Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress Conditions
This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hyd...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Soil Systems |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2571-8789/5/2/26 |
| _version_ | 1797538104931778560 |
|---|---|
| author | Rihab Djebaili Marika Pellegrini Massimiliano Rossi Cinzia Forni Maria Smati Maddalena Del Gallo Mahmoud Kitouni |
| author_facet | Rihab Djebaili Marika Pellegrini Massimiliano Rossi Cinzia Forni Maria Smati Maddalena Del Gallo Mahmoud Kitouni |
| author_sort | Rihab Djebaili |
| collection | DOAJ |
| description | This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants. |
| first_indexed | 2024-03-10T12:25:46Z |
| format | Article |
| id | doaj.art-166d21221c04404e8abb7afc5f0af813 |
| institution | Directory Open Access Journal |
| issn | 2571-8789 |
| language | English |
| last_indexed | 2024-03-10T12:25:46Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Soil Systems |
| spelling | doaj.art-166d21221c04404e8abb7afc5f0af8132023-11-21T15:00:34ZengMDPI AGSoil Systems2571-87892021-04-01522610.3390/soilsystems5020026Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress ConditionsRihab Djebaili0Marika Pellegrini1Massimiliano Rossi2Cinzia Forni3Maria Smati4Maddalena Del Gallo5Mahmoud Kitouni6Laboratory of Microbiological Engineering and Applications, Chaâbat Erssas Campus, University of Brothers Mentouri Constantine 1, Ain El Bey Road, 25000 Constantine, AlgeriaDepartment of Life, Health and Environmental Sciences, University of L’Aquila, Coppito, 67100 L’Aquila, ItalyDepartment of Biology, University of Rome “TorVergata”, 00133 Roma, ItalyDepartment of Biology, University of Rome “TorVergata”, 00133 Roma, ItalyLaboratory of Microbiological Engineering and Applications, Chaâbat Erssas Campus, University of Brothers Mentouri Constantine 1, Ain El Bey Road, 25000 Constantine, AlgeriaDepartment of Life, Health and Environmental Sciences, University of L’Aquila, Coppito, 67100 L’Aquila, ItalyLaboratory of Microbiological Engineering and Applications, Chaâbat Erssas Campus, University of Brothers Mentouri Constantine 1, Ain El Bey Road, 25000 Constantine, AlgeriaThis study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.https://www.mdpi.com/2571-8789/5/2/26PGPBactinomycetes1-aminocyclopropane-1-carboxylate deaminasedurum wheatsalt stresshalotolerance |
| spellingShingle | Rihab Djebaili Marika Pellegrini Massimiliano Rossi Cinzia Forni Maria Smati Maddalena Del Gallo Mahmoud Kitouni Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress Conditions Soil Systems PGPB actinomycetes 1-aminocyclopropane-1-carboxylate deaminase durum wheat salt stress halotolerance |
| title | Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress Conditions |
| title_full | Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress Conditions |
| title_fullStr | Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress Conditions |
| title_full_unstemmed | Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress Conditions |
| title_short | Characterization of Plant Growth-Promoting Traits and Inoculation Effects on <i>Triticum durum</i> of Actinomycetes Isolates under Salt Stress Conditions |
| title_sort | characterization of plant growth promoting traits and inoculation effects on i triticum durum i of actinomycetes isolates under salt stress conditions |
| topic | PGPB actinomycetes 1-aminocyclopropane-1-carboxylate deaminase durum wheat salt stress halotolerance |
| url | https://www.mdpi.com/2571-8789/5/2/26 |
| work_keys_str_mv | AT rihabdjebaili characterizationofplantgrowthpromotingtraitsandinoculationeffectsonitriticumdurumiofactinomycetesisolatesundersaltstressconditions AT marikapellegrini characterizationofplantgrowthpromotingtraitsandinoculationeffectsonitriticumdurumiofactinomycetesisolatesundersaltstressconditions AT massimilianorossi characterizationofplantgrowthpromotingtraitsandinoculationeffectsonitriticumdurumiofactinomycetesisolatesundersaltstressconditions AT cinziaforni characterizationofplantgrowthpromotingtraitsandinoculationeffectsonitriticumdurumiofactinomycetesisolatesundersaltstressconditions AT mariasmati characterizationofplantgrowthpromotingtraitsandinoculationeffectsonitriticumdurumiofactinomycetesisolatesundersaltstressconditions AT maddalenadelgallo characterizationofplantgrowthpromotingtraitsandinoculationeffectsonitriticumdurumiofactinomycetesisolatesundersaltstressconditions AT mahmoudkitouni characterizationofplantgrowthpromotingtraitsandinoculationeffectsonitriticumdurumiofactinomycetesisolatesundersaltstressconditions |