In vitro culture physiology
The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro plant development. However, growth rate, development and many of the physiologic-morphologic features of the in vitro grown plants are influenced by the culture vessel. The increa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2001-03-01
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| Series: | Biotecnología Vegetal |
| Subjects: | |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/59 |
| _version_ | 1818880900351393792 |
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| author | Maria Jesús Cañal Roberto Rodríguez Belén Fernández Ricardo Sánchez-Tames Juan Pedro Majada |
| author_facet | Maria Jesús Cañal Roberto Rodríguez Belén Fernández Ricardo Sánchez-Tames Juan Pedro Majada |
| author_sort | Maria Jesús Cañal |
| collection | DOAJ |
| description | The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro
plant development. However, growth rate, development and many of the physiologic-morphologic features of the in vitro
grown plants are influenced by the culture vessel. The increasing knowledge about the environmental control of culture
vessels under sterile conditions, is helping to change micorpropagation procedures. The in vitro environment with
lower rate ventilation, brings about low flow rates of matter and energy, with minimum variations of temperature, high
relative humidity and large daily changes of the concentration of CO 2 inside the culture vessel. The type of culture vessel
(size, shape, fabric and closing system) can influence the evolution of the atmosphere along the time of culture.
Although submitted to different stresses factors plant can be grown in vitro, but plants can be faulty in their anatomy,
morphology and physiology. As a consequence, these plants shown a phenotype unable to survive to ex vitro conditions.
Different strategies can be used to control the atmosphere along the different phases of micropropagation, in
heterotrophic, mixotrophic or autotrophic cultures. The election of the best strategy will be based on different factors as
species, number of transplantes required, or quality-price relationship. |
| first_indexed | 2024-12-19T14:53:19Z |
| format | Article |
| id | doaj.art-163196e01ad04c7a9e1f8871ad7c4e1d |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-19T14:53:19Z |
| publishDate | 2001-03-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-163196e01ad04c7a9e1f8871ad7c4e1d2022-12-21T20:16:47ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472001-03-01113948In vitro culture physiologyMaria Jesús Cañal0Roberto Rodríguez1 Belén Fernández 2 Ricardo Sánchez-Tames3Juan Pedro Majada4Lab. Fisiología Vegetal, Dpto B.O.S., C/ Catedrático Rodrígo Uría s/n 33071, Universidad de Oviedo, España.Lab. Fisiología Vegetal, Dpto B.O.S., C/ Catedrático Rodrígo Uría s/n 33071, Universidad de Oviedo, España.Lab. Fisiología Vegetal, Dpto B.O.S., C/ Catedrático Rodrígo Uría s/n 33071, Universidad de Oviedo, España.Lab. Fisiología Vegetal, Dpto B.O.S., C/ Catedrático Rodrígo Uría s/n 33071, Universidad de Oviedo, España.Lab. Fisiología Vegetal, Dpto B.O.S., C/ Catedrático Rodrígo Uría s/n 33071, Universidad de Oviedo, España.The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro plant development. However, growth rate, development and many of the physiologic-morphologic features of the in vitro grown plants are influenced by the culture vessel. The increasing knowledge about the environmental control of culture vessels under sterile conditions, is helping to change micorpropagation procedures. The in vitro environment with lower rate ventilation, brings about low flow rates of matter and energy, with minimum variations of temperature, high relative humidity and large daily changes of the concentration of CO 2 inside the culture vessel. The type of culture vessel (size, shape, fabric and closing system) can influence the evolution of the atmosphere along the time of culture. Although submitted to different stresses factors plant can be grown in vitro, but plants can be faulty in their anatomy, morphology and physiology. As a consequence, these plants shown a phenotype unable to survive to ex vitro conditions. Different strategies can be used to control the atmosphere along the different phases of micropropagation, in heterotrophic, mixotrophic or autotrophic cultures. The election of the best strategy will be based on different factors as species, number of transplantes required, or quality-price relationship.https://revista.ibp.co.cu/index.php/BV/article/view/59in vitro enviromentalcharacteristic physiologyenviromental controltissue culturemicropropagation |
| spellingShingle | Maria Jesús Cañal Roberto Rodríguez Belén Fernández Ricardo Sánchez-Tames Juan Pedro Majada In vitro culture physiology Biotecnología Vegetal in vitro enviromental characteristic physiology enviromental control tissue culture micropropagation |
| title | In vitro culture physiology |
| title_full | In vitro culture physiology |
| title_fullStr | In vitro culture physiology |
| title_full_unstemmed | In vitro culture physiology |
| title_short | In vitro culture physiology |
| title_sort | in vitro culture physiology |
| topic | in vitro enviromental characteristic physiology enviromental control tissue culture micropropagation |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/59 |
| work_keys_str_mv | AT mariajesuscanal invitroculturephysiology AT robertorodriguez invitroculturephysiology AT belenfernandez invitroculturephysiology AT ricardosancheztames invitroculturephysiology AT juanpedromajada invitroculturephysiology |