In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth
Statement of Problem: Indiscriminate use of antibacterial agents increases the antibiotic resistance, which consequently necessitates seeking alternatives such as herbal remedies. Objectives: The aim of this study was to evaluate the antimicrobial effect of hydroalcoholic extract of Helichrysum leu...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2017-12-01
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| Series: | Journal of Dental Biomaterial |
| Subjects: | |
| Online Access: | http://jdb.sums.ac.ir/index.php/jdb/article/view/227/166 |
| _version_ | 1828398798651523072 |
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| author | Motamedifar M Nozari A Azhdari Ghasrodashti E |
| author_facet | Motamedifar M Nozari A Azhdari Ghasrodashti E |
| author_sort | Motamedifar M |
| collection | DOAJ |
| description | Statement of Problem: Indiscriminate use of antibacterial agents increases the antibiotic resistance, which consequently necessitates seeking alternatives such as herbal remedies.
Objectives: The aim of this study was to evaluate the antimicrobial effect of hydroalcoholic extract of Helichrysum leucocephalum (H.leucocephalum) on Streptococcus mutans (S. mutans) growth as a major cause of dental caries.
Materials and Methods: In this study, hydroalcoholic extract of H. leucocephalum was prepared. The antibacterial effects, minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) of hydroalcoholic extract of H. leucocephalum and penicillin were assessed. Agar well diffusion method and micro broth dilution assay were used on bacterial suspension adjusted to a 0.5 McFarland standard (equivalent to 1.5×108 CFU/mL).Each test was repeated four times to minimize lab errors.
Results: In this study, the inhibitory zone of hydroalcoholic extract of H.leucocephalum in concentration of 100 mg/mL was 34±0.1 mm and for penicillin was 10 mm. The MIC and MBC of H.leucocephalum hydroalcoholic extract were 5.6±6.25, 21.6±6.25 mg/mL, respectively.
Conclusions: Hydroalcoholic extract of H. leucocephalum have antibacterial effect on S.mutans at a concentration of 12.5mg/mL, indicating possible application of this extract in prevention of dental caries; however, future researches are necessary. |
| first_indexed | 2024-12-10T09:07:16Z |
| format | Article |
| id | doaj.art-166d5b220f4645a192ef2005d15fea6e |
| institution | Directory Open Access Journal |
| issn | 2383-3971 2383-398X |
| language | English |
| last_indexed | 2024-12-10T09:07:16Z |
| publishDate | 2017-12-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Dental Biomaterial |
| spelling | doaj.art-166d5b220f4645a192ef2005d15fea6e2022-12-22T01:55:06ZengShiraz University of Medical SciencesJournal of Dental Biomaterial2383-39712383-398X2017-12-0144484488In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans GrowthMotamedifar M0Nozari A1 Azhdari Ghasrodashti E2Department of Bacteriology, Shiraz Medical School & Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranStatement of Problem: Indiscriminate use of antibacterial agents increases the antibiotic resistance, which consequently necessitates seeking alternatives such as herbal remedies. Objectives: The aim of this study was to evaluate the antimicrobial effect of hydroalcoholic extract of Helichrysum leucocephalum (H.leucocephalum) on Streptococcus mutans (S. mutans) growth as a major cause of dental caries. Materials and Methods: In this study, hydroalcoholic extract of H. leucocephalum was prepared. The antibacterial effects, minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) of hydroalcoholic extract of H. leucocephalum and penicillin were assessed. Agar well diffusion method and micro broth dilution assay were used on bacterial suspension adjusted to a 0.5 McFarland standard (equivalent to 1.5×108 CFU/mL).Each test was repeated four times to minimize lab errors. Results: In this study, the inhibitory zone of hydroalcoholic extract of H.leucocephalum in concentration of 100 mg/mL was 34±0.1 mm and for penicillin was 10 mm. The MIC and MBC of H.leucocephalum hydroalcoholic extract were 5.6±6.25, 21.6±6.25 mg/mL, respectively. Conclusions: Hydroalcoholic extract of H. leucocephalum have antibacterial effect on S.mutans at a concentration of 12.5mg/mL, indicating possible application of this extract in prevention of dental caries; however, future researches are necessary.http://jdb.sums.ac.ir/index.php/jdb/article/view/227/166AntimicrobialHelichrysum leucocephalumStreptococcus mutans |
| spellingShingle | Motamedifar M Nozari A Azhdari Ghasrodashti E In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth Journal of Dental Biomaterial Antimicrobial Helichrysum leucocephalum Streptococcus mutans |
| title | In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth |
| title_full | In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth |
| title_fullStr | In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth |
| title_full_unstemmed | In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth |
| title_short | In vitro Investigation of Hydro-alcoholic Extract of Helichrysum leucocephalum on the Inhibition of Streptococcus Mutans Growth |
| title_sort | in vitro investigation of hydro alcoholic extract of helichrysum leucocephalum on the inhibition of streptococcus mutans growth |
| topic | Antimicrobial Helichrysum leucocephalum Streptococcus mutans |
| url | http://jdb.sums.ac.ir/index.php/jdb/article/view/227/166 |
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