A conditional counterselectable Piga knockout in mouse embryonic stem cells for advanced genome writing applications

A conditional counterselectable Piga knockout in mouse embryonic stem cells for advanced genome writing applications

Summary: Overwriting counterselectable markers is an efficient strategy for removing wild-type DNA or replacing it with payload DNA of interest. Currently, one bottleneck of efficient genome engineering in mammals is the shortage of counterselectable (negative selection) markers that work robustly w...

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Bibliographic Details
Main Authors: Weimin Zhang, Ran Brosh, Laura H. McCulloch, Yinan Zhu, Hannah Ashe, Gwen Ellis, Brendan R. Camellato, Sang Yong Kim, Matthew T. Maurano, Jef D. Boeke
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:iScience
Subjects:
Biotechnology
Genetic engineering
Bioengineering
Synthetic biology
Online Access:http://www.sciencedirect.com/science/article/pii/S258900422200709X

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http://www.sciencedirect.com/science/article/pii/S258900422200709X

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