: | Sanamed |
Online Access: | http://www.sanamed.rs/OJS/index.php/Sanamed/article/view/137/69 |
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author | Tanaskovic Stankovic Sanja Cabunac Jovan Kanjevac Tatjana Milosavljević Zoran |
author_facet | Tanaskovic Stankovic Sanja Cabunac Jovan Kanjevac Tatjana Milosavljević Zoran |
author_sort | Tanaskovic Stankovic Sanja |
collection | DOAJ |
description | Introduction and aim: In recent decades, many factors such as smoking, unhealthy diet as well as high alcohol intake were marked as risk factors that can lead to increased incidence of malignant alterations, gingivitis, periodontal disease and other oral epithelium pathological changes. Having in mind that in the group of non-malignant and non-dental oral pathology gingivitis and periodontal disease are the most common oral mucosa alterations aim of our research was to investigate histomorphometric characteristics of healthy and altered oral and gingival epithelium.
Material and methods: Tissue samples of 24 oral and gingival mucosa specimens were collected. Samples were fixed in 10% buffered paraformaldehyde, routinely processed and embedded in paraffin blocks. From each block sections 5 micrometer thin were made and standard H/E staining as well as immunocytochemical detection of Ki-67 proliferation marker and CD79a lymphocyte marker were performed. Measurements and image analysis was performed with Image Pro Plus software (Media Cybernetics, USA) and Axiovision (Ziess, USA).
Results: We showed that inflamed gingival epithelium is increasing its thickness in proportion to the severity of adjacent inflammation. Furthermore, mitotic index is rising (up to 132%) in the same manner as well as basal lamina length (up to 70%) when normal and inflamed gingiva is compared. Architecture of epithelial ridges is changed from straightforward to mesh-like.
Conclusion: Assessment of the free gingival epithelium thickness is directly related to the severity of the inflammation process i |
first_indexed | 2024-03-07T17:53:30Z |
format | Article |
id | doaj.art-16234c1d913b4a3f8fc831fa6adc1f0a |
institution | Directory Open Access Journal |
issn | 1452-662X 2217-8171 |
language | English |
last_indexed | 2024-03-07T17:53:30Z |
publishDate | 2016-12-01 |
publisher | Association of medical doctors Sanamed Novi Pazar |
record_format | Article |
series | Sanamed |
spelling | doaj.art-16234c1d913b4a3f8fc831fa6adc1f0a2024-03-02T13:08:15ZengAssociation of medical doctors Sanamed Novi PazarSanamed1452-662X2217-81712016-12-0111319720110.5937/sanamed1603197TDIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUMTanaskovic Stankovic Sanja 0 Cabunac Jovan1Kanjevac Tatjana2Milosavljević Zoran3Histology and embryology Institute, Faculty of Medical Sciences, University of Kragujevac, SerbiaDepartment for Preventive and Pediatric Dentistry, Faculty of Medicine, University of Kragujevac, SerbiaDepartment for Preventive and Pediatric Dentistry, Faculty of Medicine, University of Kragujevac, SerbiaHistology and embryology Institute, Faculty of Medical Sciences, University of Kragujevac, SerbiaIntroduction and aim: In recent decades, many factors such as smoking, unhealthy diet as well as high alcohol intake were marked as risk factors that can lead to increased incidence of malignant alterations, gingivitis, periodontal disease and other oral epithelium pathological changes. Having in mind that in the group of non-malignant and non-dental oral pathology gingivitis and periodontal disease are the most common oral mucosa alterations aim of our research was to investigate histomorphometric characteristics of healthy and altered oral and gingival epithelium. Material and methods: Tissue samples of 24 oral and gingival mucosa specimens were collected. Samples were fixed in 10% buffered paraformaldehyde, routinely processed and embedded in paraffin blocks. From each block sections 5 micrometer thin were made and standard H/E staining as well as immunocytochemical detection of Ki-67 proliferation marker and CD79a lymphocyte marker were performed. Measurements and image analysis was performed with Image Pro Plus software (Media Cybernetics, USA) and Axiovision (Ziess, USA). Results: We showed that inflamed gingival epithelium is increasing its thickness in proportion to the severity of adjacent inflammation. Furthermore, mitotic index is rising (up to 132%) in the same manner as well as basal lamina length (up to 70%) when normal and inflamed gingiva is compared. Architecture of epithelial ridges is changed from straightforward to mesh-like. Conclusion: Assessment of the free gingival epithelium thickness is directly related to the severity of the inflammation process ihttp://www.sanamed.rs/OJS/index.php/Sanamed/article/view/137/69 |
spellingShingle | Tanaskovic Stankovic Sanja Cabunac Jovan Kanjevac Tatjana Milosavljević Zoran DIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUM Sanamed |
title | DIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUM |
title_full | DIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUM |
title_fullStr | DIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUM |
title_full_unstemmed | DIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUM |
title_short | DIFFERENTIAL HISTOMORPHOMETRIC CHANGES IN NORMAL AND INFLAMED GINGIVAL EPITHELIUM |
title_sort | differential histomorphometric changes in normal and inflamed gingival epithelium |
url | http://www.sanamed.rs/OJS/index.php/Sanamed/article/view/137/69 |
work_keys_str_mv | AT tanaskovicstankovicsanja differentialhistomorphometricchangesinnormalandinflamedgingivalepithelium AT cabunacjovan differentialhistomorphometricchangesinnormalandinflamedgingivalepithelium AT kanjevactatjana differentialhistomorphometricchangesinnormalandinflamedgingivalepithelium AT milosavljeviczoran differentialhistomorphometricchangesinnormalandinflamedgingivalepithelium |