CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery.
MicroRNAs (miRNAs) are important regulators of gene expression, and their expression is associated with many physiological conditions. Here, we investigated potential associations between expression levels of miRNAs in human placenta and the onset of spontaneous term birth. Using RNA sequencing, we...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0234403 |
| _version_ | 1818716571870167040 |
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| author | Ravindra Daddali Marja Ojaniemi Mikko Hallman Mika Rämet Antti M Haapalainen |
| author_facet | Ravindra Daddali Marja Ojaniemi Mikko Hallman Mika Rämet Antti M Haapalainen |
| author_sort | Ravindra Daddali |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are important regulators of gene expression, and their expression is associated with many physiological conditions. Here, we investigated potential associations between expression levels of miRNAs in human placenta and the onset of spontaneous term birth. Using RNA sequencing, we identified 54 miRNAs differentially expressed during spontaneous term labor compared to elective term births. Expression levels of 23 miRNAs were upregulated, whereas 31 were downregulated at least 1.5-fold. The upregulated miRNA miR-371a-5p putatively targets CPPED1, expression of which decreases during spontaneous birth. We used a luciferase reporter-based assay to test whether a miR-371a-5p mimic affected translation when it bound to the 3' untranslated region of CPPED1. In this setting, the miR-371a-5p mimic resulted in lower luciferase activity, which suggests that miR-371a-5p regulates levels of CPPED1. In conclusion, inversely correlated levels of miR-371a-5p and CPPED1 suggest a role for both in spontaneous delivery. |
| first_indexed | 2024-12-17T19:21:23Z |
| format | Article |
| id | doaj.art-1631a2db7ab94968adf0fcccbe28a54f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T19:21:23Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1631a2db7ab94968adf0fcccbe28a54f2022-12-21T21:35:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01156e023440310.1371/journal.pone.0234403CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery.Ravindra DaddaliMarja OjaniemiMikko HallmanMika RämetAntti M HaapalainenMicroRNAs (miRNAs) are important regulators of gene expression, and their expression is associated with many physiological conditions. Here, we investigated potential associations between expression levels of miRNAs in human placenta and the onset of spontaneous term birth. Using RNA sequencing, we identified 54 miRNAs differentially expressed during spontaneous term labor compared to elective term births. Expression levels of 23 miRNAs were upregulated, whereas 31 were downregulated at least 1.5-fold. The upregulated miRNA miR-371a-5p putatively targets CPPED1, expression of which decreases during spontaneous birth. We used a luciferase reporter-based assay to test whether a miR-371a-5p mimic affected translation when it bound to the 3' untranslated region of CPPED1. In this setting, the miR-371a-5p mimic resulted in lower luciferase activity, which suggests that miR-371a-5p regulates levels of CPPED1. In conclusion, inversely correlated levels of miR-371a-5p and CPPED1 suggest a role for both in spontaneous delivery.https://doi.org/10.1371/journal.pone.0234403 |
| spellingShingle | Ravindra Daddali Marja Ojaniemi Mikko Hallman Mika Rämet Antti M Haapalainen CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. PLoS ONE |
| title | CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. |
| title_full | CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. |
| title_fullStr | CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. |
| title_full_unstemmed | CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. |
| title_short | CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery. |
| title_sort | cpped1 targeting microrna 371a 5p expression in human placenta associates with spontaneous delivery |
| url | https://doi.org/10.1371/journal.pone.0234403 |
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