Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater
The use of the halophile microorganism Haloferax mediterranei, able to synthesize poly(hydroxybutyrate-hydroxyvalerate) (PHBV), is considered as a promising tool for the industrial production of bioplastic through bioprocessing. A consistent supplementation of the growth substrate in carbohydrates a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1000962/full |
| _version_ | 1811272003712188416 |
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| author | Marco Montemurro Gaia Salvatori Sara Alfano Andrea Martinelli Michela Verni Erica Pontonio Marianna Villano Marianna Villano Carlo Giuseppe Rizzello |
| author_facet | Marco Montemurro Gaia Salvatori Sara Alfano Andrea Martinelli Michela Verni Erica Pontonio Marianna Villano Marianna Villano Carlo Giuseppe Rizzello |
| author_sort | Marco Montemurro |
| collection | DOAJ |
| description | The use of the halophile microorganism Haloferax mediterranei, able to synthesize poly(hydroxybutyrate-hydroxyvalerate) (PHBV), is considered as a promising tool for the industrial production of bioplastic through bioprocessing. A consistent supplementation of the growth substrate in carbohydrates and minerals is overall necessary to allow its PHBV production. In this work, wasted bread was used as substrate for bioplastic production by microbial fermentation. Instead of the consistent and expensive minerals supplement required for Hfx. mediterranei DSM1411 growth, microfiltered seawater was added to the wasted bread-derived substrate. The suitable ratio of wasted bread homogenate and seawater, corresponding to 40:60, was selected. The addition of proteases and amylase to the bread homogenate promoted the microbial growth but it did not correspond to the increase of bioplastic production by the microorganism, that reach, under the experimental conditions, 1.53 g/L. An extraction procedure of the PHBV from cells, based on repeated washing with water, followed or not by a purification through ethanol precipitation, was applied instead of the conventional extraction with chloroform. Yield of PHBV obtained using the different extraction methods were 21.6 ± 3.6 (standard extraction/purification procedure with CHCl3:H2O mixture), 24.8 ± 3.0 (water-based extraction), and 19.8 ± 3.3 mg PHAs/g of wasted bread (water-based extraction followed by ethanol purification). Slightly higher hydroxyvalerate content (12.95 vs 10.78%, w/w) was found in PHBV obtained through the water-based extraction compared to the conventional one, moreover, the former was characterized by purity of 100% (w/w). Results demonstrated the suitability of wasted bread, supplemented with seawater, to be used as substrate for bioplastic production through fermentation. Results moreover demonstrated that a solvent-free extraction, exclusively based on osmotic shock, could be used to recover the bioplastic from cells. |
| first_indexed | 2024-04-12T22:32:25Z |
| format | Article |
| id | doaj.art-16706a47751d4cbb9eaa3722c40edac5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T22:32:25Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-16706a47751d4cbb9eaa3722c40edac52022-12-22T03:13:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.10009621000962Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawaterMarco Montemurro0Gaia Salvatori1Sara Alfano2Andrea Martinelli3Michela Verni4Erica Pontonio5Marianna Villano6Marianna Villano7Carlo Giuseppe Rizzello8Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, ItalyDepartment of Chemistry, Sapienza University of Rome, Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, Rome, ItalyDepartment of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, ItalyDepartment of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, ItalyDepartment of Chemistry, Sapienza University of Rome, Rome, ItalyResearch Center for Applied Sciences to the Safeguard of Environment and Cultural Heritage (CIABC), Sapienza University of Rome, Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, Rome, ItalyThe use of the halophile microorganism Haloferax mediterranei, able to synthesize poly(hydroxybutyrate-hydroxyvalerate) (PHBV), is considered as a promising tool for the industrial production of bioplastic through bioprocessing. A consistent supplementation of the growth substrate in carbohydrates and minerals is overall necessary to allow its PHBV production. In this work, wasted bread was used as substrate for bioplastic production by microbial fermentation. Instead of the consistent and expensive minerals supplement required for Hfx. mediterranei DSM1411 growth, microfiltered seawater was added to the wasted bread-derived substrate. The suitable ratio of wasted bread homogenate and seawater, corresponding to 40:60, was selected. The addition of proteases and amylase to the bread homogenate promoted the microbial growth but it did not correspond to the increase of bioplastic production by the microorganism, that reach, under the experimental conditions, 1.53 g/L. An extraction procedure of the PHBV from cells, based on repeated washing with water, followed or not by a purification through ethanol precipitation, was applied instead of the conventional extraction with chloroform. Yield of PHBV obtained using the different extraction methods were 21.6 ± 3.6 (standard extraction/purification procedure with CHCl3:H2O mixture), 24.8 ± 3.0 (water-based extraction), and 19.8 ± 3.3 mg PHAs/g of wasted bread (water-based extraction followed by ethanol purification). Slightly higher hydroxyvalerate content (12.95 vs 10.78%, w/w) was found in PHBV obtained through the water-based extraction compared to the conventional one, moreover, the former was characterized by purity of 100% (w/w). Results demonstrated the suitability of wasted bread, supplemented with seawater, to be used as substrate for bioplastic production through fermentation. Results moreover demonstrated that a solvent-free extraction, exclusively based on osmotic shock, could be used to recover the bioplastic from cells.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1000962/fullbioplasticHaloferax mediterraneiwasted breadbioprocessingseawaterpolyhydroxyalkanoates (PHA) |
| spellingShingle | Marco Montemurro Gaia Salvatori Sara Alfano Andrea Martinelli Michela Verni Erica Pontonio Marianna Villano Marianna Villano Carlo Giuseppe Rizzello Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater Frontiers in Microbiology bioplastic Haloferax mediterranei wasted bread bioprocessing seawater polyhydroxyalkanoates (PHA) |
| title | Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater |
| title_full | Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater |
| title_fullStr | Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater |
| title_full_unstemmed | Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater |
| title_short | Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater |
| title_sort | exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of haloferax mediterranei and seawater |
| topic | bioplastic Haloferax mediterranei wasted bread bioprocessing seawater polyhydroxyalkanoates (PHA) |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1000962/full |
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