Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture
Biodegradable polyhydroxyalkanoates, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, d...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2227-7080/11/4/106 |
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| author | Tatiana G. Volova Aleksey V. Demidenko Anastasiya V. Murueva Alexey E. Dudaev Ivan Nemtsev Ekaterina I. Shishatskaya |
| author_facet | Tatiana G. Volova Aleksey V. Demidenko Anastasiya V. Murueva Alexey E. Dudaev Ivan Nemtsev Ekaterina I. Shishatskaya |
| author_sort | Tatiana G. Volova |
| collection | DOAJ |
| description | Biodegradable polyhydroxyalkanoates, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, doripinem, and actovegin have been obtained. The loading of membranes with drugs differently affected the size of fibers and the structure of membranes, and in all cases increased the hydrophilicity of the surface. The release of drugs in vitro was gradual, which corresponded to the Higuchi and Korsmeyer-Peppas models. Antibiotic-loaded membranes showed antibacterial activity against <i>S. aureus</i> and <i>E. coli</i>, in which growth inhibition zones were 41.7 ± 1.1 and 38.6 ± 1.7 mm for ceftazidime and doripinem, respectively. The study of the biological activity of membranes in the NIH 3T3 mouse fibroblast culture based on the results of DAPI and FITC staining of cells, as well as the MTT test, did not reveal a negative effect despite the presence of antibiotics in them. Samples containing actovegin exhibit a stimulating effect on fibroblasts. Biodegradable polyhydroxyalkanoates formed by 3-hydroxybutyrate and 4-hydroxybutyrate monomers provide electrospinning non-woven membranes suitable for long-term delivery of drugs and cultivation of eukaryotic cells, and are promising for the treatment of wound defects complicated by infection. |
| first_indexed | 2024-03-10T23:32:18Z |
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| issn | 2227-7080 |
| language | English |
| last_indexed | 2024-03-10T23:32:18Z |
| publishDate | 2023-08-01 |
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| series | Technologies |
| spelling | doaj.art-165c6281bf7f4ce5a3d64ebf1a9158bd2023-11-19T03:13:30ZengMDPI AGTechnologies2227-70802023-08-0111410610.3390/technologies11040106Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell CultureTatiana G. Volova0Aleksey V. Demidenko1Anastasiya V. Murueva2Alexey E. Dudaev3Ivan Nemtsev4Ekaterina I. Shishatskaya5Institute of Biophysics SB RAS, Federal Research Center “KrasnoyarskScience Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “KrasnoyarskScience Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “KrasnoyarskScience Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “KrasnoyarskScience Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaInstitute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi av., 660041 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “KrasnoyarskScience Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaBiodegradable polyhydroxyalkanoates, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, doripinem, and actovegin have been obtained. The loading of membranes with drugs differently affected the size of fibers and the structure of membranes, and in all cases increased the hydrophilicity of the surface. The release of drugs in vitro was gradual, which corresponded to the Higuchi and Korsmeyer-Peppas models. Antibiotic-loaded membranes showed antibacterial activity against <i>S. aureus</i> and <i>E. coli</i>, in which growth inhibition zones were 41.7 ± 1.1 and 38.6 ± 1.7 mm for ceftazidime and doripinem, respectively. The study of the biological activity of membranes in the NIH 3T3 mouse fibroblast culture based on the results of DAPI and FITC staining of cells, as well as the MTT test, did not reveal a negative effect despite the presence of antibiotics in them. Samples containing actovegin exhibit a stimulating effect on fibroblasts. Biodegradable polyhydroxyalkanoates formed by 3-hydroxybutyrate and 4-hydroxybutyrate monomers provide electrospinning non-woven membranes suitable for long-term delivery of drugs and cultivation of eukaryotic cells, and are promising for the treatment of wound defects complicated by infection.https://www.mdpi.com/2227-7080/11/4/106degradable polyhydroxyalkanoatescopolymers P(3HB-co-4HB)electrostatic moldingmembranesmicrostructure and propertiesdrugs |
| spellingShingle | Tatiana G. Volova Aleksey V. Demidenko Anastasiya V. Murueva Alexey E. Dudaev Ivan Nemtsev Ekaterina I. Shishatskaya Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture Technologies degradable polyhydroxyalkanoates copolymers P(3HB-co-4HB) electrostatic molding membranes microstructure and properties drugs |
| title | Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture |
| title_full | Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture |
| title_fullStr | Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture |
| title_full_unstemmed | Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture |
| title_short | Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture |
| title_sort | biodegradable polyhydroxyalkanoates formed by 3 and 4 hydroxybutyrate monomers to produce nanomembranes suitable for drug delivery and cell culture |
| topic | degradable polyhydroxyalkanoates copolymers P(3HB-co-4HB) electrostatic molding membranes microstructure and properties drugs |
| url | https://www.mdpi.com/2227-7080/11/4/106 |
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