Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats
Context Berberine (Ber) can increase the survival rate of septic mice and inhibit inflammation, but whether it has a protective effect on septic cardiomyopathy (SCM) is unclear. Objective To investigate whether Ber ameliorates SCM in a rat model and its potential mechanism. Materials and methods Mal...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | http://dx.doi.org/10.1080/13880209.2021.1877736 |
| _version_ | 1818359960603459584 |
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| author | Huiqi Chen Qian Liu Xiangqi Liu Jinlan Jin |
| author_facet | Huiqi Chen Qian Liu Xiangqi Liu Jinlan Jin |
| author_sort | Huiqi Chen |
| collection | DOAJ |
| description | Context Berberine (Ber) can increase the survival rate of septic mice and inhibit inflammation, but whether it has a protective effect on septic cardiomyopathy (SCM) is unclear. Objective To investigate whether Ber ameliorates SCM in a rat model and its potential mechanism. Materials and methods Male SD rats were randomly divided into three groups: control (Con, n = 6) (DD H2O, 2 mL/100 g, ig, qd × 3 d, then saline, 10 mg/kg, ip); sepsis [LPS (lipopolysaccharide), n = 18] (LPS 10 mg/kg instead of saline, ip); and berberine intervention (Ber, n = 18) (Ber, 50 mg/kg instead of DD H2O, ig, qd × 3 d, LPS instead of saline, ip). Hemodynamics, HE staining, ELISA and western blot were performed at 6, 24, and 48 h after intraperitoneal injection of LPS to evaluate the effect of berberine in septic rats. Result Berberine could recover myocardial injury by partially increased ± dp/dt max (1151, 445 mmHg/s) and LVEDP levels (1.49 mmHg) with LPS-induced rats, as well as an ameliorated increase of cTnT (217.53 pg/mL) in the Ber group compared with that in the LPS group (at 24 h). In addition, HE staining results showed that berberine attenuated the myocardial cell swelling induced by LPS. In contrast to the LPS group, the up-regulation of TLR4, p65 TNF-α, and IL-1β were attenuated in the Ber group. Discussion and conclusions Berberine showed a protective effect on septic cardiomyopathy rats possibly through inhibiting the activation of TLR4/NF-κB signalling pathway. Whether it improves SCM through other mechanisms is our ongoing research. |
| first_indexed | 2024-12-13T20:53:12Z |
| format | Article |
| id | doaj.art-165542ce67c54ec597cfe72858574ab0 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-13T20:53:12Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-165542ce67c54ec597cfe72858574ab02022-12-21T23:31:49ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162021-01-0159112112810.1080/13880209.2021.18777361877736Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in ratsHuiqi Chen0Qian Liu1Xiangqi Liu2Jinlan Jin3Department of Ultrasonography, Guangzhou Red Cross Hospital, Medical College, Jinan UniversityDepartment of Cardiology, The Second Affiliated Hospital, University of SouthDepartment of Critical Care Medicine, Shenzhen Hospital (Futian) of Guangzhou University of Chinese MedicineDepartment of Critical Care Medicine, Shenzhen Hospital (Futian) of Guangzhou University of Chinese MedicineContext Berberine (Ber) can increase the survival rate of septic mice and inhibit inflammation, but whether it has a protective effect on septic cardiomyopathy (SCM) is unclear. Objective To investigate whether Ber ameliorates SCM in a rat model and its potential mechanism. Materials and methods Male SD rats were randomly divided into three groups: control (Con, n = 6) (DD H2O, 2 mL/100 g, ig, qd × 3 d, then saline, 10 mg/kg, ip); sepsis [LPS (lipopolysaccharide), n = 18] (LPS 10 mg/kg instead of saline, ip); and berberine intervention (Ber, n = 18) (Ber, 50 mg/kg instead of DD H2O, ig, qd × 3 d, LPS instead of saline, ip). Hemodynamics, HE staining, ELISA and western blot were performed at 6, 24, and 48 h after intraperitoneal injection of LPS to evaluate the effect of berberine in septic rats. Result Berberine could recover myocardial injury by partially increased ± dp/dt max (1151, 445 mmHg/s) and LVEDP levels (1.49 mmHg) with LPS-induced rats, as well as an ameliorated increase of cTnT (217.53 pg/mL) in the Ber group compared with that in the LPS group (at 24 h). In addition, HE staining results showed that berberine attenuated the myocardial cell swelling induced by LPS. In contrast to the LPS group, the up-regulation of TLR4, p65 TNF-α, and IL-1β were attenuated in the Ber group. Discussion and conclusions Berberine showed a protective effect on septic cardiomyopathy rats possibly through inhibiting the activation of TLR4/NF-κB signalling pathway. Whether it improves SCM through other mechanisms is our ongoing research.http://dx.doi.org/10.1080/13880209.2021.1877736sepsishemodynamicsp65myocardial dysfunctiontraditional chinese medicine |
| spellingShingle | Huiqi Chen Qian Liu Xiangqi Liu Jinlan Jin Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats Pharmaceutical Biology sepsis hemodynamics p65 myocardial dysfunction traditional chinese medicine |
| title | Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats |
| title_full | Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats |
| title_fullStr | Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats |
| title_full_unstemmed | Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats |
| title_short | Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats |
| title_sort | berberine attenuates septic cardiomyopathy by inhibiting tlr4 nf κb signalling in rats |
| topic | sepsis hemodynamics p65 myocardial dysfunction traditional chinese medicine |
| url | http://dx.doi.org/10.1080/13880209.2021.1877736 |
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