Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and Plastids
Plant NADPH-dependent glyoxylate/succinic semialdehyde reductases 1 and 2 (GLYR1 and GLYR2) are considered to be involved in detoxifying harmful aldehydes, thereby preserving plant health during exposure to various abiotic stresses. Phylogenetic analysis revealed that the two GLYR isoforms appeared...
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| Format: | Article |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00601/full |
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| author | Barry J. Shelp Carolyne J. Brikis Adel Zarei Christopher P. Trobacher Jennifer R. DeEll Kazuhito Akama Robert T. Mullen Gale G. Bozzo |
| author_facet | Barry J. Shelp Carolyne J. Brikis Adel Zarei Christopher P. Trobacher Jennifer R. DeEll Kazuhito Akama Robert T. Mullen Gale G. Bozzo |
| author_sort | Barry J. Shelp |
| collection | DOAJ |
| description | Plant NADPH-dependent glyoxylate/succinic semialdehyde reductases 1 and 2 (GLYR1 and GLYR2) are considered to be involved in detoxifying harmful aldehydes, thereby preserving plant health during exposure to various abiotic stresses. Phylogenetic analysis revealed that the two GLYR isoforms appeared in the plant lineage prior to the divergence of the Chlorophyta and Streptophyta, which occurred approximately 750 million years ago. Green fluorescent protein fusions of apple (Malus x domestica Borkh.), rice (Oryza sativa L.) and Arabidopsis thaliana [L.] Heynh GLYRs were transiently expressed in tobacco (Nicotiana tabaccum L.) suspension cells or Arabidopsis protoplasts, as well in methoxyfenozide-induced, stably transformed Arabidopsis seedlings. The localization of apple GLYR1 confirmed that this isoform is cytosolic, whereas apple, rice and Arabidopsis GLYR2s were localized to both mitochondria and plastids. These findings highlight the potential involvement of GLYRs within distinct compartments of the plant cell. |
| first_indexed | 2024-12-22T13:16:38Z |
| format | Article |
| id | doaj.art-164c8c838ac9426488e201e787bc3720 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T13:16:38Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-164c8c838ac9426488e201e787bc37202022-12-21T18:24:35ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-04-01810.3389/fpls.2017.00601247742Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and PlastidsBarry J. Shelp0Carolyne J. Brikis1Adel Zarei2Christopher P. Trobacher3Jennifer R. DeEll4Kazuhito Akama5Robert T. Mullen6Gale G. Bozzo7Department of Plant Agriculture, University of Guelph, GuelphON, CanadaDepartment of Plant Agriculture, University of Guelph, GuelphON, CanadaDepartment of Plant Agriculture, University of Guelph, GuelphON, CanadaDepartment of Plant Agriculture, University of Guelph, GuelphON, CanadaOntario Ministry of Agriculture Food and Rural Affairs, SimcoeON, CanadaDepartment of Biological Science, Shimane UniversityMatsue, JapanDepartment of Molecular and Cellular Biology, University of Guelph, GuelphON, CanadaDepartment of Plant Agriculture, University of Guelph, GuelphON, CanadaPlant NADPH-dependent glyoxylate/succinic semialdehyde reductases 1 and 2 (GLYR1 and GLYR2) are considered to be involved in detoxifying harmful aldehydes, thereby preserving plant health during exposure to various abiotic stresses. Phylogenetic analysis revealed that the two GLYR isoforms appeared in the plant lineage prior to the divergence of the Chlorophyta and Streptophyta, which occurred approximately 750 million years ago. Green fluorescent protein fusions of apple (Malus x domestica Borkh.), rice (Oryza sativa L.) and Arabidopsis thaliana [L.] Heynh GLYRs were transiently expressed in tobacco (Nicotiana tabaccum L.) suspension cells or Arabidopsis protoplasts, as well in methoxyfenozide-induced, stably transformed Arabidopsis seedlings. The localization of apple GLYR1 confirmed that this isoform is cytosolic, whereas apple, rice and Arabidopsis GLYR2s were localized to both mitochondria and plastids. These findings highlight the potential involvement of GLYRs within distinct compartments of the plant cell.http://journal.frontiersin.org/article/10.3389/fpls.2017.00601/fullabiotic stressγ-aminobutyrateglyoxylate/succinic semialdehyde reductasephotorespirationphylogenetic analysissubcellular localization |
| spellingShingle | Barry J. Shelp Carolyne J. Brikis Adel Zarei Christopher P. Trobacher Jennifer R. DeEll Kazuhito Akama Robert T. Mullen Gale G. Bozzo Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and Plastids Frontiers in Plant Science abiotic stress γ-aminobutyrate glyoxylate/succinic semialdehyde reductase photorespiration phylogenetic analysis subcellular localization |
| title | Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and Plastids |
| title_full | Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and Plastids |
| title_fullStr | Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and Plastids |
| title_full_unstemmed | Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and Plastids |
| title_short | Ancient Plant Glyoxylate/Succinic Semialdehyde Reductases: GLYR1s Are Cytosolic, Whereas GLYR2s Are Localized to Both Mitochondria and Plastids |
| title_sort | ancient plant glyoxylate succinic semialdehyde reductases glyr1s are cytosolic whereas glyr2s are localized to both mitochondria and plastids |
| topic | abiotic stress γ-aminobutyrate glyoxylate/succinic semialdehyde reductase photorespiration phylogenetic analysis subcellular localization |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00601/full |
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