Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis
Abstract The butanol extract of Asparagus cochinchinensis roots fermented with Weissella cibaria (BAW) effectively prevents inflammation and remodeling of airway in the ovalbumin (OVA)-induced asthma model. To characterize biomarkers that can predict the anti-asthmatic effects induced by BAW treatme...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | Laboratory Animal Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42826-019-0033-x |
| _version_ | 1818726349095829504 |
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| author | Jun Young Choi So Hyun Kim Ji Eun Kim Ji Won Park Mi Ju Kang Hyeon Jun Choi Su Ji Bae Jae Ho Lee Young-Suk Jung Dae Youn Hwang |
| author_facet | Jun Young Choi So Hyun Kim Ji Eun Kim Ji Won Park Mi Ju Kang Hyeon Jun Choi Su Ji Bae Jae Ho Lee Young-Suk Jung Dae Youn Hwang |
| author_sort | Jun Young Choi |
| collection | DOAJ |
| description | Abstract The butanol extract of Asparagus cochinchinensis roots fermented with Weissella cibaria (BAW) effectively prevents inflammation and remodeling of airway in the ovalbumin (OVA)-induced asthma model. To characterize biomarkers that can predict the anti-asthmatic effects induced by BAW treatment, we measured the alteration of endogenous metabolites in the serum of OVA-induced asthma mice after administration of low concentration BAW (BAWLo, 250 mg/kg) and high concentration BAW (BAWHi, 500 mg/kg) using 1H nuclear magnetic resonance (1H-NMR) spectral data. The number of immune cells and serum concentration of IgE as well as thickness of the respiratory epithelium and infiltration of inflammatory cells in the airway significantly recovered in the OVA+BAW treated group as compared to the OVA+Vehicle treated group. In the metabolic profile analysis, the pattern recognition showed completely separate clustering of serum analysis parameters between the OVA+Vehicle and OVA+BAW treated groups. Of the total endogenous metabolites, 19 metabolites were upregulated or downregulated in the OVA+Vehicle treated group as compared to the Control treated group. However, only 4 amino acids (alanine, glycine, methionine and tryptophan) were significantly recovered after BAWLo and BAWHi treatment. This study provides the first results pertaining to metabolic changes in the asthma model mice treated with OVA+BAW. Additionally, these findings show that 4 metabolites can be used as one of biomarkers to predict the anti-asthmatic effects. |
| first_indexed | 2024-12-17T21:56:47Z |
| format | Article |
| id | doaj.art-1639c726d1414f50960a55cf3100836b |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-17T21:56:47Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-1639c726d1414f50960a55cf3100836b2022-12-21T21:31:05ZengBMCLaboratory Animal Research2233-76602019-12-0135111010.1186/s42826-019-0033-xFour amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensisJun Young Choi0So Hyun Kim1Ji Eun Kim2Ji Won Park3Mi Ju Kang4Hyeon Jun Choi5Su Ji Bae6Jae Ho Lee7Young-Suk Jung8Dae Youn Hwang9Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityCollege of Pharmacy, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityCollege of Pharmacy, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science, Pusan National UniversityAbstract The butanol extract of Asparagus cochinchinensis roots fermented with Weissella cibaria (BAW) effectively prevents inflammation and remodeling of airway in the ovalbumin (OVA)-induced asthma model. To characterize biomarkers that can predict the anti-asthmatic effects induced by BAW treatment, we measured the alteration of endogenous metabolites in the serum of OVA-induced asthma mice after administration of low concentration BAW (BAWLo, 250 mg/kg) and high concentration BAW (BAWHi, 500 mg/kg) using 1H nuclear magnetic resonance (1H-NMR) spectral data. The number of immune cells and serum concentration of IgE as well as thickness of the respiratory epithelium and infiltration of inflammatory cells in the airway significantly recovered in the OVA+BAW treated group as compared to the OVA+Vehicle treated group. In the metabolic profile analysis, the pattern recognition showed completely separate clustering of serum analysis parameters between the OVA+Vehicle and OVA+BAW treated groups. Of the total endogenous metabolites, 19 metabolites were upregulated or downregulated in the OVA+Vehicle treated group as compared to the Control treated group. However, only 4 amino acids (alanine, glycine, methionine and tryptophan) were significantly recovered after BAWLo and BAWHi treatment. This study provides the first results pertaining to metabolic changes in the asthma model mice treated with OVA+BAW. Additionally, these findings show that 4 metabolites can be used as one of biomarkers to predict the anti-asthmatic effects.https://doi.org/10.1186/s42826-019-0033-xAsparagus cochinchinensisFermentationWeissella cibariaAsthmaTherapeutic effectsMetabolomics |
| spellingShingle | Jun Young Choi So Hyun Kim Ji Eun Kim Ji Won Park Mi Ju Kang Hyeon Jun Choi Su Ji Bae Jae Ho Lee Young-Suk Jung Dae Youn Hwang Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis Laboratory Animal Research Asparagus cochinchinensis Fermentation Weissella cibaria Asthma Therapeutic effects Metabolomics |
| title | Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis |
| title_full | Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis |
| title_fullStr | Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis |
| title_full_unstemmed | Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis |
| title_short | Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis |
| title_sort | four amino acids as serum biomarkers for anti asthma effects in the ovalbumin induced asthma mouse model treated with extract of asparagus cochinchinensis |
| topic | Asparagus cochinchinensis Fermentation Weissella cibaria Asthma Therapeutic effects Metabolomics |
| url | https://doi.org/10.1186/s42826-019-0033-x |
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