Tumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breast
Abstract Background In this study, we investigated CD20+ TILs in triple-negative breast cancer (TNBC) and their relationship with T lymphocyte subsets (CD4+, CD8+, CD25+, and FOXP3+), including their combined prognostic value using an immunohistochemical staining method. Methods We investigated 107...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-03-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-021-08009-x |
| _version_ | 1818728748267077632 |
|---|---|
| author | Hajime Kuroda Tsengelmaa Jamiyan Rin Yamaguchi Akinari Kakumoto Akihito Abe Oi Harada Atsuko Masunaga |
| author_facet | Hajime Kuroda Tsengelmaa Jamiyan Rin Yamaguchi Akinari Kakumoto Akihito Abe Oi Harada Atsuko Masunaga |
| author_sort | Hajime Kuroda |
| collection | DOAJ |
| description | Abstract Background In this study, we investigated CD20+ TILs in triple-negative breast cancer (TNBC) and their relationship with T lymphocyte subsets (CD4+, CD8+, CD25+, and FOXP3+), including their combined prognostic value using an immunohistochemical staining method. Methods We investigated 107 patients with TNBC for whom a full-face section stained by hematoxylin and eosin between 2006 and 2018 at Dokkyo Medical University Hospital was available. Results The strongest association of infiltrating CD20+ TILs was with CD4+ TILs. There was a significant relationship between CD20+ and CD4+ TILs (r = 0.177; p < 0.001), CD8+ TILs (r = 0.085; p = 0.002), and FOXP3+ TILs (r = 0.0043; p = 0.032). No significant relationships were observed between the CD20+ and CD25+ TILs (r = 0.012; p = 0.264). Multivariate analysis revealed that only the CD20+/FOXP3 ratio was an independent factor for relapse-free survival (p < 0.001) and overall survival (p < 0.001). Patients with tumors highly infiltrated by CD4+, CD8+, and CD20+ TILs had a good prognosis. In contrast, those with tumors weakly infiltrated by CD20+ TILs but highly infiltrated by CD25+ and FOXP3+ TILs had a poor prognosis. Conclusions CD20+ TILs may support an increase in CD4+ and CD8+ TILs, which altered the anti-tumor response, resulting in a positive prognosis. CD20+ TILs correlated with FOXP3+ Treg lymphocytes, which were reported to be correlated with a poor prognosis. Our study suggested that TIL-B cells have dual and conflicting roles in TIL-T immune reactions in TNBC. |
| first_indexed | 2024-12-17T22:34:55Z |
| format | Article |
| id | doaj.art-162ca5d5a81a4077be0deae2afc3dc99 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-17T22:34:55Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-162ca5d5a81a4077be0deae2afc3dc992022-12-21T21:30:06ZengBMCBMC Cancer1471-24072021-03-0121111010.1186/s12885-021-08009-xTumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breastHajime Kuroda0Tsengelmaa Jamiyan1Rin Yamaguchi2Akinari Kakumoto3Akihito Abe4Oi Harada5Atsuko Masunaga6Department of Diagnostic Pathology, Tokyo Women’s Medical University, Medical Center EastDepartment of Diagnostic Pathology, Dokkyo Medical UniversityDepartment of Pathology & Laboratory Medicine, Kurume University Medical CenterDepartment of Diagnostic Pathology, Tokyo Women’s Medical University, Medical Center EastBreast Center, Dokkyo Medical UniversityBreast center, Showa UniversityDepartment of Diagnostic Pathology, Tokyo Women’s Medical University, Medical Center EastAbstract Background In this study, we investigated CD20+ TILs in triple-negative breast cancer (TNBC) and their relationship with T lymphocyte subsets (CD4+, CD8+, CD25+, and FOXP3+), including their combined prognostic value using an immunohistochemical staining method. Methods We investigated 107 patients with TNBC for whom a full-face section stained by hematoxylin and eosin between 2006 and 2018 at Dokkyo Medical University Hospital was available. Results The strongest association of infiltrating CD20+ TILs was with CD4+ TILs. There was a significant relationship between CD20+ and CD4+ TILs (r = 0.177; p < 0.001), CD8+ TILs (r = 0.085; p = 0.002), and FOXP3+ TILs (r = 0.0043; p = 0.032). No significant relationships were observed between the CD20+ and CD25+ TILs (r = 0.012; p = 0.264). Multivariate analysis revealed that only the CD20+/FOXP3 ratio was an independent factor for relapse-free survival (p < 0.001) and overall survival (p < 0.001). Patients with tumors highly infiltrated by CD4+, CD8+, and CD20+ TILs had a good prognosis. In contrast, those with tumors weakly infiltrated by CD20+ TILs but highly infiltrated by CD25+ and FOXP3+ TILs had a poor prognosis. Conclusions CD20+ TILs may support an increase in CD4+ and CD8+ TILs, which altered the anti-tumor response, resulting in a positive prognosis. CD20+ TILs correlated with FOXP3+ Treg lymphocytes, which were reported to be correlated with a poor prognosis. Our study suggested that TIL-B cells have dual and conflicting roles in TIL-T immune reactions in TNBC.https://doi.org/10.1186/s12885-021-08009-xBreastTriple-negative cancerTumor-infiltrating B lymphocytesTumor-infiltrating T lymphocytes |
| spellingShingle | Hajime Kuroda Tsengelmaa Jamiyan Rin Yamaguchi Akinari Kakumoto Akihito Abe Oi Harada Atsuko Masunaga Tumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breast BMC Cancer Breast Triple-negative cancer Tumor-infiltrating B lymphocytes Tumor-infiltrating T lymphocytes |
| title | Tumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breast |
| title_full | Tumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breast |
| title_fullStr | Tumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breast |
| title_full_unstemmed | Tumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breast |
| title_short | Tumor-infiltrating B cells and T cells correlate with postoperative prognosis in triple-negative carcinoma of the breast |
| title_sort | tumor infiltrating b cells and t cells correlate with postoperative prognosis in triple negative carcinoma of the breast |
| topic | Breast Triple-negative cancer Tumor-infiltrating B lymphocytes Tumor-infiltrating T lymphocytes |
| url | https://doi.org/10.1186/s12885-021-08009-x |
| work_keys_str_mv | AT hajimekuroda tumorinfiltratingbcellsandtcellscorrelatewithpostoperativeprognosisintriplenegativecarcinomaofthebreast AT tsengelmaajamiyan tumorinfiltratingbcellsandtcellscorrelatewithpostoperativeprognosisintriplenegativecarcinomaofthebreast AT rinyamaguchi tumorinfiltratingbcellsandtcellscorrelatewithpostoperativeprognosisintriplenegativecarcinomaofthebreast AT akinarikakumoto tumorinfiltratingbcellsandtcellscorrelatewithpostoperativeprognosisintriplenegativecarcinomaofthebreast AT akihitoabe tumorinfiltratingbcellsandtcellscorrelatewithpostoperativeprognosisintriplenegativecarcinomaofthebreast AT oiharada tumorinfiltratingbcellsandtcellscorrelatewithpostoperativeprognosisintriplenegativecarcinomaofthebreast AT atsukomasunaga tumorinfiltratingbcellsandtcellscorrelatewithpostoperativeprognosisintriplenegativecarcinomaofthebreast |