Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts
Abstract Background Platelet aggregation and advanced glycation end products (AGEs) and oxidative stress are known as key factors for the development of cardiovascular diseases and diabetic complications. In this context, fruit and vegetable consumption, good sources of antioxidant compounds have be...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-022-03824-6 |
| _version_ | 1797973757795500032 |
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| author | Zahra Amri Ikram Ben Amor Amira Zarrouk Raja Chaaba Jalel Gargouri Mohamed Hammami Sonia Hammami |
| author_facet | Zahra Amri Ikram Ben Amor Amira Zarrouk Raja Chaaba Jalel Gargouri Mohamed Hammami Sonia Hammami |
| author_sort | Zahra Amri |
| collection | DOAJ |
| description | Abstract Background Platelet aggregation and advanced glycation end products (AGEs) and oxidative stress are known as key factors for the development of cardiovascular diseases and diabetic complications. In this context, fruit and vegetable consumption, good sources of antioxidant compounds have been largely reported as an effective way of preventing human against these diseases. The current study focuses on the evaluation of antioxidant, antiplatelet and anti-glycation activities of pomegranate (Punica granatum L.) flowers (PF), leaves (PL), peel (PP) juice (PJ) and seeds oil (PSO). Methods Antioxidant activities was measured against ABTS radical and lipid peroxidation. Antiglycation activity was determined using the formation of AGE fluorescence intensity in the BSA/ribose system. Antiplatelet activity was measured in platelet rich plasma (PRP) against adenosine diphosphate (ADP), Collagen and arachidonic acid (AA). Results PF extract displayed the highest antioxidant activity against ABTS and lipid peroxidation with IC50 values of 0.7 mg/mL and 0.63 mg/mL respectively. For anti-glycation activity, PP, PF and PL inhibited moderately the pentosidine-like AGEs formation compared to positive controls with AGE-IC50 value of 0.4 mg/mL. PJ and PSO haven’t any anti-AGE effect. All the extracts selectively inhibited platelet aggregation caused by one, two or three inducers in dose dependent manner. PF was the most potent inhibitor caused by all three inducers, with inhibitory effects ranging from 35.6 to 66.6%. PP and PJ exhibited antiplatelet effect against both ADP and collagen and PL and PSO only against AA. Conclusions These results suggest that some pomegranate extracts exert potential in vitro anti-glycative and antiplatelet activities. |
| first_indexed | 2024-04-11T04:08:10Z |
| format | Article |
| id | doaj.art-166839b99d214dcab310a813eda77cb5 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-04-11T04:08:10Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-166839b99d214dcab310a813eda77cb52023-01-01T12:12:56ZengBMCBMC Complementary Medicine and Therapies2662-76712022-12-012211710.1186/s12906-022-03824-6Anti-glycation, antiplatelet and antioxidant effects of different pomegranate partsZahra Amri0Ikram Ben Amor1Amira Zarrouk2Raja Chaaba3Jalel Gargouri4Mohamed Hammami5Sonia Hammami6Biochemistry Laboratory, LR12ES05 “Nutrition- Functional Foods and vascular Health”, Faculty of Medicine, University of MonastirCentre Régional de Transfusion Sanguine de SfaxBiochemistry Laboratory, LR12ES05 “Nutrition- Functional Foods and vascular Health”, Faculty of Medicine, University of MonastirBiochemistry Laboratory, LR12ES05 “Nutrition- Functional Foods and vascular Health”, Faculty of Medicine, University of MonastirCentre Régional de Transfusion Sanguine de SfaxBiochemistry Laboratory, LR12ES05 “Nutrition- Functional Foods and vascular Health”, Faculty of Medicine, University of MonastirBiochemistry Laboratory, LR12ES05 “Nutrition- Functional Foods and vascular Health”, Faculty of Medicine, University of MonastirAbstract Background Platelet aggregation and advanced glycation end products (AGEs) and oxidative stress are known as key factors for the development of cardiovascular diseases and diabetic complications. In this context, fruit and vegetable consumption, good sources of antioxidant compounds have been largely reported as an effective way of preventing human against these diseases. The current study focuses on the evaluation of antioxidant, antiplatelet and anti-glycation activities of pomegranate (Punica granatum L.) flowers (PF), leaves (PL), peel (PP) juice (PJ) and seeds oil (PSO). Methods Antioxidant activities was measured against ABTS radical and lipid peroxidation. Antiglycation activity was determined using the formation of AGE fluorescence intensity in the BSA/ribose system. Antiplatelet activity was measured in platelet rich plasma (PRP) against adenosine diphosphate (ADP), Collagen and arachidonic acid (AA). Results PF extract displayed the highest antioxidant activity against ABTS and lipid peroxidation with IC50 values of 0.7 mg/mL and 0.63 mg/mL respectively. For anti-glycation activity, PP, PF and PL inhibited moderately the pentosidine-like AGEs formation compared to positive controls with AGE-IC50 value of 0.4 mg/mL. PJ and PSO haven’t any anti-AGE effect. All the extracts selectively inhibited platelet aggregation caused by one, two or three inducers in dose dependent manner. PF was the most potent inhibitor caused by all three inducers, with inhibitory effects ranging from 35.6 to 66.6%. PP and PJ exhibited antiplatelet effect against both ADP and collagen and PL and PSO only against AA. Conclusions These results suggest that some pomegranate extracts exert potential in vitro anti-glycative and antiplatelet activities.https://doi.org/10.1186/s12906-022-03824-6PomegranateAntiplatelet activityAdvanced glycation end-productsOxidative stressLipid peroxidation |
| spellingShingle | Zahra Amri Ikram Ben Amor Amira Zarrouk Raja Chaaba Jalel Gargouri Mohamed Hammami Sonia Hammami Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts BMC Complementary Medicine and Therapies Pomegranate Antiplatelet activity Advanced glycation end-products Oxidative stress Lipid peroxidation |
| title | Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts |
| title_full | Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts |
| title_fullStr | Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts |
| title_full_unstemmed | Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts |
| title_short | Anti-glycation, antiplatelet and antioxidant effects of different pomegranate parts |
| title_sort | anti glycation antiplatelet and antioxidant effects of different pomegranate parts |
| topic | Pomegranate Antiplatelet activity Advanced glycation end-products Oxidative stress Lipid peroxidation |
| url | https://doi.org/10.1186/s12906-022-03824-6 |
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