The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term Freezing
The preadaptation of probiotics to sub-lethal levels of multiple stress factors boosts their survival and stability. However, little is known about how long-term cold storage affects the properties of such preadapted probiotics. This study examined the impact of long-term freezing on structural and...
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MDPI AG
2022-11-01
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| Series: | Microbiology Research |
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| Online Access: | https://www.mdpi.com/2036-7481/13/4/64 |
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| author | Thobeka N. Dlangalala Moloko G. Mathipa-Mdakane Mapitsi S. Thantsha |
| author_facet | Thobeka N. Dlangalala Moloko G. Mathipa-Mdakane Mapitsi S. Thantsha |
| author_sort | Thobeka N. Dlangalala |
| collection | DOAJ |
| description | The preadaptation of probiotics to sub-lethal levels of multiple stress factors boosts their survival and stability. However, little is known about how long-term cold storage affects the properties of such preadapted probiotics. This study examined the impact of long-term freezing on structural and functional properties of multi-stress (acid, bile and heat) adapted <i>Lactiplantibacillus plantarum</i> B411. Cell morphology was investigated using scanning electron microscopy, and then their selected functional (bile salt hydrolase (BSH) activity, surface hydrophobicity, auto-aggregative and antimicrobial) properties were evaluated. Furthermore, the survival of <i>L. plantarum</i> B411 cells in yoghurt and juices during storage and under simulated gastrointestinal (GIT) conditions was evaluated. Long-term freezing negatively affected the morphology, auto-aggregation ability, BSH and antimicrobial activities of <i>L. plantarum</i> B411. The viability of freshly adapted and old adapted <i>L. plantarum</i> B411 cells in foods was similar. Under simulated GIT conditions, the viability of the stress adapted cells from the freezer diminished more than that of freshly adapted cells. Prolonged freezing compromised some functional properties of stress adapted cells and their stability under simulated GIT conditions. Care should thus be taken to ensure that a method used to preserve stress adapted cells does not cause them to lose beneficial properties, nor revert to their pre-adaptation status. |
| first_indexed | 2024-03-09T16:04:50Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2036-7481 |
| language | English |
| last_indexed | 2024-03-09T16:04:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-165693bb85454e7d9089a93e1b512f9e2023-11-24T16:42:51ZengMDPI AGMicrobiology Research2036-74812022-11-0113490992710.3390/microbiolres13040064The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term FreezingThobeka N. Dlangalala0Moloko G. Mathipa-Mdakane1Mapitsi S. Thantsha2Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0002, South AfricaDepartment of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0002, South AfricaDepartment of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0002, South AfricaThe preadaptation of probiotics to sub-lethal levels of multiple stress factors boosts their survival and stability. However, little is known about how long-term cold storage affects the properties of such preadapted probiotics. This study examined the impact of long-term freezing on structural and functional properties of multi-stress (acid, bile and heat) adapted <i>Lactiplantibacillus plantarum</i> B411. Cell morphology was investigated using scanning electron microscopy, and then their selected functional (bile salt hydrolase (BSH) activity, surface hydrophobicity, auto-aggregative and antimicrobial) properties were evaluated. Furthermore, the survival of <i>L. plantarum</i> B411 cells in yoghurt and juices during storage and under simulated gastrointestinal (GIT) conditions was evaluated. Long-term freezing negatively affected the morphology, auto-aggregation ability, BSH and antimicrobial activities of <i>L. plantarum</i> B411. The viability of freshly adapted and old adapted <i>L. plantarum</i> B411 cells in foods was similar. Under simulated GIT conditions, the viability of the stress adapted cells from the freezer diminished more than that of freshly adapted cells. Prolonged freezing compromised some functional properties of stress adapted cells and their stability under simulated GIT conditions. Care should thus be taken to ensure that a method used to preserve stress adapted cells does not cause them to lose beneficial properties, nor revert to their pre-adaptation status.https://www.mdpi.com/2036-7481/13/4/64<i>Lactiplantibacillus plantarum</i>stresspreadaptationprobioticsfreezing |
| spellingShingle | Thobeka N. Dlangalala Moloko G. Mathipa-Mdakane Mapitsi S. Thantsha The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term Freezing Microbiology Research <i>Lactiplantibacillus plantarum</i> stress preadaptation probiotics freezing |
| title | The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term Freezing |
| title_full | The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term Freezing |
| title_fullStr | The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term Freezing |
| title_full_unstemmed | The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term Freezing |
| title_short | The Morphological and Functional Properties of <i>Lactiplantibacillus plantarum</i> B411 Subjected to Acid, Bile and Heat Multi-Stress Adaptation Process and Subsequent Long-Term Freezing |
| title_sort | morphological and functional properties of i lactiplantibacillus plantarum i b411 subjected to acid bile and heat multi stress adaptation process and subsequent long term freezing |
| topic | <i>Lactiplantibacillus plantarum</i> stress preadaptation probiotics freezing |
| url | https://www.mdpi.com/2036-7481/13/4/64 |
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