Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach
Abstract Background Cerebral ischemia is a serious neurological disorder that can lead to high morbidity and mortality. Chlorogenic acid is a polyphenol compound with antioxidant that can regulate proteins in cerebral ischemia. Middle cerebral artery occlusion (MCAO) surgery was performed to induce...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | Laboratory Animal Research |
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| Online Access: | https://doi.org/10.1186/s42826-023-00164-5 |
| _version_ | 1797806889690464256 |
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| author | Murad-Ali Shah Ju-Bin Kang Phil-Ok Koh |
| author_facet | Murad-Ali Shah Ju-Bin Kang Phil-Ok Koh |
| author_sort | Murad-Ali Shah |
| collection | DOAJ |
| description | Abstract Background Cerebral ischemia is a serious neurological disorder that can lead to high morbidity and mortality. Chlorogenic acid is a polyphenol compound with antioxidant that can regulate proteins in cerebral ischemia. Middle cerebral artery occlusion (MCAO) surgery was performed to induce ischemic brain injury and was maintained for 24 h. Chlorogenic acid (30 mg/kg) or vehicle was administrated into the peritoneal cavity 2 h after MCAO surgery. The cerebral cortical tissues were collected for further study and a proteomic approach was performed to identify the proteins changed by chlorogenic acid in the MCAO animals. Results We found that chlorogenic acid alleviated in changes in adenosylhomocysteinase, glycerol-3-phosphate dehydrogenase, eukaryotic translation initiation factor 4A-II, apolipoprotein A-I, and mu-crystallin. These proteins were reduced in MCAO animals with vehicle, and these reductions were attenuated by chlorogenic acid treatment. The mitigation of this reduction by chlorogenic acid was confirmed by the reverse transcription PCR technique. These proteins are associated with energy metabolism, protein synthesis, inflammation, and physiological metabolism. They are involved in the neuroprotective effect of chlorogenic acid. These results showed that chlorogenic acid alleviates the neurological disorders caused by MCAO and regulates the expression of proteins involved in neuroprotection. Conclusions Therefore, our findings provide evidence that chlorogenic acid plays a neuroprotective role in stroke animal models by controlling specific proteins. |
| first_indexed | 2024-03-13T06:14:14Z |
| format | Article |
| id | doaj.art-1634a48258ba4934afc0b11225e2c188 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-03-13T06:14:14Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-1634a48258ba4934afc0b11225e2c1882023-06-11T11:04:43ZengBMCLaboratory Animal Research2233-76602023-06-0139111210.1186/s42826-023-00164-5Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approachMurad-Ali Shah0Ju-Bin Kang1Phil-Ok Koh2Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National UniversityDepartment of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National UniversityDepartment of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National UniversityAbstract Background Cerebral ischemia is a serious neurological disorder that can lead to high morbidity and mortality. Chlorogenic acid is a polyphenol compound with antioxidant that can regulate proteins in cerebral ischemia. Middle cerebral artery occlusion (MCAO) surgery was performed to induce ischemic brain injury and was maintained for 24 h. Chlorogenic acid (30 mg/kg) or vehicle was administrated into the peritoneal cavity 2 h after MCAO surgery. The cerebral cortical tissues were collected for further study and a proteomic approach was performed to identify the proteins changed by chlorogenic acid in the MCAO animals. Results We found that chlorogenic acid alleviated in changes in adenosylhomocysteinase, glycerol-3-phosphate dehydrogenase, eukaryotic translation initiation factor 4A-II, apolipoprotein A-I, and mu-crystallin. These proteins were reduced in MCAO animals with vehicle, and these reductions were attenuated by chlorogenic acid treatment. The mitigation of this reduction by chlorogenic acid was confirmed by the reverse transcription PCR technique. These proteins are associated with energy metabolism, protein synthesis, inflammation, and physiological metabolism. They are involved in the neuroprotective effect of chlorogenic acid. These results showed that chlorogenic acid alleviates the neurological disorders caused by MCAO and regulates the expression of proteins involved in neuroprotection. Conclusions Therefore, our findings provide evidence that chlorogenic acid plays a neuroprotective role in stroke animal models by controlling specific proteins.https://doi.org/10.1186/s42826-023-00164-5Chlorogenic acidMiddle cerebral artery occlusionNeuroprotectionStroke |
| spellingShingle | Murad-Ali Shah Ju-Bin Kang Phil-Ok Koh Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach Laboratory Animal Research Chlorogenic acid Middle cerebral artery occlusion Neuroprotection Stroke |
| title | Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach |
| title_full | Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach |
| title_fullStr | Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach |
| title_full_unstemmed | Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach |
| title_short | Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach |
| title_sort | identification of proteins regulated by chlorogenic acid in an ischemic animal model a proteomic approach |
| topic | Chlorogenic acid Middle cerebral artery occlusion Neuroprotection Stroke |
| url | https://doi.org/10.1186/s42826-023-00164-5 |
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