Bilateral Meningioma: A Case Report and Review of the Literature
Here, we present a rarely seen example of bilateral meningiomas exhibiting different malignancy grades, I (meningothelial) and II (atypical), recorded in a 72-year-old patient. The presence of two separated lesions of different grades in a single patient can elucidate meningioma progression. To this...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1187 |
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| author | Anja Bukovac Hana Panić Tomislava Mrgan Nika Šlaus Anja Kafka Niko Njirić Nives Pećina-Šlaus |
| author_facet | Anja Bukovac Hana Panić Tomislava Mrgan Nika Šlaus Anja Kafka Niko Njirić Nives Pećina-Šlaus |
| author_sort | Anja Bukovac |
| collection | DOAJ |
| description | Here, we present a rarely seen example of bilateral meningiomas exhibiting different malignancy grades, I (meningothelial) and II (atypical), recorded in a 72-year-old patient. The presence of two separated lesions of different grades in a single patient can elucidate meningioma progression. To this end, the involvement of specific protein markers of epithelial to mesenchymal transition (EMT), the process responsible for progression, was tested in both tumors. Protein expression status of specific epithelial (E-cadherin) and mesenchymal markers (N-cadherin, SNAIL&SLUG and TWIST1) was investigated. Furthermore, markers that are connected to Wnt signaling pathway–beta-catenin, GSK3beta and DVL1—were also analyzed. For signs of neurofibromatosis and schwanomatosis genetic testing was performed. Immunohistochemistry evaluated by immunoreactivity score (IRS) was used to determine the signal strengths and proteins’ location. Our results indicated that, in comparison to the grade I tumor, mesenchymal markers SNAIL and SLUG were upregulated in the atypical meningioma. TWIST1, beta-catenin and GSK3beta were upregulated in both grades, while E-cadherin was partially lost. A pronounced cadherin switch could not be established; however, N-cadherin showed widespread tissue presence. Genetic testing did not detect changes of <i>NF2</i> or <i>SMARCB1</i> genes denying germline origin of the lesions. The rare presence of two different grades in one patient elucidate previously unknown molecules involved in meningioma progression. |
| first_indexed | 2024-03-09T23:49:23Z |
| format | Article |
| id | doaj.art-166230a7df4740b88a1f5cbbaf34c741 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:49:23Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-166230a7df4740b88a1f5cbbaf34c7412023-11-23T16:36:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233118710.3390/ijms23031187Bilateral Meningioma: A Case Report and Review of the LiteratureAnja Bukovac0Hana Panić1Tomislava Mrgan2Nika Šlaus3Anja Kafka4Niko Njirić5Nives Pećina-Šlaus6Laboratory of Neuro-Oncology, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Šalata 12, 10000 Zagreb, CroatiaSchool of Medicine, University of Zagreb, Šalata 3, 10000 Zagreb, CroatiaSchool of Medicine, University of Zagreb, Šalata 3, 10000 Zagreb, CroatiaSchool of Medicine, University of Zagreb, Šalata 3, 10000 Zagreb, CroatiaLaboratory of Neuro-Oncology, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Šalata 12, 10000 Zagreb, CroatiaDepartment of Neurosurgery, University Hospital Center “Zagreb”, School of Medicine, University of Zagreb, Kišpatićeva 12, 10000 Zagreb, CroatiaLaboratory of Neuro-Oncology, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Šalata 12, 10000 Zagreb, CroatiaHere, we present a rarely seen example of bilateral meningiomas exhibiting different malignancy grades, I (meningothelial) and II (atypical), recorded in a 72-year-old patient. The presence of two separated lesions of different grades in a single patient can elucidate meningioma progression. To this end, the involvement of specific protein markers of epithelial to mesenchymal transition (EMT), the process responsible for progression, was tested in both tumors. Protein expression status of specific epithelial (E-cadherin) and mesenchymal markers (N-cadherin, SNAIL&SLUG and TWIST1) was investigated. Furthermore, markers that are connected to Wnt signaling pathway–beta-catenin, GSK3beta and DVL1—were also analyzed. For signs of neurofibromatosis and schwanomatosis genetic testing was performed. Immunohistochemistry evaluated by immunoreactivity score (IRS) was used to determine the signal strengths and proteins’ location. Our results indicated that, in comparison to the grade I tumor, mesenchymal markers SNAIL and SLUG were upregulated in the atypical meningioma. TWIST1, beta-catenin and GSK3beta were upregulated in both grades, while E-cadherin was partially lost. A pronounced cadherin switch could not be established; however, N-cadherin showed widespread tissue presence. Genetic testing did not detect changes of <i>NF2</i> or <i>SMARCB1</i> genes denying germline origin of the lesions. The rare presence of two different grades in one patient elucidate previously unknown molecules involved in meningioma progression.https://www.mdpi.com/1422-0067/23/3/1187multiple meningiomabilateral meningiomaEMTWnt signalingE-cadherinN-cadherin |
| spellingShingle | Anja Bukovac Hana Panić Tomislava Mrgan Nika Šlaus Anja Kafka Niko Njirić Nives Pećina-Šlaus Bilateral Meningioma: A Case Report and Review of the Literature International Journal of Molecular Sciences multiple meningioma bilateral meningioma EMT Wnt signaling E-cadherin N-cadherin |
| title | Bilateral Meningioma: A Case Report and Review of the Literature |
| title_full | Bilateral Meningioma: A Case Report and Review of the Literature |
| title_fullStr | Bilateral Meningioma: A Case Report and Review of the Literature |
| title_full_unstemmed | Bilateral Meningioma: A Case Report and Review of the Literature |
| title_short | Bilateral Meningioma: A Case Report and Review of the Literature |
| title_sort | bilateral meningioma a case report and review of the literature |
| topic | multiple meningioma bilateral meningioma EMT Wnt signaling E-cadherin N-cadherin |
| url | https://www.mdpi.com/1422-0067/23/3/1187 |
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