First report of strawberry bacterial leaf blight caused by Pantoea ananatis in Egypt
Strawberry leaves showing leaf blight symptoms were collected from six different farms in Ismailia and Beheira Governorates in Egypt during the 2020–2021 growing season. Eight bacterial isolates, i.e., Pa1, Pa2, Pa3, Pa4 (Ismailia farms) and Pa5, Pa6, Pa7 and Pa8 (Beheira farms) were isolated. A...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences
2022-06-01
|
| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2022.141359 |
| Summary: | Strawberry leaves showing leaf blight symptoms were collected from six different farms
in Ismailia and Beheira Governorates in Egypt during the 2020–2021 growing season.
Eight bacterial isolates, i.e., Pa1, Pa2, Pa3, Pa4 (Ismailia farms) and Pa5, Pa6, Pa7 and Pa8
(Beheira farms) were isolated. A pathogenicity test of bacterial isolates was carried out
using detached strawberry leaf technique. All bacterial isolates produced leaf blight disease
symptoms. Isolates Pa2 and Pa6 showed the highest pathogenic characteristics with
clear symptoms on detached strawberry leaves. The phenotypic, biochemical and physiological
characters of the highest pathogenic isolates were confirmed by PCR analysis using
16S rRNA gene. The two bacterial isolates were identified as Pantoea ananatis with similarity
of 97.05% with accession number MH_127816.1 (isolate Pa2, Ismailia), while the
isolate (Pa6, Beheira) with similarity of 97.03% with accession number NR_026045.1. The
16S rDNA sequences were deposited in the GenBank nucleotide databases under accession
numbers OM258167 and OM279507, respectively. According to the pathogenicity
test, morphological and physiological characteristics as well as molecular data (16S rRNA
sequencing analysis), this finding is the first report of P. ananatis as a causal agent of strawberry
leaf blight disease in Egypt. |
|---|---|
| ISSN: | 1427-4345 1899-007X |