Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.)
Abstract Background Vernalization and the transition from vegetative to reproductive growth involve multiple pathways, vital for controlling floral organ formation and flowering time. However, little transcription information is available about the mechanisms behind environmental adaption and growth...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-017-1170-8 |
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| author | Guangyan Feng Linkai Huang Ji Li Jianping Wang Lei Xu Ling Pan Xinxin Zhao Xia Wang Ting Huang Xinquan Zhang |
| author_facet | Guangyan Feng Linkai Huang Ji Li Jianping Wang Lei Xu Ling Pan Xinxin Zhao Xia Wang Ting Huang Xinquan Zhang |
| author_sort | Guangyan Feng |
| collection | DOAJ |
| description | Abstract Background Vernalization and the transition from vegetative to reproductive growth involve multiple pathways, vital for controlling floral organ formation and flowering time. However, little transcription information is available about the mechanisms behind environmental adaption and growth regulation. Here, we used high-throughput sequencing to analyze the comprehensive transcriptome of Dactylis glomerata L. during six different growth periods. Results During vernalization, 4689 differentially expressed genes (DEGs) significantly increased in abundance, while 3841 decreased. Furthermore, 12,967 DEGs were identified during booting stage and flowering stage, including 7750 up-regulated and 5219 down-regulated DEGs. Pathway analysis indicated that transcripts related to circadian rhythm, photoperiod, photosynthesis, flavonoid biosynthesis, starch, and sucrose metabolism changed significantly at different stages. Coexpression and weighted correlation network analysis (WGCNA) analysis linked different stages to transcriptional changes and provided evidence of inner relation modules associated with signal transduction, stress responses, cell division, and hormonal transport. Conclusions We found enrichment in transcription factors (TFs) related to WRKY, NAC, AP2/EREBP, AUX/IAA, MADS-BOX, ABI3/VP1, bHLH, and the CCAAT family during vernalization and floral bud development. TFs expression patterns revealed intricate temporal variations, suggesting relatively separate regulatory programs of TF modules. Further study will unlock insights into the ability of the circadian rhythm and photoperiod to regulate vernalization and flowering time in perennial grass. |
| first_indexed | 2024-12-22T13:56:07Z |
| format | Article |
| id | doaj.art-165ae07359c9433d84e49dd405d2b62f |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-22T13:56:07Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-165ae07359c9433d84e49dd405d2b62f2022-12-21T18:23:32ZengBMCBMC Plant Biology1471-22292017-11-0117111910.1186/s12870-017-1170-8Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.)Guangyan Feng0Linkai Huang1Ji Li2Jianping Wang3Lei Xu4Ling Pan5Xinxin Zhao6Xia Wang7Ting Huang8Xinquan Zhang9Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityAgronomy Department, University of FloridaDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityDepartment of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural UniversityAbstract Background Vernalization and the transition from vegetative to reproductive growth involve multiple pathways, vital for controlling floral organ formation and flowering time. However, little transcription information is available about the mechanisms behind environmental adaption and growth regulation. Here, we used high-throughput sequencing to analyze the comprehensive transcriptome of Dactylis glomerata L. during six different growth periods. Results During vernalization, 4689 differentially expressed genes (DEGs) significantly increased in abundance, while 3841 decreased. Furthermore, 12,967 DEGs were identified during booting stage and flowering stage, including 7750 up-regulated and 5219 down-regulated DEGs. Pathway analysis indicated that transcripts related to circadian rhythm, photoperiod, photosynthesis, flavonoid biosynthesis, starch, and sucrose metabolism changed significantly at different stages. Coexpression and weighted correlation network analysis (WGCNA) analysis linked different stages to transcriptional changes and provided evidence of inner relation modules associated with signal transduction, stress responses, cell division, and hormonal transport. Conclusions We found enrichment in transcription factors (TFs) related to WRKY, NAC, AP2/EREBP, AUX/IAA, MADS-BOX, ABI3/VP1, bHLH, and the CCAAT family during vernalization and floral bud development. TFs expression patterns revealed intricate temporal variations, suggesting relatively separate regulatory programs of TF modules. Further study will unlock insights into the ability of the circadian rhythm and photoperiod to regulate vernalization and flowering time in perennial grass.http://link.springer.com/article/10.1186/s12870-017-1170-8Dactylis glomerata L.Flowering regulationHigh-throughput sequencingOrchardgrassTranscriptomeVernalization |
| spellingShingle | Guangyan Feng Linkai Huang Ji Li Jianping Wang Lei Xu Ling Pan Xinxin Zhao Xia Wang Ting Huang Xinquan Zhang Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.) BMC Plant Biology Dactylis glomerata L. Flowering regulation High-throughput sequencing Orchardgrass Transcriptome Vernalization |
| title | Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.) |
| title_full | Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.) |
| title_fullStr | Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.) |
| title_full_unstemmed | Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.) |
| title_short | Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.) |
| title_sort | comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass dactylis glomerata l |
| topic | Dactylis glomerata L. Flowering regulation High-throughput sequencing Orchardgrass Transcriptome Vernalization |
| url | http://link.springer.com/article/10.1186/s12870-017-1170-8 |
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