Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico study
Objective(s): To investigate the effect of cinnamaldehyde and eugenol on the telomere-dependent senescence of stem cells. In addition, to search the probable targets of mentioned phytochemicals between human telomere interacting proteins (TIPs) using in silico studies. Materials and Methods: Human a...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2017-03-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_8362_70d7e96d3cd96ed5607f33aef067bab7.pdf |
| _version_ | 1818483707224260608 |
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| author | Abdorrahim Absalan Seyed Alireza Mesbah-Namin Taki Tiraihi Taher Taheri |
| author_facet | Abdorrahim Absalan Seyed Alireza Mesbah-Namin Taki Tiraihi Taher Taheri |
| author_sort | Abdorrahim Absalan |
| collection | DOAJ |
| description | Objective(s): To investigate the effect of cinnamaldehyde and eugenol on the telomere-dependent senescence of stem cells. In addition, to search the probable targets of mentioned phytochemicals between human telomere interacting proteins (TIPs) using in silico studies. Materials and Methods: Human adipose derived stem cells (hASCs) were studied under treatments with 2.5 µM/ml cinnamaldehyde, 0.1 µg/ml eugenol, 0.01% DMSO or any additive. The expression of TERT, AKT1 and DKC1 genes and the telomere length were assessed over 48-hr treatment. In addition, docking study was conducted to show probable ways through which phytochemicals interact with TIPs. Results: Treated and untreated hASCs had undetectable TERT expression, but they did affect the AKT1 and DKC1 expression levels (CI=0.95; P |
| first_indexed | 2024-12-10T15:45:29Z |
| format | Article |
| id | doaj.art-16370800d04f4558ad8dfdf6a2b7c74e |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-10T15:45:29Z |
| publishDate | 2017-03-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-16370800d04f4558ad8dfdf6a2b7c74e2022-12-22T01:42:57ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742017-03-0120331632610.22038/ijbms.2017.83628362Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico studyAbdorrahim Absalan0Seyed Alireza Mesbah-Namin1Taki Tiraihi2Taher Taheri3Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranShefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, IranObjective(s): To investigate the effect of cinnamaldehyde and eugenol on the telomere-dependent senescence of stem cells. In addition, to search the probable targets of mentioned phytochemicals between human telomere interacting proteins (TIPs) using in silico studies. Materials and Methods: Human adipose derived stem cells (hASCs) were studied under treatments with 2.5 µM/ml cinnamaldehyde, 0.1 µg/ml eugenol, 0.01% DMSO or any additive. The expression of TERT, AKT1 and DKC1 genes and the telomere length were assessed over 48-hr treatment. In addition, docking study was conducted to show probable ways through which phytochemicals interact with TIPs. Results: Treated and untreated hASCs had undetectable TERT expression, but they did affect the AKT1 and DKC1 expression levels (CI=0.95; Phttp://ijbms.mums.ac.ir/article_8362_70d7e96d3cd96ed5607f33aef067bab7.pdfAgingCinnamaldehydeEugenolStem cellsTelomeraseTelomere |
| spellingShingle | Abdorrahim Absalan Seyed Alireza Mesbah-Namin Taki Tiraihi Taher Taheri Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico study Iranian Journal of Basic Medical Sciences Aging Cinnamaldehyde Eugenol Stem cells Telomerase Telomere |
| title | Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico study |
| title_full | Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico study |
| title_fullStr | Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico study |
| title_full_unstemmed | Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico study |
| title_short | Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of human adipose-derived stem cells (hASCs): An experimental and in silico study |
| title_sort | cinnamaldehyde and eugenol change the expression folds of akt1 and dkc1 genes and decrease the telomere length of human adipose derived stem cells hascs an experimental and in silico study |
| topic | Aging Cinnamaldehyde Eugenol Stem cells Telomerase Telomere |
| url | http://ijbms.mums.ac.ir/article_8362_70d7e96d3cd96ed5607f33aef067bab7.pdf |
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