Vitamin B12 and ketorolac on pain in Long Evans rats
Background: Effects of vitamin B12 on pain have been demonstrated in different animal and humanstudies. But comparison of these effects with similar effects of ketorolac tromethamine (KT) and theircombination have not been established. Objective: To assess the effects of vitamin B12 on pain andalso...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Bangladesh Society of Physiologist (BSP)
2016-12-01
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| Series: | Journal of Bangladesh Society of Physiologists |
| Subjects: | |
| Online Access: | http://www.banglajol.info/index.php/JBSP/article/view/30653 |
| _version_ | 1818566688741785600 |
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| author | Mizanur Rahman Noorzahan Begum Taskina Ali Mahadi Abdur Rouf Shahriar Masood |
| author_facet | Mizanur Rahman Noorzahan Begum Taskina Ali Mahadi Abdur Rouf Shahriar Masood |
| author_sort | Mizanur Rahman |
| collection | DOAJ |
| description | Background: Effects of vitamin B12 on pain have been demonstrated in different animal and humanstudies. But comparison of these effects with similar effects of ketorolac tromethamine (KT) and theircombination have not been established. Objective: To assess the effects of vitamin B12 on pain andalso to compare them with those of the combinations of vitamin B12 with KT in rat models. Methods:This experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh MujibMedical University (BSMMU), Dhaka, from March 2015 to February 2016. For this, 20 (twenty)Long Evans rats (215±35 gm) of both sexes were divided into control (A, with 5 ml/kg normal saline)and experimental (B1, with 15 mg/kg B12; B2, with 10 mg/kg KT; B3, with B12+KT) groups with 5rats in each group. All the drugs and vitamin were administered intraperitoneally in a single dose justone hour before formalin test. To evaluate the treatments’ effect on nociceptive pain, early phase (1st-5th minutes); on central analgesic system, interphase (6th-15th minutes); and on inflammatory pain,late phase (16th-60th minutes) of the formalin test, were observed. In all phases, total frequency ofjerking and total duration of flexing and licking of the right hind paw were counted after administrationof subcutaneous formalin (50 μl, 2.5%) injection. Statistical analysis was done by ANOVA, followedby Bonferroni post hoc test. In the interpretation of results, p≤0.05 was considered as significant.Results: B12 lowered only the jerking frequency and KT lowered both jerking frequency and flexinglicking
duration significantly (p≤0.001) in the late phase of formalin test. On the other hand, combinationof B12 and KT significantly (p≤0.001) lowered both the study variables in all 3 phases of formalintest. Conclusion: From this study it may be concluded that, vitamin B12 possess analgesic effects andcombination of B12 with KT is more effective than those of their individual administration. |
| first_indexed | 2024-12-14T01:56:48Z |
| format | Article |
| id | doaj.art-1665bab8b6394c4697d77f8bab5541f9 |
| institution | Directory Open Access Journal |
| issn | 1995-1213 2219-7508 |
| language | English |
| last_indexed | 2024-12-14T01:56:48Z |
| publishDate | 2016-12-01 |
| publisher | Bangladesh Society of Physiologist (BSP) |
| record_format | Article |
| series | Journal of Bangladesh Society of Physiologists |
| spelling | doaj.art-1665bab8b6394c4697d77f8bab5541f92022-12-21T23:21:10ZengBangladesh Society of Physiologist (BSP)Journal of Bangladesh Society of Physiologists1995-12132219-75082016-12-011126369http://dx.doi.org/10.3329/jbsp.v11i2.30653Vitamin B12 and ketorolac on pain in Long Evans ratsMizanur Rahman0Noorzahan Begum1Taskina Ali2Mahadi Abdur Rouf3Shahriar Masood4Department of Physiology, Enam Medical College, BangladeshDepartment of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), BangladeshDepartment of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), BangladeshDepartment of Physiology, Northern International Medical College, BangladeshDepartment of Physiology, Jahurul Islam Medical College, Bhagalpur, Bajitpur, Kishoregonj BangladeshBackground: Effects of vitamin B12 on pain have been demonstrated in different animal and humanstudies. But comparison of these effects with similar effects of ketorolac tromethamine (KT) and theircombination have not been established. Objective: To assess the effects of vitamin B12 on pain andalso to compare them with those of the combinations of vitamin B12 with KT in rat models. Methods:This experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh MujibMedical University (BSMMU), Dhaka, from March 2015 to February 2016. For this, 20 (twenty)Long Evans rats (215±35 gm) of both sexes were divided into control (A, with 5 ml/kg normal saline)and experimental (B1, with 15 mg/kg B12; B2, with 10 mg/kg KT; B3, with B12+KT) groups with 5rats in each group. All the drugs and vitamin were administered intraperitoneally in a single dose justone hour before formalin test. To evaluate the treatments’ effect on nociceptive pain, early phase (1st-5th minutes); on central analgesic system, interphase (6th-15th minutes); and on inflammatory pain,late phase (16th-60th minutes) of the formalin test, were observed. In all phases, total frequency ofjerking and total duration of flexing and licking of the right hind paw were counted after administrationof subcutaneous formalin (50 μl, 2.5%) injection. Statistical analysis was done by ANOVA, followedby Bonferroni post hoc test. In the interpretation of results, p≤0.05 was considered as significant.Results: B12 lowered only the jerking frequency and KT lowered both jerking frequency and flexinglicking duration significantly (p≤0.001) in the late phase of formalin test. On the other hand, combinationof B12 and KT significantly (p≤0.001) lowered both the study variables in all 3 phases of formalintest. Conclusion: From this study it may be concluded that, vitamin B12 possess analgesic effects andcombination of B12 with KT is more effective than those of their individual administration.http://www.banglajol.info/index.php/JBSP/article/view/30653PainAnalgesicVitamin B12Ketorolac |
| spellingShingle | Mizanur Rahman Noorzahan Begum Taskina Ali Mahadi Abdur Rouf Shahriar Masood Vitamin B12 and ketorolac on pain in Long Evans rats Journal of Bangladesh Society of Physiologists Pain Analgesic Vitamin B12 Ketorolac |
| title | Vitamin B12 and ketorolac on pain in Long Evans rats |
| title_full | Vitamin B12 and ketorolac on pain in Long Evans rats |
| title_fullStr | Vitamin B12 and ketorolac on pain in Long Evans rats |
| title_full_unstemmed | Vitamin B12 and ketorolac on pain in Long Evans rats |
| title_short | Vitamin B12 and ketorolac on pain in Long Evans rats |
| title_sort | vitamin b12 and ketorolac on pain in long evans rats |
| topic | Pain Analgesic Vitamin B12 Ketorolac |
| url | http://www.banglajol.info/index.php/JBSP/article/view/30653 |
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