Analysis of GPI-Anchored Receptor Distribution and Dynamics in Live Cells by Tag-Mediated Enzymatic Labeling and FRET

Analysis of GPI-Anchored Receptor Distribution and Dynamics in Live Cells by Tag-Mediated Enzymatic Labeling and FRET

The analysis of glycosylphosphatidylinositol (GPI)-anchored receptor distribution and dynamics in live cells is challenging, because their clusters exhibit subdiffraction-limited sizes and are highly dynamic. However, the cellular response depends on the GPI-anchored receptor clusters’ distribution...

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Bibliographic Details
Main Authors: Maria N. Balatskaya, Alexandra I. Baglay, Yury P. Rubtsov, George V. Sharonov
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Methods and Protocols
Subjects:
FRET
membrane proteins
GPI-anchored receptors
protein labeling
fluorescent labeling
S6 tag
Online Access:https://www.mdpi.com/2409-9279/3/2/33

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