In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges
In the last two decades, alginate scaffolds have been variously studied as extracellular matrix analogs for tissue engineering. However, relevant evidence is still lacking concerning their ability to mimic the microenvironment of hierarchical tissues such as bone. Hence, an increasing amount of atte...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00587/full |
| _version_ | 1818038608446095360 |
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| author | Janeth Serrano-Bello Iriczalli Cruz-Maya Iriczalli Cruz-Maya Fernando Suaste-Olmos Patricia González-Alva Rosaria Altobelli Luigi Ambrosio Luis Alberto Medina Luis Alberto Medina Vincenzo Guarino Marco Antonio Alvarez-Perez |
| author_facet | Janeth Serrano-Bello Iriczalli Cruz-Maya Iriczalli Cruz-Maya Fernando Suaste-Olmos Patricia González-Alva Rosaria Altobelli Luigi Ambrosio Luis Alberto Medina Luis Alberto Medina Vincenzo Guarino Marco Antonio Alvarez-Perez |
| author_sort | Janeth Serrano-Bello |
| collection | DOAJ |
| description | In the last two decades, alginate scaffolds have been variously studied as extracellular matrix analogs for tissue engineering. However, relevant evidence is still lacking concerning their ability to mimic the microenvironment of hierarchical tissues such as bone. Hence, an increasing amount of attention has recently been devoted to the fabrication of macro/microporous sponges with pore anisotropy able to more accurately replicate the cell niche structure as a trigger for bioactive functionalities. This paper presents an in vivo study of alginate sponges with anisotropic microporous domains (MAS) formed by ionic crosslinking in the presence of different fractions (30 or 50% v) of hydroxyapatite (HA). In comparison with unloaded sponges (MAS0), we demonstrated that HA confers peculiar physical and biological properties to the sponge, depending upon the inorganic fraction used, enabling the sponge to bio-mimetically support the regeneration of newly formed bone. Scanning electron microscopy analysis showed a preferential orientation of pores, ascribable to the physical constraints exerted by HA particles during the pore network formation. Energy dispersive spectroscopy (EDS) and X-Ray diffraction (XRD) confirmed a chemical affinity of HA with the native mineral phase of the bone. In vitro studies via WST-1 assay showed good adhesion and proliferation of human Dental Pulp-Mesenchymal Stem Cells (hDP-MSC) that increased in the presence of the bioactive HA signals. Moreover, in vivo studies via micro-CT and histological analyses of a bone model (e.g., a rat calvaria defect) confirmed that the maximum osteogenic response after 90 days was achieved with MAS30, which supported good regeneration of the calvaria defect without any evidence of inflammatory reaction. Hence, all of the results suggested that MAS is a promising scaffold for supporting the regeneration of hard tissues in different body compartments. |
| first_indexed | 2024-12-10T07:45:27Z |
| format | Article |
| id | doaj.art-162e552dc1bd475393af4090375c0c6d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-10T07:45:27Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-162e552dc1bd475393af4090375c0c6d2022-12-22T01:57:12ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-07-01810.3389/fbioe.2020.00587537097In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic SpongesJaneth Serrano-Bello0Iriczalli Cruz-Maya1Iriczalli Cruz-Maya2Fernando Suaste-Olmos3Patricia González-Alva4Rosaria Altobelli5Luigi Ambrosio6Luis Alberto Medina7Luis Alberto Medina8Vincenzo Guarino9Marco Antonio Alvarez-Perez10Tissue Bioengineering Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, MexicoTissue Bioengineering Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, MexicoInstitute of Polymers, Composites, and Biomaterials, National Research Council of Italy, Naples, ItalyInstituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, MexicoTissue Bioengineering Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, MexicoInstitute of Composite and Biomedical Materials, National Research Council of Italy, Naples, ItalyInstitute of Polymers, Composites, and Biomaterials, National Research Council of Italy, Naples, ItalyInstituto de Física, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología/Universidad Nacional Autónoma de México, Mexico City, MexicoInstitute of Polymers, Composites, and Biomaterials, National Research Council of Italy, Naples, ItalyTissue Bioengineering Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, MexicoIn the last two decades, alginate scaffolds have been variously studied as extracellular matrix analogs for tissue engineering. However, relevant evidence is still lacking concerning their ability to mimic the microenvironment of hierarchical tissues such as bone. Hence, an increasing amount of attention has recently been devoted to the fabrication of macro/microporous sponges with pore anisotropy able to more accurately replicate the cell niche structure as a trigger for bioactive functionalities. This paper presents an in vivo study of alginate sponges with anisotropic microporous domains (MAS) formed by ionic crosslinking in the presence of different fractions (30 or 50% v) of hydroxyapatite (HA). In comparison with unloaded sponges (MAS0), we demonstrated that HA confers peculiar physical and biological properties to the sponge, depending upon the inorganic fraction used, enabling the sponge to bio-mimetically support the regeneration of newly formed bone. Scanning electron microscopy analysis showed a preferential orientation of pores, ascribable to the physical constraints exerted by HA particles during the pore network formation. Energy dispersive spectroscopy (EDS) and X-Ray diffraction (XRD) confirmed a chemical affinity of HA with the native mineral phase of the bone. In vitro studies via WST-1 assay showed good adhesion and proliferation of human Dental Pulp-Mesenchymal Stem Cells (hDP-MSC) that increased in the presence of the bioactive HA signals. Moreover, in vivo studies via micro-CT and histological analyses of a bone model (e.g., a rat calvaria defect) confirmed that the maximum osteogenic response after 90 days was achieved with MAS30, which supported good regeneration of the calvaria defect without any evidence of inflammatory reaction. Hence, all of the results suggested that MAS is a promising scaffold for supporting the regeneration of hard tissues in different body compartments.https://www.frontiersin.org/article/10.3389/fbioe.2020.00587/fullalginatehydroxyapatiteanisotropic structuremicroporous spongeshard tissuesin vivo models |
| spellingShingle | Janeth Serrano-Bello Iriczalli Cruz-Maya Iriczalli Cruz-Maya Fernando Suaste-Olmos Patricia González-Alva Rosaria Altobelli Luigi Ambrosio Luis Alberto Medina Luis Alberto Medina Vincenzo Guarino Marco Antonio Alvarez-Perez In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges Frontiers in Bioengineering and Biotechnology alginate hydroxyapatite anisotropic structure microporous sponges hard tissues in vivo models |
| title | In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges |
| title_full | In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges |
| title_fullStr | In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges |
| title_full_unstemmed | In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges |
| title_short | In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges |
| title_sort | in vivo regeneration of mineralized bone tissue in anisotropic biomimetic sponges |
| topic | alginate hydroxyapatite anisotropic structure microporous sponges hard tissues in vivo models |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00587/full |
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