Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian Cancers
The receptor activator of nuclear factor kappa B (RANK) is becoming recognized as a master regulator of tumorigenesis, yet its role in gynecological cancers remains mostly unexplored. We investigated whether there is a gradation of RANK protein and mRNA expression in epithelial ovarian cancer (EOC)...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1742 |
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| author | Raul Gomez Miguel Á. Tejada Víctor Rodríguez-García Octavio Burgués Ana I. Santos-Llamas Andrea Martínez-Massa Antonio Marín-Montes Juan J. Tarín Antonio Cano |
| author_facet | Raul Gomez Miguel Á. Tejada Víctor Rodríguez-García Octavio Burgués Ana I. Santos-Llamas Andrea Martínez-Massa Antonio Marín-Montes Juan J. Tarín Antonio Cano |
| author_sort | Raul Gomez |
| collection | DOAJ |
| description | The receptor activator of nuclear factor kappa B (RANK) is becoming recognized as a master regulator of tumorigenesis, yet its role in gynecological cancers remains mostly unexplored. We investigated whether there is a gradation of RANK protein and mRNA expression in epithelial ovarian cancer (EOC) according to malignancy and tumor staging. Immunohistochemical expression of RANK was examined in a cohort of 135 (benign <i>n</i> = 29, borderline <i>n</i>= 23 and malignant <i>n</i> = 83) EOCs. Wild type and truncated RANK mRNA isoform quantification was performed in a cohort of 168 (benign <i>n</i> = 26, borderline <i>n</i> = 13 and malignant <i>n</i> = 129) EOCs. RANK protein and mRNA values were increased in malignant vs. benign or borderline conditions across serous, mucinous and endometrioid cancer subtypes. Additionally, a trend of increased RANK values with staging was observed for the mucinous and serous histotype. Thus, increased expression of RANK appears associated with the evolution of disease to the onset of malignancy in EOC. Moreover, in some EOC histotypes, RANK expression is additionally associated with clinicopathological markers of tumor aggressiveness, suggesting a role in further progression of tumor activity. |
| first_indexed | 2024-03-09T23:44:30Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:44:30Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-164be5e6635d4c23866b1da38c282cb72023-11-23T16:45:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01233174210.3390/ijms23031742Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian CancersRaul Gomez0Miguel Á. Tejada1Víctor Rodríguez-García2Octavio Burgués3Ana I. Santos-Llamas4Andrea Martínez-Massa5Antonio Marín-Montes6Juan J. Tarín7Antonio Cano8Research Unit on Women’s Health-Institute of Health Research, INCLIVA, 46010 Valencia, SpainResearch Unit on Women’s Health-Institute of Health Research, INCLIVA, 46010 Valencia, SpainDepartment of Pediatrics and Obstetrics and Gynecology, University of Valencia, 46010 Valencia, SpainDepartment of Pathology, Hospital Clinico Universitario, 46010 Valencia, SpainResearch Unit on Women’s Health-Institute of Health Research, INCLIVA, 46010 Valencia, SpainService of Obstetrics and Gynecology, Hospital Clínico Universitario, Av Blasco Ibáñez 17, 46010 Valencia, SpainService of Obstetrics and Gynecology, Hospital Clínico Universitario, Av Blasco Ibáñez 17, 46010 Valencia, SpainResearch Unit on Women’s Health-Institute of Health Research, INCLIVA, 46010 Valencia, SpainResearch Unit on Women’s Health-Institute of Health Research, INCLIVA, 46010 Valencia, SpainThe receptor activator of nuclear factor kappa B (RANK) is becoming recognized as a master regulator of tumorigenesis, yet its role in gynecological cancers remains mostly unexplored. We investigated whether there is a gradation of RANK protein and mRNA expression in epithelial ovarian cancer (EOC) according to malignancy and tumor staging. Immunohistochemical expression of RANK was examined in a cohort of 135 (benign <i>n</i> = 29, borderline <i>n</i>= 23 and malignant <i>n</i> = 83) EOCs. Wild type and truncated RANK mRNA isoform quantification was performed in a cohort of 168 (benign <i>n</i> = 26, borderline <i>n</i> = 13 and malignant <i>n</i> = 129) EOCs. RANK protein and mRNA values were increased in malignant vs. benign or borderline conditions across serous, mucinous and endometrioid cancer subtypes. Additionally, a trend of increased RANK values with staging was observed for the mucinous and serous histotype. Thus, increased expression of RANK appears associated with the evolution of disease to the onset of malignancy in EOC. Moreover, in some EOC histotypes, RANK expression is additionally associated with clinicopathological markers of tumor aggressiveness, suggesting a role in further progression of tumor activity.https://www.mdpi.com/1422-0067/23/3/1742epithelial ovarian cancersRANKimmunohistochemistrytumor stage, tumor grade |
| spellingShingle | Raul Gomez Miguel Á. Tejada Víctor Rodríguez-García Octavio Burgués Ana I. Santos-Llamas Andrea Martínez-Massa Antonio Marín-Montes Juan J. Tarín Antonio Cano Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian Cancers International Journal of Molecular Sciences epithelial ovarian cancers RANK immunohistochemistry tumor stage, tumor grade |
| title | Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian Cancers |
| title_full | Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian Cancers |
| title_fullStr | Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian Cancers |
| title_full_unstemmed | Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian Cancers |
| title_short | Histological Grade and Tumor Stage Are Correlated with Expression of Receptor Activator of Nuclear Factor Kappa b (Rank) in Epithelial Ovarian Cancers |
| title_sort | histological grade and tumor stage are correlated with expression of receptor activator of nuclear factor kappa b rank in epithelial ovarian cancers |
| topic | epithelial ovarian cancers RANK immunohistochemistry tumor stage, tumor grade |
| url | https://www.mdpi.com/1422-0067/23/3/1742 |
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