NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGY
The review bears on basic principles and technologies of next-generation sequencing (NGS), as well as its applications for detection of gene mutations in leukemic cells. We discuss some novel data concerning NGS approach to studies of genetic heterogeneity in myeloproliferative disorders, detection...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
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ABV-press
2017-01-01
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| Series: | Онкогематология |
| Subjects: | |
| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/217 |
| _version_ | 1826557131813289984 |
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| author | I. M. Barkhatov A. V. Predeus A. B. Chukhlovin |
| author_facet | I. M. Barkhatov A. V. Predeus A. B. Chukhlovin |
| author_sort | I. M. Barkhatov |
| collection | DOAJ |
| description | The review bears on basic principles and technologies of next-generation sequencing (NGS), as well as its applications for detection of gene mutations in leukemic cells. We discuss some novel data concerning NGS approach to studies of genetic heterogeneity in myeloproliferative disorders, detection of high-risk genes, including drug resistance mutations, epigenomic changes associated with leukemias, as well as molecular aspects of clonal evolution. A special section concerns basic problems with bioinformatics and adequate analysis of large digital databases obtained with NGS approach. Optimal choice of appropriate software is of utmost importance for adequate retrieval and interpretation of the NGS data. |
| first_indexed | 2024-04-09T20:24:59Z |
| format | Article |
| id | doaj.art-1658933ecc8f4e5c92d3a99405149a0d |
| institution | Directory Open Access Journal |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| last_indexed | 2025-03-14T08:23:44Z |
| publishDate | 2017-01-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj.art-1658933ecc8f4e5c92d3a99405149a0d2025-03-02T13:07:10ZrusABV-pressОнкогематология1818-83462413-40232017-01-01114566310.17650/1818-8346-2016-11-4-22-32218NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGYI. M. Barkhatov0A. V. Predeus1A. B. Chukhlovin2R.M. Gorbacheva Memorial Research Institute of Children Oncology, Hematology and Transplantation, I.P. Pavlov First Saint Petersburg State Medical UniversityBioinformatics Institute, Saint Petersburg National Research Academic University of the Russian Academy of SciencesR.M. Gorbacheva Memorial Research Institute of Children Oncology, Hematology and Transplantation, I.P. Pavlov First Saint Petersburg State Medical UniversityThe review bears on basic principles and technologies of next-generation sequencing (NGS), as well as its applications for detection of gene mutations in leukemic cells. We discuss some novel data concerning NGS approach to studies of genetic heterogeneity in myeloproliferative disorders, detection of high-risk genes, including drug resistance mutations, epigenomic changes associated with leukemias, as well as molecular aspects of clonal evolution. A special section concerns basic problems with bioinformatics and adequate analysis of large digital databases obtained with NGS approach. Optimal choice of appropriate software is of utmost importance for adequate retrieval and interpretation of the NGS data.https://oncohematology.abvpress.ru/ongm/article/view/217next generation sequencingtechnologiesleukemiagene mutationsbioinformatics |
| spellingShingle | I. M. Barkhatov A. V. Predeus A. B. Chukhlovin NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGY Онкогематология next generation sequencing technologies leukemia gene mutations bioinformatics |
| title | NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGY |
| title_full | NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGY |
| title_fullStr | NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGY |
| title_full_unstemmed | NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGY |
| title_short | NEXT-GENERATION GENE SEQUENCING AND ITS APPLICATIONS IN ONCOHEMATOLOGY |
| title_sort | next generation gene sequencing and its applications in oncohematology |
| topic | next generation sequencing technologies leukemia gene mutations bioinformatics |
| url | https://oncohematology.abvpress.ru/ongm/article/view/217 |
| work_keys_str_mv | AT imbarkhatov nextgenerationgenesequencinganditsapplicationsinoncohematology AT avpredeus nextgenerationgenesequencinganditsapplicationsinoncohematology AT abchukhlovin nextgenerationgenesequencinganditsapplicationsinoncohematology |