Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol
The use of tail chalk and estrus/heat expression scores (HEATSC) evaluation is instrumental in identifying cows with greater estrus expression and greater artificial insemination pregnancy rates (P/AI) in cows submitted to timed artificial insemination (TAI), and cows with low or no estrus expressio...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731119000454 |
| _version_ | 1819015315503185920 |
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| author | W.B. Rodrigues A.S. Silva J.C.B. Silva N.A. Anache K.C. Silva C.J.T. Cardoso W.R. Garcia P. Sutovsky E. Nogueira |
| author_facet | W.B. Rodrigues A.S. Silva J.C.B. Silva N.A. Anache K.C. Silva C.J.T. Cardoso W.R. Garcia P. Sutovsky E. Nogueira |
| author_sort | W.B. Rodrigues |
| collection | DOAJ |
| description | The use of tail chalk and estrus/heat expression scores (HEATSC) evaluation is instrumental in identifying cows with greater estrus expression and greater artificial insemination pregnancy rates (P/AI) in cows submitted to timed artificial insemination (TAI), and cows with low or no estrus expression present lower P/AI. It was intended in this study to improve the pregnancy rates in TAI for Bos indicus beef cows, and gonadotrophin-releasing hormone (GnRH) injection was hypothesized to increase pregnancy rates in a TAI program for cows submitted to progesterone–estradiol-based protocols with low or no estrus expression, evaluated by HEATSC. Cows (n= 2284) received a progesterone device and 2 mg estradiol benzoate, after 8 days the device was removed and 1 mg estradiol cypionate, 150 μg of d-cloprostenol and 300 IU equine chorionic gonadotropin was administered. All cows were marked with chalk and HEATSC evaluated (scales 1 to 3) at TAI performed on day 10. Animals with HEATSC1 and HEATSC2 (n= 937) received 100 μg de gonadorelin (GNRH group; n= 470), or 1 ml saline (Control group; n= 467), and cows with HEATSC3 (named HEAT group; n= 1347) received no additional treatment. The larger dominant follicle, evaluated on day 8and at TAI (day 10), was greater in HEAT group (P= 0.0145 and P <0.001, respectively). Corpus luteum (CL) area and progesterone concentration was evaluated on day 17, and CL area was larger in HEAT group, intermediary in Control and lower in GnRH group (Control= 2.68 cm2, GnRH= 2.37 cm2, HEAT group= 3.07 cm2, P <0.001). Greater progesterone concentrations were found in HEAT group than in Control and GnRH groups (Control= 4.74 ng/ml, GnRH= 4.29 ng/ml, HEAT group= 6.08 ng/ml, P<0.001). There was a difference in ovulation rate, greater in HEAT group than GnRH and Control groups (Control= 72.5%; GnRH= 81.25%; HEAT group= 90.71%; P= 0.0024). Artificial insemination pregnancy rates was greater in HEAT group (57.09% (769/1347) than in Control and GNRH groups, with positive effect of GnRH injection at the time of TAI in P/AI (Control= 36.18% (169/467), GnRH= 45.95% (216/470); P<0.0001). In conclusion, GnRH application in cows with low HEATSC (1 and 2) is a simple strategy, requiring no changes in TAI management to increase pregnancy rates in postpartum beef cows submitted to progesterone–estradiol-based TAI protocols, without reaching, however, the pregnancy rates of cows that demonstrate high estrus expression at the TAI. |
| first_indexed | 2024-12-21T02:29:47Z |
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| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-21T02:29:47Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
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| series | Animal |
| spelling | doaj.art-1669a96e4e3f43c08209438e667240db2022-12-21T19:18:57ZengElsevierAnimal1751-73112019-01-01131023132318Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocolW.B. Rodrigues0A.S. Silva1J.C.B. Silva2N.A. Anache3K.C. Silva4C.J.T. Cardoso5W.R. Garcia6P. Sutovsky7E. Nogueira8Embrapa Pantanal, Rua 21 de Setembro, 1880, Corumbá, MS 79320-900, BrasilGenesis Tecnologia e Reprodução Animal, Rua Goncalves Dias, 390, Campo Grande, MS, 79004-210, BrasilEmbrapa Pantanal, Rua 21 de Setembro, 1880, Corumbá, MS 79320-900, BrasilPrograma de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443,Campo Grande -MS, 79074-460, BrasilPrograma de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443,Campo Grande -MS, 79074-460, BrasilPrograma de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443,Campo Grande -MS, 79074-460, BrasilPrograma de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443,Campo Grande -MS, 79074-460, BrasilDepartment of Obstetrics, Gynecology & Women’s Health, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USAEmbrapa Pantanal, Rua 21 de Setembro, 1880, Corumbá, MS 79320-900, Brasil; Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443,Campo Grande -MS, 79074-460, BrasilThe use of tail chalk and estrus/heat expression scores (HEATSC) evaluation is instrumental in identifying cows with greater estrus expression and greater artificial insemination pregnancy rates (P/AI) in cows submitted to timed artificial insemination (TAI), and cows with low or no estrus expression present lower P/AI. It was intended in this study to improve the pregnancy rates in TAI for Bos indicus beef cows, and gonadotrophin-releasing hormone (GnRH) injection was hypothesized to increase pregnancy rates in a TAI program for cows submitted to progesterone–estradiol-based protocols with low or no estrus expression, evaluated by HEATSC. Cows (n= 2284) received a progesterone device and 2 mg estradiol benzoate, after 8 days the device was removed and 1 mg estradiol cypionate, 150 μg of d-cloprostenol and 300 IU equine chorionic gonadotropin was administered. All cows were marked with chalk and HEATSC evaluated (scales 1 to 3) at TAI performed on day 10. Animals with HEATSC1 and HEATSC2 (n= 937) received 100 μg de gonadorelin (GNRH group; n= 470), or 1 ml saline (Control group; n= 467), and cows with HEATSC3 (named HEAT group; n= 1347) received no additional treatment. The larger dominant follicle, evaluated on day 8and at TAI (day 10), was greater in HEAT group (P= 0.0145 and P <0.001, respectively). Corpus luteum (CL) area and progesterone concentration was evaluated on day 17, and CL area was larger in HEAT group, intermediary in Control and lower in GnRH group (Control= 2.68 cm2, GnRH= 2.37 cm2, HEAT group= 3.07 cm2, P <0.001). Greater progesterone concentrations were found in HEAT group than in Control and GnRH groups (Control= 4.74 ng/ml, GnRH= 4.29 ng/ml, HEAT group= 6.08 ng/ml, P<0.001). There was a difference in ovulation rate, greater in HEAT group than GnRH and Control groups (Control= 72.5%; GnRH= 81.25%; HEAT group= 90.71%; P= 0.0024). Artificial insemination pregnancy rates was greater in HEAT group (57.09% (769/1347) than in Control and GNRH groups, with positive effect of GnRH injection at the time of TAI in P/AI (Control= 36.18% (169/467), GnRH= 45.95% (216/470); P<0.0001). In conclusion, GnRH application in cows with low HEATSC (1 and 2) is a simple strategy, requiring no changes in TAI management to increase pregnancy rates in postpartum beef cows submitted to progesterone–estradiol-based TAI protocols, without reaching, however, the pregnancy rates of cows that demonstrate high estrus expression at the TAI.http://www.sciencedirect.com/science/article/pii/S1751731119000454artificial inseminationfertilityfolliclereproductionmanagement |
| spellingShingle | W.B. Rodrigues A.S. Silva J.C.B. Silva N.A. Anache K.C. Silva C.J.T. Cardoso W.R. Garcia P. Sutovsky E. Nogueira Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol Animal artificial insemination fertility follicle reproduction management |
| title | Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol |
| title_full | Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol |
| title_fullStr | Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol |
| title_full_unstemmed | Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol |
| title_short | Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol |
| title_sort | timed artificial insemination plus heat ii gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone estradiol based protocol |
| topic | artificial insemination fertility follicle reproduction management |
| url | http://www.sciencedirect.com/science/article/pii/S1751731119000454 |
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