Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria
Abstract Background ICEs are mobile genetic elements found integrated into bacterial chromosomes that can excise and be transferred to a new cell. They play an important role in horizontal gene transmission and carry accessory genes that may provide interesting phenotypes for the bacteria. Here, we...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Mobile DNA |
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| Online Access: | https://doi.org/10.1186/s13100-022-00275-1 |
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| author | Jéssica Catarine Silva de Assis Osiel Silva Gonçalves Alexia Suellen Fernandes Marisa Vieira de Queiroz Denise Mara Soares Bazzolli Mateus Ferreira Santana |
| author_facet | Jéssica Catarine Silva de Assis Osiel Silva Gonçalves Alexia Suellen Fernandes Marisa Vieira de Queiroz Denise Mara Soares Bazzolli Mateus Ferreira Santana |
| author_sort | Jéssica Catarine Silva de Assis |
| collection | DOAJ |
| description | Abstract Background ICEs are mobile genetic elements found integrated into bacterial chromosomes that can excise and be transferred to a new cell. They play an important role in horizontal gene transmission and carry accessory genes that may provide interesting phenotypes for the bacteria. Here, we seek to research the presence and the role of ICEs in 300 genomes of phytopathogenic bacteria with the greatest scientific and economic impact. Results Seventy-eight ICEs (45 distinct elements) were identified and characterized in chromosomes of Agrobacterium tumefaciens , Dickeya dadantii, and D. solani, Pectobacterium carotovorum and P. atrosepticum, Pseudomonas syringae, Ralstonia solanacearum Species Complex, and Xanthomonas campestris. Intriguingly, the co-occurrence of four ICEs was observed in some P. syringae strains. Moreover, we identified 31 novel elements, carrying 396 accessory genes with potential influence on virulence and fitness, such as genes coding for functions related to T3SS, cell wall degradation and resistance to heavy metals. We also present the analysis of previously reported data on the expression of cargo genes related to the virulence of P. atrosepticum ICEs, which evidences the role of these genes in the infection process of tobacco plants. Conclusions Altogether, this paper has highlighted the potential of ICEs to affect the pathogenicity and lifestyle of these phytopathogens and direct the spread of significant putative virulence genes in phytopathogenic bacteria. |
| first_indexed | 2024-12-10T19:56:12Z |
| format | Article |
| id | doaj.art-166b41b8aee046399553ff4444379ffd |
| institution | Directory Open Access Journal |
| issn | 1759-8753 |
| language | English |
| last_indexed | 2024-12-10T19:56:12Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Mobile DNA |
| spelling | doaj.art-166b41b8aee046399553ff4444379ffd2022-12-22T01:35:39ZengBMCMobile DNA1759-87532022-08-0113111210.1186/s13100-022-00275-1Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteriaJéssica Catarine Silva de Assis0Osiel Silva Gonçalves1Alexia Suellen Fernandes2Marisa Vieira de Queiroz3Denise Mara Soares Bazzolli4Mateus Ferreira Santana5Grupo de Genômica Evolutiva Microbiana, Laboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada À Agropecuária, Universidade Federal de ViçosaGrupo de Genômica Evolutiva Microbiana, Laboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada À Agropecuária, Universidade Federal de ViçosaGrupo de Genômica Evolutiva Microbiana, Laboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada À Agropecuária, Universidade Federal de ViçosaLaboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada À Agropecuária, Universidade Federal de ViçosaLaboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada À Agropecuária, Universidade Federal de ViçosaGrupo de Genômica Evolutiva Microbiana, Laboratório de Genética Molecular de Microrganismos, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada À Agropecuária, Universidade Federal de ViçosaAbstract Background ICEs are mobile genetic elements found integrated into bacterial chromosomes that can excise and be transferred to a new cell. They play an important role in horizontal gene transmission and carry accessory genes that may provide interesting phenotypes for the bacteria. Here, we seek to research the presence and the role of ICEs in 300 genomes of phytopathogenic bacteria with the greatest scientific and economic impact. Results Seventy-eight ICEs (45 distinct elements) were identified and characterized in chromosomes of Agrobacterium tumefaciens , Dickeya dadantii, and D. solani, Pectobacterium carotovorum and P. atrosepticum, Pseudomonas syringae, Ralstonia solanacearum Species Complex, and Xanthomonas campestris. Intriguingly, the co-occurrence of four ICEs was observed in some P. syringae strains. Moreover, we identified 31 novel elements, carrying 396 accessory genes with potential influence on virulence and fitness, such as genes coding for functions related to T3SS, cell wall degradation and resistance to heavy metals. We also present the analysis of previously reported data on the expression of cargo genes related to the virulence of P. atrosepticum ICEs, which evidences the role of these genes in the infection process of tobacco plants. Conclusions Altogether, this paper has highlighted the potential of ICEs to affect the pathogenicity and lifestyle of these phytopathogens and direct the spread of significant putative virulence genes in phytopathogenic bacteria.https://doi.org/10.1186/s13100-022-00275-1Genome evolutionHorizontal gene transferMobile genetic elements (MGE)Phytopathology |
| spellingShingle | Jéssica Catarine Silva de Assis Osiel Silva Gonçalves Alexia Suellen Fernandes Marisa Vieira de Queiroz Denise Mara Soares Bazzolli Mateus Ferreira Santana Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria Mobile DNA Genome evolution Horizontal gene transfer Mobile genetic elements (MGE) Phytopathology |
| title | Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria |
| title_full | Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria |
| title_fullStr | Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria |
| title_full_unstemmed | Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria |
| title_short | Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria |
| title_sort | genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria |
| topic | Genome evolution Horizontal gene transfer Mobile genetic elements (MGE) Phytopathology |
| url | https://doi.org/10.1186/s13100-022-00275-1 |
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