GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.

GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.

Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefi...

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Bibliographic Details
Main Authors: Alejandro Sarrion-Perdigones, Erica Elvira Falconi, Sara I Zandalinas, Paloma Juárez, Asun Fernández-del-Carmen, Antonio Granell, Diego Orzaez
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21750718/pdf/?tool=EBI

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