Salvianolic acid B: In vitro and in vivo effects on the immune system
Type 1 diabetes (T1D) is an autoimmune disorder with a strong inflammatory component. Autoreactive cells specifically target insulin-producing β-cells, which leads to loss of glucose homeostasis. T1D remains incurable and versatile; potentially beneficial therapeutics are being tested world...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641700011V.pdf |
| Summary: | Type 1 diabetes (T1D) is an autoimmune disorder with a strong inflammatory
component. Autoreactive cells specifically target insulin-producing β-cells,
which leads to loss of glucose homeostasis. T1D remains incurable and
versatile; potentially beneficial therapeutics are being tested worldwide.
Possible candidates for the treatment of autoimmune diabetes are plants and
their extracts since they are rich in biophenols, substances that act as
secondary metabolites, and have verified antioxidant and antiinflammatory
effects. Salvianolic acid B (SalB) is a biophenol and one of the major
constituents of Origanum vulgare ssp. hirtum (Greek oregano) extracts which
in our previous studies was shown to exhibit an antidiabetic effect in mice.
The aim of the present study was to determine whether SalB is responsible for
the observed effects of Greek oregano extracts. SalB was applied in vitro to
macrophages and lymphocytes isolated from C57BL/6 mice, as well as in vivo in
the model of T1D induced by multiple low doses (MLD) of streptozotocin (STZ).
SalB did not affect the viability of cells, but it significantly decreased
secretion of nitric oxide (NO) and TNF in lipopolysaccharide (LPS)-stimulated
macrophages, as well as the secretion of IFN-γ in concanavalin A
(ConA)-stimulated lymphocytes. However, when applied in vivo, SalB at a dose
of 2.5 mg/kg b.w., applied for 10 consecutive days, failed to protect mice
from diabetes development. In conclusion, SalB exerts immunomodulatory
effects in vitro, but is not effective in prevention of T1D in vivo. It
probably requires cooperation with some other substances for the maximum
efficacy exhibited by oregano extracts. [Project of the Serbian Ministry of
Education, Science and Technological Development, Grant no. OI 173013] |
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| ISSN: | 0354-4664 1821-4339 |