The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup
Abstract Background The existence of mitochondria-related organelles (MROs) is proposed for eukaryotic organisms. The Amoebozoa includes some organisms that are known to have mitosomes but also organisms that have aerobic mitochondria. However, the mitochondrial protein apparatus of this supergroup...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-017-4383-1 |
| _version_ | 1819076660205453312 |
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| author | Małgorzata Wojtkowska Dorota Buczek Yutaka Suzuki Victoria Shabardina Wojciech Makałowski Hanna Kmita |
| author_facet | Małgorzata Wojtkowska Dorota Buczek Yutaka Suzuki Victoria Shabardina Wojciech Makałowski Hanna Kmita |
| author_sort | Małgorzata Wojtkowska |
| collection | DOAJ |
| description | Abstract Background The existence of mitochondria-related organelles (MROs) is proposed for eukaryotic organisms. The Amoebozoa includes some organisms that are known to have mitosomes but also organisms that have aerobic mitochondria. However, the mitochondrial protein apparatus of this supergroup remains largely unsampled, except for the mitochondrial outer membrane import complexes studied recently. Therefore, in this study we investigated the mitochondrial inner membrane and intermembrane space complexes, using the available genome and transcriptome sequences. Results When compared with the canonical cognate complexes described for the yeast Saccharomyces cerevisiae, amoebozoans with aerobic mitochondria, display lower differences in the number of subunits predicted for these complexes than the mitochondrial outer membrane complexes, although the predicted subunits appear to display different levels of diversity in regard to phylogenetic position and isoform numbers. For the putative mitosome-bearing amoebozoans, the number of predicted subunits suggests the complex elimination distinctly more pronounced than in the case of the outer membrane ones. Conclusion The results concern the problem of mitochondrial and mitosome protein import machinery structural variability and the reduction of their complexity within the currently defined supergroup of Amoebozoa. This results are crucial for better understanding of the Amoebozoa taxa of both biomedical and evolutionary importance. |
| first_indexed | 2024-12-21T18:44:50Z |
| format | Article |
| id | doaj.art-162354b2385c4e47b4a025212e096875 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-21T18:44:50Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-162354b2385c4e47b4a025212e0968752022-12-21T18:53:55ZengBMCBMC Genomics1471-21642017-12-0118111410.1186/s12864-017-4383-1The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroupMałgorzata Wojtkowska0Dorota Buczek1Yutaka Suzuki2Victoria Shabardina3Wojciech Makałowski4Hanna Kmita5Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz UniversityLaboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz UniversityDepartment of Medical Genome Sciences, Graduate School of Frontier Sciences, University of TokyoInstitute of Bioinformatics, Faculty of Medicine, University of MuensterInstitute of Bioinformatics, Faculty of Medicine, University of MuensterLaboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz UniversityAbstract Background The existence of mitochondria-related organelles (MROs) is proposed for eukaryotic organisms. The Amoebozoa includes some organisms that are known to have mitosomes but also organisms that have aerobic mitochondria. However, the mitochondrial protein apparatus of this supergroup remains largely unsampled, except for the mitochondrial outer membrane import complexes studied recently. Therefore, in this study we investigated the mitochondrial inner membrane and intermembrane space complexes, using the available genome and transcriptome sequences. Results When compared with the canonical cognate complexes described for the yeast Saccharomyces cerevisiae, amoebozoans with aerobic mitochondria, display lower differences in the number of subunits predicted for these complexes than the mitochondrial outer membrane complexes, although the predicted subunits appear to display different levels of diversity in regard to phylogenetic position and isoform numbers. For the putative mitosome-bearing amoebozoans, the number of predicted subunits suggests the complex elimination distinctly more pronounced than in the case of the outer membrane ones. Conclusion The results concern the problem of mitochondrial and mitosome protein import machinery structural variability and the reduction of their complexity within the currently defined supergroup of Amoebozoa. This results are crucial for better understanding of the Amoebozoa taxa of both biomedical and evolutionary importance.http://link.springer.com/article/10.1186/s12864-017-4383-1AmoebozoaMitochondriaMitosomesProtein importTIM22 complexTIM23 complex |
| spellingShingle | Małgorzata Wojtkowska Dorota Buczek Yutaka Suzuki Victoria Shabardina Wojciech Makałowski Hanna Kmita The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup BMC Genomics Amoebozoa Mitochondria Mitosomes Protein import TIM22 complex TIM23 complex |
| title | The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup |
| title_full | The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup |
| title_fullStr | The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup |
| title_full_unstemmed | The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup |
| title_short | The emerging picture of the mitochondrial protein import complexes of Amoebozoa supergroup |
| title_sort | emerging picture of the mitochondrial protein import complexes of amoebozoa supergroup |
| topic | Amoebozoa Mitochondria Mitosomes Protein import TIM22 complex TIM23 complex |
| url | http://link.springer.com/article/10.1186/s12864-017-4383-1 |
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