Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts.
Vesicular lipoproteins (e.g., lipoprotein-X) are found in plasma in cholestasis or following infusion of Intralipid or phospholipid. To investigate the metabolism of vesicular lipoproteins, we isolated them from the plasma of subjects with cholestasis or following chronic or single Intralipid infusi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1988-10-01
|
| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520387836 |
| _version_ | 1818741417201106944 |
|---|---|
| author | K J Williams A R Tall I Tabas C Blum |
| author_facet | K J Williams A R Tall I Tabas C Blum |
| author_sort | K J Williams |
| collection | DOAJ |
| description | Vesicular lipoproteins (e.g., lipoprotein-X) are found in plasma in cholestasis or following infusion of Intralipid or phospholipid. To investigate the metabolism of vesicular lipoproteins, we isolated them from the plasma of subjects with cholestasis or following chronic or single Intralipid infusion. Cholestasis and chronic Intralipid therapy were found to be associated with elevated plasma concentrations of apoE, as determined by radioimmunoassay. Vesicular lipoproteins purified from each of the three types of plasma contained apoE, as well as other proteins. In cholestasis, in which levels of apoE were up to five times normal, a major portion of the plasma apoE was on vesicular lipoproteins. Normalized for apoE content, all preparations of vesicular lipoproteins displaced 125I-labeled LDL from apoB,E receptors of cultured fibroblasts identically. This displacement was inhibited by monoclonal antibodies that block receptor binding of apoE. Vesicular lipoproteins containing 125I-labeled apoE were internalized and degraded by fibroblasts. Different preparations caused small losses or gains of cellular cholesterol, with appropriate stimulation or suppression of apoB,E receptors. Thus, vesicular lipoproteins contain apoE, and apoE mediates their interaction with the apoB,E receptor. Our results suggest that the catabolism of cholesterol-rich vesicular lipoproteins, formed during cholestasis or following infusions of Intralipid or phospholipid, may be receptor-mediated. |
| first_indexed | 2024-12-18T01:56:17Z |
| format | Article |
| id | doaj.art-167316368ddf48d49a555e1a91b2184f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T01:56:17Z |
| publishDate | 1988-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-167316368ddf48d49a555e1a91b2184f2022-12-21T21:24:54ZengElsevierJournal of Lipid Research0022-22751988-10-01278892900Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts.K J WilliamsA R TallI TabasC BlumVesicular lipoproteins (e.g., lipoprotein-X) are found in plasma in cholestasis or following infusion of Intralipid or phospholipid. To investigate the metabolism of vesicular lipoproteins, we isolated them from the plasma of subjects with cholestasis or following chronic or single Intralipid infusion. Cholestasis and chronic Intralipid therapy were found to be associated with elevated plasma concentrations of apoE, as determined by radioimmunoassay. Vesicular lipoproteins purified from each of the three types of plasma contained apoE, as well as other proteins. In cholestasis, in which levels of apoE were up to five times normal, a major portion of the plasma apoE was on vesicular lipoproteins. Normalized for apoE content, all preparations of vesicular lipoproteins displaced 125I-labeled LDL from apoB,E receptors of cultured fibroblasts identically. This displacement was inhibited by monoclonal antibodies that block receptor binding of apoE. Vesicular lipoproteins containing 125I-labeled apoE were internalized and degraded by fibroblasts. Different preparations caused small losses or gains of cellular cholesterol, with appropriate stimulation or suppression of apoB,E receptors. Thus, vesicular lipoproteins contain apoE, and apoE mediates their interaction with the apoB,E receptor. Our results suggest that the catabolism of cholesterol-rich vesicular lipoproteins, formed during cholestasis or following infusions of Intralipid or phospholipid, may be receptor-mediated.http://www.sciencedirect.com/science/article/pii/S0022227520387836 |
| spellingShingle | K J Williams A R Tall I Tabas C Blum Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts. Journal of Lipid Research |
| title | Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts. |
| title_full | Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts. |
| title_fullStr | Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts. |
| title_full_unstemmed | Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts. |
| title_short | Recognition of vesicular lipoproteins by the apolipoprotein B,E receptor of cultured fibroblasts. |
| title_sort | recognition of vesicular lipoproteins by the apolipoprotein b e receptor of cultured fibroblasts |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520387836 |
| work_keys_str_mv | AT kjwilliams recognitionofvesicularlipoproteinsbytheapolipoproteinbereceptorofculturedfibroblasts AT artall recognitionofvesicularlipoproteinsbytheapolipoproteinbereceptorofculturedfibroblasts AT itabas recognitionofvesicularlipoproteinsbytheapolipoproteinbereceptorofculturedfibroblasts AT cblum recognitionofvesicularlipoproteinsbytheapolipoproteinbereceptorofculturedfibroblasts |