High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses
ABSTRACT: The objective of this study was to determine Salmonella contamination levels, presence and serovar distribution in broiler carcasses before and after chilling, as well as to evaluate the effectiveness of chilling process. A total of 96 pooled neck skin samples (PNSS) of 48 prechill (PreC)...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001639 |
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| author | A. Demircioglu A.G. Coskun T.S. Kanar A. Eyigor S. Temelli |
| author_facet | A. Demircioglu A.G. Coskun T.S. Kanar A. Eyigor S. Temelli |
| author_sort | A. Demircioglu |
| collection | DOAJ |
| description | ABSTRACT: The objective of this study was to determine Salmonella contamination levels, presence and serovar distribution in broiler carcasses before and after chilling, as well as to evaluate the effectiveness of chilling process. A total of 96 pooled neck skin samples (PNSS) of 48 prechill (PreC) and 48 postchill (PosC) carcasses, representing 480 broilers collected in 6 mo’ period were analyzed using ISO 6579-2:2012 Miniaturized Most Probable Number (ISO-mMPN) technique. Species confirmation and serovar identification was performed by Salmonella-specific real-time PCR (Salm-PCR) and conventional serotyping, respectively. Mean Salmonella count was 1.84 log10 MPN/g in PreC, and 1.48 log10 MPN/g in PosC samples, indicating a statistically significant reduction of 0.36 log10 MPN/g (p < 0.05) in the counts by plant's air chill system. Salmonella positivity reduced from 97.9% (47/48) in PreC to 85.42% (41/48) in PosC samples, confirmed by Salm-PCR with identified serovars as S. Virchow (89.77 %) followed by S. Schwarzengrund (9.09%) and S. Bredeney (1.14%). Persistence of high load and prevalence of Salmonella with serovar Virchow dominance (other than the ones mandated in current guidelines) in the final product contributes significant and up to date data to relevant literature, and provides unbiased epidemiological reference to legal authorities for future relevant revisions. |
| first_indexed | 2024-04-25T00:16:17Z |
| format | Article |
| id | doaj.art-1641b0dedd354688b20375ec50e4bee2 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2025-03-22T03:29:01Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-1641b0dedd354688b20375ec50e4bee22024-04-29T04:14:03ZengElsevierPoultry Science0032-57912024-05-011035103584High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcassesA. Demircioglu0A.G. Coskun1T.S. Kanar2A. Eyigor3S. Temelli4Bursa Uludag University, Institute of Health Sciences, Department of Food Hygiene & Technology, Bursa, TurkiyeBursa Uludag University, Institute of Health Sciences, Department of Food Hygiene & Technology, Bursa, TurkiyeBursa Uludag University, Institute of Health Sciences, Department of Microbiology, Bursa, TurkiyeBursa Uludag University, Faculty of Veterinary Medicine, Department of Food Hygiene & Technology, Bursa, Turkiye; Corresponding author:Bursa Uludag University, Faculty of Veterinary Medicine, Department of Food Hygiene & Technology, Bursa, TurkiyeABSTRACT: The objective of this study was to determine Salmonella contamination levels, presence and serovar distribution in broiler carcasses before and after chilling, as well as to evaluate the effectiveness of chilling process. A total of 96 pooled neck skin samples (PNSS) of 48 prechill (PreC) and 48 postchill (PosC) carcasses, representing 480 broilers collected in 6 mo’ period were analyzed using ISO 6579-2:2012 Miniaturized Most Probable Number (ISO-mMPN) technique. Species confirmation and serovar identification was performed by Salmonella-specific real-time PCR (Salm-PCR) and conventional serotyping, respectively. Mean Salmonella count was 1.84 log10 MPN/g in PreC, and 1.48 log10 MPN/g in PosC samples, indicating a statistically significant reduction of 0.36 log10 MPN/g (p < 0.05) in the counts by plant's air chill system. Salmonella positivity reduced from 97.9% (47/48) in PreC to 85.42% (41/48) in PosC samples, confirmed by Salm-PCR with identified serovars as S. Virchow (89.77 %) followed by S. Schwarzengrund (9.09%) and S. Bredeney (1.14%). Persistence of high load and prevalence of Salmonella with serovar Virchow dominance (other than the ones mandated in current guidelines) in the final product contributes significant and up to date data to relevant literature, and provides unbiased epidemiological reference to legal authorities for future relevant revisions.http://www.sciencedirect.com/science/article/pii/S0032579124001639Salmonellaserovarbroilerenumerationreal-time PCRISO |
| spellingShingle | A. Demircioglu A.G. Coskun T.S. Kanar A. Eyigor S. Temelli High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses Poultry Science Salmonella serovar broiler enumeration real-time PCR ISO |
| title | High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses |
| title_full | High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses |
| title_fullStr | High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses |
| title_full_unstemmed | High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses |
| title_short | High Salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses |
| title_sort | high salmonella load with serovar virchow dominance pose major public safety risk in postchill broiler carcasses |
| topic | Salmonella serovar broiler enumeration real-time PCR ISO |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001639 |
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