Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans
Objective To study the effects of different high-dose X-ray on the expression of DNA damage repair related genes, the mortality and fertility in C.elegans. Methods Wild-type C.elegans were irradiated with 200 Gray (Gy) or 400 Gy of X-ray, then the expression of 14 DNA damage-repair related genes w...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-04-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200517.pdf |
| _version_ | 1827390567315668992 |
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| author | CHEN Li-na, LIU Zhi, ZHANG Chao |
| author_facet | CHEN Li-na, LIU Zhi, ZHANG Chao |
| author_sort | CHEN Li-na, LIU Zhi, ZHANG Chao |
| collection | DOAJ |
| description | Objective To study the effects of different high-dose X-ray on the expression of DNA damage repair related genes, the mortality and fertility in C.elegans. Methods Wild-type C.elegans were irradiated with 200 Gray (Gy) or 400 Gy of X-ray, then the expression of 14 DNA damage-repair related genes were quantified by RT-qPCR. STRING was adopted to analyze the protein-protein interaction of the differential genes.C.elegans survival was counted to assess the effect of X-ray on nematodes mortality and fertility. Results X-ray radiation caused abnormal expression of multiple DNA damage repair related genes in C.elegans as compared to the control group. Mortality of C.elegans increased(P＜0.05)and fecundity decreased(P＜0.05)after radiation. Conclusions X-ray radiation promotes apoptosis and inhibits fertility of C.elegans by changing the expression of multiple DNA damage and oxidation damage related genes. The age-1,cep-1,ced-4,rad-51 and daf-2 are sensitive to radiation, which are potential targets for radiotherapy. |
| first_indexed | 2024-03-08T16:53:38Z |
| format | Article |
| id | doaj.art-1642935a1b4740eb9b8209bb82d238b7 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:38Z |
| publishDate | 2021-04-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-1642935a1b4740eb9b8209bb82d238b72024-01-05T03:04:57ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-04-01414479483Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegansCHEN Li-na, LIU Zhi, ZHANG Chao01. Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Southern Medical University, Guangzhou 510515; ;2. Department of Ultrasound, Nanfang Hospital, Guangzhou 510000; ;3. Basic Medical College, Xiangnan University, Chenzhou 423000, ChinaObjective To study the effects of different high-dose X-ray on the expression of DNA damage repair related genes, the mortality and fertility in C.elegans. Methods Wild-type C.elegans were irradiated with 200 Gray (Gy) or 400 Gy of X-ray, then the expression of 14 DNA damage-repair related genes were quantified by RT-qPCR. STRING was adopted to analyze the protein-protein interaction of the differential genes.C.elegans survival was counted to assess the effect of X-ray on nematodes mortality and fertility. Results X-ray radiation caused abnormal expression of multiple DNA damage repair related genes in C.elegans as compared to the control group. Mortality of C.elegans increased(P＜0.05)and fecundity decreased(P＜0.05)after radiation. Conclusions X-ray radiation promotes apoptosis and inhibits fertility of C.elegans by changing the expression of multiple DNA damage and oxidation damage related genes. The age-1,cep-1,ced-4,rad-51 and daf-2 are sensitive to radiation, which are potential targets for radiotherapy.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200517.pdfionizing radiation|c.elegans|gene expression|apoptosis|fertility |
| spellingShingle | CHEN Li-na, LIU Zhi, ZHANG Chao Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans Jichu yixue yu linchuang ionizing radiation|c.elegans|gene expression|apoptosis|fertility |
| title | Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans |
| title_full | Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans |
| title_fullStr | Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans |
| title_full_unstemmed | Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans |
| title_short | Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans |
| title_sort | effects of x ray radiation on expression of dna damage repair related genes in c elegans |
| topic | ionizing radiation|c.elegans|gene expression|apoptosis|fertility |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200517.pdf |
| work_keys_str_mv | AT chenlinaliuzhizhangchao effectsofxrayradiationonexpressionofdnadamagerepairrelatedgenesincelegans |