The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L.
In this study, we investigated the impact of different land use intensities (LUI) on the root-associated microbiome of Dactylis glomerata (orchardgrass). For this purpose, eight sampling sites with different land use intensity levels but comparable soil properties were selected in the southwest of G...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00930/full |
| _version_ | 1819020588361973760 |
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| author | Jennifer Estendorfer Barbara Stempfhuber Paula Haury Gisle Vestergaard Matthias C. Rillig Jasmin Joshi Peter Schröder Michael Schloter Michael Schloter |
| author_facet | Jennifer Estendorfer Barbara Stempfhuber Paula Haury Gisle Vestergaard Matthias C. Rillig Jasmin Joshi Peter Schröder Michael Schloter Michael Schloter |
| author_sort | Jennifer Estendorfer |
| collection | DOAJ |
| description | In this study, we investigated the impact of different land use intensities (LUI) on the root-associated microbiome of Dactylis glomerata (orchardgrass). For this purpose, eight sampling sites with different land use intensity levels but comparable soil properties were selected in the southwest of Germany. Experimental plots covered land use levels from natural grassland up to intensively managed meadows. We used 16S rRNA gene based barcoding to assess the plant-associated community structure in the endosphere, rhizosphere and bulk soil of D. glomerata. Samples were taken at the reproductive stage of the plant in early summer. Our data indicated that roots harbor a distinct bacterial community, which clearly differed from the microbiome of the rhizosphere and bulk soil. Our results revealed Pseudomonadaceae, Enterobacteriaceae and Comamonadaceae as the most abundant endophytes independently of land use intensity. Rhizosphere and bulk soil were dominated also by Proteobacteria, but the most abundant families differed from those obtained from root samples. In the soil, the effect of land use intensity was more pronounced compared to root endophytes leading to a clearly distinct pattern of bacterial communities under different LUI from rhizosphere and bulk soil vs. endophytes. Overall, a change of community structure on the plant–soil interface was observed, as the number of shared OTUs between all three compartments investigated increased with decreasing land use intensity. Thus, our findings suggest a stronger interaction of the plant with its surrounding soil under low land use intensity. Furthermore, the amount and quality of available nitrogen was identified as a major driver for shifts in the microbiome structure in all compartments. |
| first_indexed | 2024-12-21T03:53:36Z |
| format | Article |
| id | doaj.art-16449ef1b4a94060902cef5863ea474a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T03:53:36Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-16449ef1b4a94060902cef5863ea474a2022-12-21T19:16:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.00930270822The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L.Jennifer Estendorfer0Barbara Stempfhuber1Paula Haury2Gisle Vestergaard3Matthias C. Rillig4Jasmin Joshi5Peter Schröder6Michael Schloter7Michael Schloter8Research Unit Comparative Microbiome Analysis, Helmholtz Zentrum MünchenNeuherberg, GermanyResearch Unit Comparative Microbiome Analysis, Helmholtz Zentrum MünchenNeuherberg, GermanyResearch Unit Comparative Microbiome Analysis, Helmholtz Zentrum MünchenNeuherberg, GermanyResearch Unit Comparative Microbiome Analysis, Helmholtz Zentrum MünchenNeuherberg, GermanyInstitute for Biology, Freie Universität BerlinBerlin, GermanyBiodiversity Research/Systematic Botany, Institute for Biochemistry und Biology, University of PotsdamPotsdam, GermanyResearch Unit Comparative Microbiome Analysis, Helmholtz Zentrum MünchenNeuherberg, GermanyResearch Unit Comparative Microbiome Analysis, Helmholtz Zentrum MünchenNeuherberg, GermanyChair of Soil Science, Technical University of MunichFreising, GermanyIn this study, we investigated the impact of different land use intensities (LUI) on the root-associated microbiome of Dactylis glomerata (orchardgrass). For this purpose, eight sampling sites with different land use intensity levels but comparable soil properties were selected in the southwest of Germany. Experimental plots covered land use levels from natural grassland up to intensively managed meadows. We used 16S rRNA gene based barcoding to assess the plant-associated community structure in the endosphere, rhizosphere and bulk soil of D. glomerata. Samples were taken at the reproductive stage of the plant in early summer. Our data indicated that roots harbor a distinct bacterial community, which clearly differed from the microbiome of the rhizosphere and bulk soil. Our results revealed Pseudomonadaceae, Enterobacteriaceae and Comamonadaceae as the most abundant endophytes independently of land use intensity. Rhizosphere and bulk soil were dominated also by Proteobacteria, but the most abundant families differed from those obtained from root samples. In the soil, the effect of land use intensity was more pronounced compared to root endophytes leading to a clearly distinct pattern of bacterial communities under different LUI from rhizosphere and bulk soil vs. endophytes. Overall, a change of community structure on the plant–soil interface was observed, as the number of shared OTUs between all three compartments investigated increased with decreasing land use intensity. Thus, our findings suggest a stronger interaction of the plant with its surrounding soil under low land use intensity. Furthermore, the amount and quality of available nitrogen was identified as a major driver for shifts in the microbiome structure in all compartments.http://journal.frontiersin.org/article/10.3389/fpls.2017.00930/fullDactylis glomerataland use changeendophytesrhizospheresoil microbiomebiodiversity |
| spellingShingle | Jennifer Estendorfer Barbara Stempfhuber Paula Haury Gisle Vestergaard Matthias C. Rillig Jasmin Joshi Peter Schröder Michael Schloter Michael Schloter The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L. Frontiers in Plant Science Dactylis glomerata land use change endophytes rhizosphere soil microbiome biodiversity |
| title | The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L. |
| title_full | The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L. |
| title_fullStr | The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L. |
| title_full_unstemmed | The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L. |
| title_short | The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L. |
| title_sort | influence of land use intensity on the plant associated microbiome of dactylis glomerata l |
| topic | Dactylis glomerata land use change endophytes rhizosphere soil microbiome biodiversity |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00930/full |
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