Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells
Statement of the Problem: The stimulation of odontogenic activity is considered an essential property for biomaterials used in vital pulp therapy. Purpose: The present study aimed to evaluate the effect of the incorporation of zeolite containing silver-zinc nanoparticles (Ze-Ag-Zn) into Angelous mi...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2021-09-01
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| Series: | Journal of Dentistry |
| Subjects: | |
| Online Access: | https://dentjods.sums.ac.ir/article_47200_7c28f43e4f4da60c4edd8fd05c4e845f.pdf |
| _version_ | 1819131473339351040 |
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| author | Negin Ghasemi Sadegh Salarinasab Reza Rahbarghazi Samin Sedghi Paria Davoudi |
| author_facet | Negin Ghasemi Sadegh Salarinasab Reza Rahbarghazi Samin Sedghi Paria Davoudi |
| author_sort | Negin Ghasemi |
| collection | DOAJ |
| description | Statement of the Problem: The stimulation of odontogenic activity is considered an essential property for biomaterials used in vital pulp therapy.
Purpose: The present study aimed to evaluate the effect of the incorporation of zeolite containing silver-zinc nanoparticles (Ze-Ag-Zn) into Angelous mineral trioxide aggregate (AMTA) on the odontogenic activity of human dental pulp stem cells (HDPSCs).
Materials and Method: In this in vitro study, HDPSCs were treated with 2% wt of synthesized Ze-Ag-Zn particles+AMTA, AMTA and Ze-Ag-Zn disks. The negative control cells did not receive any treatment. Then, cell viability was measured using the MTT assay after 7 and 14 days of the treatment course. The alkaline phosphatase (ALP) activity and calcium ion level were also measured in the supernatant culture media using auto-analyzer kits. The obtained data were analyzed using one-way ANOVA and Student t-test where appropriate. The level of the statistical significance was set at p< 0.05.
Results: The results indicated that HDPSCs treated with AMTA and AMTA+Ze-Ag-Zn particles did not show any significant cell death compared with the control cells after 14 days of the treatment course while the ALP activity and calcium ion levels were significantly (p< 0.05) elevated. Also, the addition of AMTA particles to the cell culture media resulted in increased ALP activity and calcium ion level compared with HDPSCs treated with AMTA+ Ze-Ag-Zn particles on day 7 of the treatment course (p< 0.05).
Conclusion: It seems that the incorporation of Ze-Ag-Zn particles into AMTA did not have any significant positive effect on the biomineralization properties of AMTA. |
| first_indexed | 2024-12-22T09:16:04Z |
| format | Article |
| id | doaj.art-162cc1d4e06e407081e8ad143d617a45 |
| institution | Directory Open Access Journal |
| issn | 2345-6485 2345-6418 |
| language | English |
| last_indexed | 2024-12-22T09:16:04Z |
| publishDate | 2021-09-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Dentistry |
| spelling | doaj.art-162cc1d4e06e407081e8ad143d617a452022-12-21T18:31:18ZengShiraz University of Medical SciencesJournal of Dentistry2345-64852345-64182021-09-01223187192Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem CellsNegin Ghasemi 0Sadegh Salarinasab 1Reza Rahbarghazi 2Samin Sedghi 3Paria Davoudi 4Dept. of Endodontics, Dental and Periodontal Research Center, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran. Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Science, Kerman, Iran.Dept. of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Private Office, Tabriz, Iran. Dept. of Endodontics, Dental Faculty, Shahid Beheshti Medical University, Tehran, IranStatement of the Problem: The stimulation of odontogenic activity is considered an essential property for biomaterials used in vital pulp therapy. Purpose: The present study aimed to evaluate the effect of the incorporation of zeolite containing silver-zinc nanoparticles (Ze-Ag-Zn) into Angelous mineral trioxide aggregate (AMTA) on the odontogenic activity of human dental pulp stem cells (HDPSCs). Materials and Method: In this in vitro study, HDPSCs were treated with 2% wt of synthesized Ze-Ag-Zn particles+AMTA, AMTA and Ze-Ag-Zn disks. The negative control cells did not receive any treatment. Then, cell viability was measured using the MTT assay after 7 and 14 days of the treatment course. The alkaline phosphatase (ALP) activity and calcium ion level were also measured in the supernatant culture media using auto-analyzer kits. The obtained data were analyzed using one-way ANOVA and Student t-test where appropriate. The level of the statistical significance was set at p< 0.05. Results: The results indicated that HDPSCs treated with AMTA and AMTA+Ze-Ag-Zn particles did not show any significant cell death compared with the control cells after 14 days of the treatment course while the ALP activity and calcium ion levels were significantly (p< 0.05) elevated. Also, the addition of AMTA particles to the cell culture media resulted in increased ALP activity and calcium ion level compared with HDPSCs treated with AMTA+ Ze-Ag-Zn particles on day 7 of the treatment course (p< 0.05). Conclusion: It seems that the incorporation of Ze-Ag-Zn particles into AMTA did not have any significant positive effect on the biomineralization properties of AMTA.https://dentjods.sums.ac.ir/article_47200_7c28f43e4f4da60c4edd8fd05c4e845f.pdfalkaline phosphatase; biomineralization; dental pulp stem cells; nanoparticles; zeolite; |
| spellingShingle | Negin Ghasemi Sadegh Salarinasab Reza Rahbarghazi Samin Sedghi Paria Davoudi Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells Journal of Dentistry alkaline phosphatase; biomineralization; dental pulp stem cells; nanoparticles; zeolite; |
| title | Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells |
| title_full | Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells |
| title_fullStr | Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells |
| title_full_unstemmed | Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells |
| title_short | Effect of Incorporation of Zeolite Containing Silver-Zinc Nanoparticles into Mineral Trioxide Aggregate on Odontogenic Activity of Human Dental Pulp Stem Cells |
| title_sort | effect of incorporation of zeolite containing silver zinc nanoparticles into mineral trioxide aggregate on odontogenic activity of human dental pulp stem cells |
| topic | alkaline phosphatase; biomineralization; dental pulp stem cells; nanoparticles; zeolite; |
| url | https://dentjods.sums.ac.ir/article_47200_7c28f43e4f4da60c4edd8fd05c4e845f.pdf |
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