Modified Suture Technique in a Mouse Heart Transplant Model
The mouse abdominal heart transplantation model is a basic and important immunological research model. We developed a technique for placing entire everting sutures instead of half inverting and half everting sutures for anastomosis between donor and recipients' caval veins. The purpose of this...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-04-01
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| Series: | Asian Journal of Surgery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1015958411600250 |
| _version_ | 1811256066775711744 |
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| author | Song Su Tobias R. Türk Shengli Wu Hua Fan Jian Fu Kun Wu Ulrich Flögel Zhaoping Ding Andreas Kribben Oliver Witzke |
| author_facet | Song Su Tobias R. Türk Shengli Wu Hua Fan Jian Fu Kun Wu Ulrich Flögel Zhaoping Ding Andreas Kribben Oliver Witzke |
| author_sort | Song Su |
| collection | DOAJ |
| description | The mouse abdominal heart transplantation model is a basic and important immunological research model. We developed a technique for placing entire everting sutures instead of half inverting and half everting sutures for anastomosis between donor and recipients' caval veins. The purpose of this study was to evaluate this modified method.
Methods: Each technique was used in 25 mice subjected to isogenic abdominal heart transplantation. Recipient operation time, graft warm ischaemia time, time of caval anastomosis, and re-beating time were recorded. After transplantation, the heartbeat was palpated through the abdominal wall once a day for 100 days.
Results: Recipient operation time (40.7 ± 2.5 min vs. 44.3 ± 2.3 min, p < 0.01), cava-caval anastomosis time (8.4 ± 1.3 min vs. 12.1 ± 1.2 min, p < 0.01), and warm ischaemia time were significantly shorter (23.4 ± 1.7 min vs. 27.2 ± 1.6 min, p < 0.01) with the modified technique. Re-beating time was 1.2 ± 0.4 minutes with the modified technique vs. 1.5 ± 0.5 minutes (p = 0.04). There was a tendency for less surgical complications in the modified group, but there were no differences in survival rates.
Conclusion: The new suturing technique for mouse cardiac transplantation facilitates easier anastomosis of the outflow tract, thereby reducing operation, warm ischaemia, and re-beating times. |
| first_indexed | 2024-04-12T17:34:30Z |
| format | Article |
| id | doaj.art-166da9ae6384412691872657c072e023 |
| institution | Directory Open Access Journal |
| issn | 1015-9584 |
| language | English |
| last_indexed | 2024-04-12T17:34:30Z |
| publishDate | 2011-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Surgery |
| spelling | doaj.art-166da9ae6384412691872657c072e0232022-12-22T03:23:01ZengElsevierAsian Journal of Surgery1015-95842011-04-01342869110.1016/S1015-9584(11)60025-0Modified Suture Technique in a Mouse Heart Transplant ModelSong Su0Tobias R. Türk1Shengli Wu2Hua Fan3Jian Fu4Kun Wu5Ulrich Flögel6Zhaoping Ding7Andreas Kribben8Oliver Witzke9Department of Nephrology, University Hospital Essen, University of Duisburg-Essen, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, GermanyInstitute for Cellular Therapeutics, University of Louisville, USADepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, GermanyInstitute for Heart and Circulatory Physiology, Heinrich Heine University Düsseldorf, GermanyInstitute for Heart and Circulatory Physiology, Heinrich Heine University Düsseldorf, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, GermanyDepartment of Nephrology, University Hospital Essen, University of Duisburg-Essen, GermanyThe mouse abdominal heart transplantation model is a basic and important immunological research model. We developed a technique for placing entire everting sutures instead of half inverting and half everting sutures for anastomosis between donor and recipients' caval veins. The purpose of this study was to evaluate this modified method. Methods: Each technique was used in 25 mice subjected to isogenic abdominal heart transplantation. Recipient operation time, graft warm ischaemia time, time of caval anastomosis, and re-beating time were recorded. After transplantation, the heartbeat was palpated through the abdominal wall once a day for 100 days. Results: Recipient operation time (40.7 ± 2.5 min vs. 44.3 ± 2.3 min, p < 0.01), cava-caval anastomosis time (8.4 ± 1.3 min vs. 12.1 ± 1.2 min, p < 0.01), and warm ischaemia time were significantly shorter (23.4 ± 1.7 min vs. 27.2 ± 1.6 min, p < 0.01) with the modified technique. Re-beating time was 1.2 ± 0.4 minutes with the modified technique vs. 1.5 ± 0.5 minutes (p = 0.04). There was a tendency for less surgical complications in the modified group, but there were no differences in survival rates. Conclusion: The new suturing technique for mouse cardiac transplantation facilitates easier anastomosis of the outflow tract, thereby reducing operation, warm ischaemia, and re-beating times.http://www.sciencedirect.com/science/article/pii/S1015958411600250heart transplantation modelheterotopic transplantmouse |
| spellingShingle | Song Su Tobias R. Türk Shengli Wu Hua Fan Jian Fu Kun Wu Ulrich Flögel Zhaoping Ding Andreas Kribben Oliver Witzke Modified Suture Technique in a Mouse Heart Transplant Model Asian Journal of Surgery heart transplantation model heterotopic transplant mouse |
| title | Modified Suture Technique in a Mouse Heart Transplant Model |
| title_full | Modified Suture Technique in a Mouse Heart Transplant Model |
| title_fullStr | Modified Suture Technique in a Mouse Heart Transplant Model |
| title_full_unstemmed | Modified Suture Technique in a Mouse Heart Transplant Model |
| title_short | Modified Suture Technique in a Mouse Heart Transplant Model |
| title_sort | modified suture technique in a mouse heart transplant model |
| topic | heart transplantation model heterotopic transplant mouse |
| url | http://www.sciencedirect.com/science/article/pii/S1015958411600250 |
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