Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain
Abstract Background Effective vaccines against porcine reproductive and respiratory syndrome virus (PRRSV), especially against highly pathogenic (HP) PRRSV are still missing. The objective of this study was to evaluate the protective efficacy of an experimental live attenuated PRRSV 2 vaccine, compo...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-04-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1451-y |
| _version_ | 1818284890080149504 |
|---|---|
| author | Tatjana Sattler Jutta Pikalo Eveline Wodak Sandra Revilla-Fernández Adi Steinrigl Zoltán Bagó Ferdinand Entenfellner Jean-Baptiste Claude Floriane Pez Maela Francillette Friedrich Schmoll |
| author_facet | Tatjana Sattler Jutta Pikalo Eveline Wodak Sandra Revilla-Fernández Adi Steinrigl Zoltán Bagó Ferdinand Entenfellner Jean-Baptiste Claude Floriane Pez Maela Francillette Friedrich Schmoll |
| author_sort | Tatjana Sattler |
| collection | DOAJ |
| description | Abstract Background Effective vaccines against porcine reproductive and respiratory syndrome virus (PRRSV), especially against highly pathogenic (HP) PRRSV are still missing. The objective of this study was to evaluate the protective efficacy of an experimental live attenuated PRRSV 2 vaccine, composed of two strains, against heterologous challenge with a Vietnamese HP PRRSV 2 field strain. For this reason, 20 PRRSV negative piglets were divided into two groups. The pigs of group 1 were vaccinated with the experimental vaccine, group 2 remained unvaccinated. All study piglets received an intranasal challenge of the HP PRRSV 2 on day 0 of the study (42 days after vaccination). Blood samples were taken on days 7 and 21 after vaccination and on several days after challenge. On day 28 after challenge, all piglets were euthanized and pathologically examined. Results On days 7 and 21 after vaccination, a PRRSV 2 viraemia was seen in all piglets of group 1 which remained detectable in seven piglets up to 42 days after vaccination. On day 3 after challenge, all piglets from both groups were positive in PRRSV 2 RT-qPCR. From day 7 onwards, viral load and number of PRRSV 2 positive pigs were lower in group 1 than in group 2. All pigs of group 1 seroconverted after PRRSV 2 vaccination. PRRSV antibodies were detected in serum of all study pigs from both groups from day 14 after challenge onwards. In group 2, moderate respiratory symptoms with occasional coughing were seen following the challenge with HP PRRSV 2. Pigs of group 1 remained clinically unaffected. Interstitial pneumonia was found in four piglets of group 1 and in all ten piglets of group 2. Histopathological findings were more severe in group 2. Conclusions It was thus concluded that the used PRRSV 2 live experimental vaccine provided protection from clinical disease and marked reduction of histopathological findings and viral load in pigs challenged with a Vietnamese HP PRRSV 2 field strain. |
| first_indexed | 2024-12-13T00:59:59Z |
| format | Article |
| id | doaj.art-164ea7f6867d424389ff1511de9ba1ce |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-13T00:59:59Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-164ea7f6867d424389ff1511de9ba1ce2022-12-22T00:04:42ZengBMCBMC Veterinary Research1746-61482018-04-0114111010.1186/s12917-018-1451-yEfficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strainTatjana Sattler0Jutta Pikalo1Eveline Wodak2Sandra Revilla-Fernández3Adi Steinrigl4Zoltán Bagó5Ferdinand Entenfellner6Jean-Baptiste Claude7Floriane Pez8Maela Francillette9Friedrich Schmoll10Institute for Veterinary Disease Control, AGESInstitute for Veterinary Disease Control, AGESInstitute for Veterinary Disease Control, AGESInstitute for Veterinary Disease Control, AGESInstitute for Veterinary Disease Control, AGESInstitute for Veterinary Disease Control, AGESVeterinary Practice EntenfellnerBioSellal, Bâtiment AccinovBioSellal, Bâtiment AccinovBioSellal, Bâtiment AccinovInstitute for Veterinary Disease Control, AGESAbstract Background Effective vaccines against porcine reproductive and respiratory syndrome virus (PRRSV), especially against highly pathogenic (HP) PRRSV are still missing. The objective of this study was to evaluate the protective efficacy of an experimental live attenuated PRRSV 2 vaccine, composed of two strains, against heterologous challenge with a Vietnamese HP PRRSV 2 field strain. For this reason, 20 PRRSV negative piglets were divided into two groups. The pigs of group 1 were vaccinated with the experimental vaccine, group 2 remained unvaccinated. All study piglets received an intranasal challenge of the HP PRRSV 2 on day 0 of the study (42 days after vaccination). Blood samples were taken on days 7 and 21 after vaccination and on several days after challenge. On day 28 after challenge, all piglets were euthanized and pathologically examined. Results On days 7 and 21 after vaccination, a PRRSV 2 viraemia was seen in all piglets of group 1 which remained detectable in seven piglets up to 42 days after vaccination. On day 3 after challenge, all piglets from both groups were positive in PRRSV 2 RT-qPCR. From day 7 onwards, viral load and number of PRRSV 2 positive pigs were lower in group 1 than in group 2. All pigs of group 1 seroconverted after PRRSV 2 vaccination. PRRSV antibodies were detected in serum of all study pigs from both groups from day 14 after challenge onwards. In group 2, moderate respiratory symptoms with occasional coughing were seen following the challenge with HP PRRSV 2. Pigs of group 1 remained clinically unaffected. Interstitial pneumonia was found in four piglets of group 1 and in all ten piglets of group 2. Histopathological findings were more severe in group 2. Conclusions It was thus concluded that the used PRRSV 2 live experimental vaccine provided protection from clinical disease and marked reduction of histopathological findings and viral load in pigs challenged with a Vietnamese HP PRRSV 2 field strain.http://link.springer.com/article/10.1186/s12917-018-1451-yHP PRRSV 2VaccineEfficacyViral replicationImmune response |
| spellingShingle | Tatjana Sattler Jutta Pikalo Eveline Wodak Sandra Revilla-Fernández Adi Steinrigl Zoltán Bagó Ferdinand Entenfellner Jean-Baptiste Claude Floriane Pez Maela Francillette Friedrich Schmoll Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain BMC Veterinary Research HP PRRSV 2 Vaccine Efficacy Viral replication Immune response |
| title | Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain |
| title_full | Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain |
| title_fullStr | Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain |
| title_full_unstemmed | Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain |
| title_short | Efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous Vietnamese PRRSV 2 field strain |
| title_sort | efficacy of live attenuated porcine reproductive and respiratory syndrome virus 2 strains to protect pigs from challenge with a heterologous vietnamese prrsv 2 field strain |
| topic | HP PRRSV 2 Vaccine Efficacy Viral replication Immune response |
| url | http://link.springer.com/article/10.1186/s12917-018-1451-y |
| work_keys_str_mv | AT tatjanasattler efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT juttapikalo efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT evelinewodak efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT sandrarevillafernandez efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT adisteinrigl efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT zoltanbago efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT ferdinandentenfellner efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT jeanbaptisteclaude efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT florianepez efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT maelafrancillette efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain AT friedrichschmoll efficacyofliveattenuatedporcinereproductiveandrespiratorysyndromevirus2strainstoprotectpigsfromchallengewithaheterologousvietnameseprrsv2fieldstrain |