Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic Products
The <i>Varroa destructor</i> mite infests <i>Apis mellifera</i> colonies and causes significant harm. Traditional treatments have become less effective because of mite resistance development and can also generate residues inside beehives. This study aimed to gauge the efficac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/15/1/67 |
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| author | Eduardo José García-Vicente María Benito-Murcia María Martín Ismael Rey-Casero Ana Pérez María González Juan Manuel Alonso David Risco |
| author_facet | Eduardo José García-Vicente María Benito-Murcia María Martín Ismael Rey-Casero Ana Pérez María González Juan Manuel Alonso David Risco |
| author_sort | Eduardo José García-Vicente |
| collection | DOAJ |
| description | The <i>Varroa destructor</i> mite infests <i>Apis mellifera</i> colonies and causes significant harm. Traditional treatments have become less effective because of mite resistance development and can also generate residues inside beehives. This study aimed to gauge the efficacy of a beehive-derived postbiotic in reducing <i>V. destructor</i> viability and to explore its synergies with organic compounds. Four lactic acid bacteria (LAB) species, <i>Leuconostoc mesenteroides</i>, <i>Lactobacillus helsingborgensis</i>, <i>Bacillus velezensis</i>, and <i>Apilactobacillus kunkeei</i>, were isolated and tested in a postbiotic form (preparations of inanimate microorganisms and/or their components) via bioassays. <i>L. mesenteroides</i>, <i>L. helsingborgensis</i>, and <i>B. velezensis</i> notably reduced the mite viability compared to the control, and they were further tested together as a single postbiotic product (POS). Further bioassays were performed to assess the impact of the POS and its combinations with oxalic acid and oregano essential oil. The simple products and combinations (POS/Oregano, POS/Oxalic, Oregano/Oxalic, and POS/Oregano/Oxalic) decreased the mite viability. The most effective were the oxalic acid combinations (POS/Oregano/Oxalic, Oxalic/Oregano, POS/Oxalic), showing significant improvements compared to the individual products. These findings highlight the potential of combining organic products as a vital strategy for controlling <i>V. destructor</i> infection. This study suggests that these combinations could serve as essential tools for combating the impact of mites on bee colonies. |
| first_indexed | 2024-03-08T10:45:57Z |
| format | Article |
| id | doaj.art-1678a26ad06441988209bee158580bcc |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-08T10:45:57Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-1678a26ad06441988209bee158580bcc2024-01-26T17:04:18ZengMDPI AGInsects2075-44502024-01-011516710.3390/insects15010067Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic ProductsEduardo José García-Vicente0María Benito-Murcia1María Martín2Ismael Rey-Casero3Ana Pérez4María González5Juan Manuel Alonso6David Risco7Neobéitar S.L., Av. de Alemania, 6 1ºB, 10003 Cáceres, SpainNeobéitar S.L., Av. de Alemania, 6 1ºB, 10003 Cáceres, SpainNeobéitar S.L., Av. de Alemania, 6 1ºB, 10003 Cáceres, SpainNeobéitar S.L., Av. de Alemania, 6 1ºB, 10003 Cáceres, SpainNeobéitar S.L., Av. de Alemania, 6 1ºB, 10003 Cáceres, SpainNeobéitar S.L., Av. de Alemania, 6 1ºB, 10003 Cáceres, SpainDepartment of Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Av. de la Universidad s/n, 10001 Cáceres, SpainDepartment of Animal Medicine, Facultad de Veterinaria, Universidad de Extremadura, Av. de la Universidad s/n, 10001 Cáceres, SpainThe <i>Varroa destructor</i> mite infests <i>Apis mellifera</i> colonies and causes significant harm. Traditional treatments have become less effective because of mite resistance development and can also generate residues inside beehives. This study aimed to gauge the efficacy of a beehive-derived postbiotic in reducing <i>V. destructor</i> viability and to explore its synergies with organic compounds. Four lactic acid bacteria (LAB) species, <i>Leuconostoc mesenteroides</i>, <i>Lactobacillus helsingborgensis</i>, <i>Bacillus velezensis</i>, and <i>Apilactobacillus kunkeei</i>, were isolated and tested in a postbiotic form (preparations of inanimate microorganisms and/or their components) via bioassays. <i>L. mesenteroides</i>, <i>L. helsingborgensis</i>, and <i>B. velezensis</i> notably reduced the mite viability compared to the control, and they were further tested together as a single postbiotic product (POS). Further bioassays were performed to assess the impact of the POS and its combinations with oxalic acid and oregano essential oil. The simple products and combinations (POS/Oregano, POS/Oxalic, Oregano/Oxalic, and POS/Oregano/Oxalic) decreased the mite viability. The most effective were the oxalic acid combinations (POS/Oregano/Oxalic, Oxalic/Oregano, POS/Oxalic), showing significant improvements compared to the individual products. These findings highlight the potential of combining organic products as a vital strategy for controlling <i>V. destructor</i> infection. This study suggests that these combinations could serve as essential tools for combating the impact of mites on bee colonies.https://www.mdpi.com/2075-4450/15/1/67honey bee<i>Varroa destructor</i>lactic acid bacteriapostbioticoxalic acidoregano essential oil |
| spellingShingle | Eduardo José García-Vicente María Benito-Murcia María Martín Ismael Rey-Casero Ana Pérez María González Juan Manuel Alonso David Risco Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic Products Insects honey bee <i>Varroa destructor</i> lactic acid bacteria postbiotic oxalic acid oregano essential oil |
| title | Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic Products |
| title_full | Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic Products |
| title_fullStr | Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic Products |
| title_full_unstemmed | Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic Products |
| title_short | Evaluation of the Potential Effect of Postbiotics Obtained from Honey Bees against <i>Varroa destructor</i> and Their Combination with Other Organic Products |
| title_sort | evaluation of the potential effect of postbiotics obtained from honey bees against i varroa destructor i and their combination with other organic products |
| topic | honey bee <i>Varroa destructor</i> lactic acid bacteria postbiotic oxalic acid oregano essential oil |
| url | https://www.mdpi.com/2075-4450/15/1/67 |
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