Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker Rats
Ruminant fats are characterized by different levels of conjugated linoleic acid (CLA) and α-linolenic acid (18:3n-3, ALA), according to animal diet. Tissue fatty acids and their N-acylethanolamides were analyzed in male obese Zucker rats fed diets containing lamb meat fat with different fat...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-11-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/9/11/751 |
| _version_ | 1828924867223748608 |
|---|---|
| author | Gianfranca Carta Elisabetta Murru Claudia Manca Andrea Serra Marcello Mele Sebastiano Banni |
| author_facet | Gianfranca Carta Elisabetta Murru Claudia Manca Andrea Serra Marcello Mele Sebastiano Banni |
| author_sort | Gianfranca Carta |
| collection | DOAJ |
| description | Ruminant fats are characterized by different levels of conjugated linoleic acid (CLA) and α-linolenic acid (18:3n-3, ALA), according to animal diet. Tissue fatty acids and their N-acylethanolamides were analyzed in male obese Zucker rats fed diets containing lamb meat fat with different fatty acid profiles: (A) enriched in CLA; (B) enriched in ALA and low in CLA; (C) low in ALA and CLA; and one containing a mixture of olive and corn oils: (D) high in linoleic acid (18:2n-6, LA) and ALA, in order to evaluate early lipid metabolism markers. No changes in body and liver weights were observed. CLA and ALA were incorporated into most tissues, mirroring the dietary content; eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increased according to dietary ALA, which was strongly influenced by CLA. The n-3 highly-unsaturated fatty acid (HUFA) score, biomarker of the n-3/n-6 fatty acid ratio, was increased in tissues of rats fed animal fats high in CLA and/or ALA compared to those fed vegetable fat. DHA and CLA were associated with a significant increase in oleoylethanolamide and decrease in anandamide in subcutaneous fat. The results showed that meat fat nutritional values are strongly influenced by their CLA and ALA contents, modulating the tissue n-3 HUFA score. |
| first_indexed | 2024-12-13T23:04:51Z |
| format | Article |
| id | doaj.art-1626274e55c645d2b063409d64dd5f7c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-13T23:04:51Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1626274e55c645d2b063409d64dd5f7c2022-12-21T23:28:19ZengMDPI AGBiomolecules2218-273X2019-11-0191175110.3390/biom9110751biom9110751Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker RatsGianfranca Carta0Elisabetta Murru1Claudia Manca2Andrea Serra3Marcello Mele4Sebastiano Banni5Department of Biomedical Sciences, University of Cagliari, 09042 Monserrato, CA, ItalyDepartment of Biomedical Sciences, University of Cagliari, 09042 Monserrato, CA, ItalyDepartment of Biomedical Sciences, University of Cagliari, 09042 Monserrato, CA, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, ItalyDepartment of Biomedical Sciences, University of Cagliari, 09042 Monserrato, CA, ItalyRuminant fats are characterized by different levels of conjugated linoleic acid (CLA) and α-linolenic acid (18:3n-3, ALA), according to animal diet. Tissue fatty acids and their N-acylethanolamides were analyzed in male obese Zucker rats fed diets containing lamb meat fat with different fatty acid profiles: (A) enriched in CLA; (B) enriched in ALA and low in CLA; (C) low in ALA and CLA; and one containing a mixture of olive and corn oils: (D) high in linoleic acid (18:2n-6, LA) and ALA, in order to evaluate early lipid metabolism markers. No changes in body and liver weights were observed. CLA and ALA were incorporated into most tissues, mirroring the dietary content; eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increased according to dietary ALA, which was strongly influenced by CLA. The n-3 highly-unsaturated fatty acid (HUFA) score, biomarker of the n-3/n-6 fatty acid ratio, was increased in tissues of rats fed animal fats high in CLA and/or ALA compared to those fed vegetable fat. DHA and CLA were associated with a significant increase in oleoylethanolamide and decrease in anandamide in subcutaneous fat. The results showed that meat fat nutritional values are strongly influenced by their CLA and ALA contents, modulating the tissue n-3 HUFA score.https://www.mdpi.com/2218-273X/9/11/751claconjugated linoleic acidalaα-linolenic acidn-3 hufa scoremeat fatvegetable fat |
| spellingShingle | Gianfranca Carta Elisabetta Murru Claudia Manca Andrea Serra Marcello Mele Sebastiano Banni Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker Rats Biomolecules cla conjugated linoleic acid ala α-linolenic acid n-3 hufa score meat fat vegetable fat |
| title | Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker Rats |
| title_full | Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker Rats |
| title_fullStr | Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker Rats |
| title_full_unstemmed | Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker Rats |
| title_short | Natural CLA-Enriched Lamb Meat Fat Modifies Tissue Fatty Acid Profile and Increases n-3 HUFA Score in Obese Zucker Rats |
| title_sort | natural cla enriched lamb meat fat modifies tissue fatty acid profile and increases n 3 hufa score in obese zucker rats |
| topic | cla conjugated linoleic acid ala α-linolenic acid n-3 hufa score meat fat vegetable fat |
| url | https://www.mdpi.com/2218-273X/9/11/751 |
| work_keys_str_mv | AT gianfrancacarta naturalclaenrichedlambmeatfatmodifiestissuefattyacidprofileandincreasesn3hufascoreinobesezuckerrats AT elisabettamurru naturalclaenrichedlambmeatfatmodifiestissuefattyacidprofileandincreasesn3hufascoreinobesezuckerrats AT claudiamanca naturalclaenrichedlambmeatfatmodifiestissuefattyacidprofileandincreasesn3hufascoreinobesezuckerrats AT andreaserra naturalclaenrichedlambmeatfatmodifiestissuefattyacidprofileandincreasesn3hufascoreinobesezuckerrats AT marcellomele naturalclaenrichedlambmeatfatmodifiestissuefattyacidprofileandincreasesn3hufascoreinobesezuckerrats AT sebastianobanni naturalclaenrichedlambmeatfatmodifiestissuefattyacidprofileandincreasesn3hufascoreinobesezuckerrats |