Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli
RadA (also known as 'Sms') is a highly conserved protein, found in almost all eubacteria and plants, with sequence similarity to the RecA strand exchange protein and a role in homologous recombination. We investigate here the biochemical properties of the E. coli RadA protein and several m...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-02-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/10807 |
| _version_ | 1811199237193465856 |
|---|---|
| author | Deani L Cooper Susan T Lovett |
| author_facet | Deani L Cooper Susan T Lovett |
| author_sort | Deani L Cooper |
| collection | DOAJ |
| description | RadA (also known as 'Sms') is a highly conserved protein, found in almost all eubacteria and plants, with sequence similarity to the RecA strand exchange protein and a role in homologous recombination. We investigate here the biochemical properties of the E. coli RadA protein and several mutant forms. RadA is a DNA-dependent ATPase, a DNA-binding protein and can stimulate the branch migration phase of RecA-mediated strand transfer reactions. RadA cannot mediate synaptic pairing between homologous DNA molecules but can drive branch migration to extend the region of heteroduplex DNA, even without RecA. Unlike other branch migration factors RecG and RuvAB, RadA stimulates branch migration within the context of the RecA filament, in the direction of RecA-mediated strand exchange. We propose that RadA-mediated branch migration aids recombination by allowing the 3’ invading strand to be incorporated into heteroduplex DNA and to be extended by DNA polymerases. |
| first_indexed | 2024-04-12T01:44:38Z |
| format | Article |
| id | doaj.art-167e8bcf6b9640dcaac557d26c9a7486 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:44:38Z |
| publishDate | 2016-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-167e8bcf6b9640dcaac557d26c9a74862022-12-22T03:53:06ZengeLife Sciences Publications LtdeLife2050-084X2016-02-01510.7554/eLife.10807Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coliDeani L Cooper0Susan T Lovett1https://orcid.org/0000-0003-2792-1857Department of Biology, Brandeis University, Waltham, United States; Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, United StatesDepartment of Biology, Brandeis University, Waltham, United States; Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, United StatesRadA (also known as 'Sms') is a highly conserved protein, found in almost all eubacteria and plants, with sequence similarity to the RecA strand exchange protein and a role in homologous recombination. We investigate here the biochemical properties of the E. coli RadA protein and several mutant forms. RadA is a DNA-dependent ATPase, a DNA-binding protein and can stimulate the branch migration phase of RecA-mediated strand transfer reactions. RadA cannot mediate synaptic pairing between homologous DNA molecules but can drive branch migration to extend the region of heteroduplex DNA, even without RecA. Unlike other branch migration factors RecG and RuvAB, RadA stimulates branch migration within the context of the RecA filament, in the direction of RecA-mediated strand exchange. We propose that RadA-mediated branch migration aids recombination by allowing the 3’ invading strand to be incorporated into heteroduplex DNA and to be extended by DNA polymerases.https://elifesciences.org/articles/10807DNA repairDNA damage responsedouble-strand break repairDNA mutagenesishomologous recombination |
| spellingShingle | Deani L Cooper Susan T Lovett Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli eLife DNA repair DNA damage response double-strand break repair DNA mutagenesis homologous recombination |
| title | Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli |
| title_full | Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli |
| title_fullStr | Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli |
| title_full_unstemmed | Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli |
| title_short | Recombinational branch migration by the RadA/Sms paralog of RecA in Escherichia coli |
| title_sort | recombinational branch migration by the rada sms paralog of reca in escherichia coli |
| topic | DNA repair DNA damage response double-strand break repair DNA mutagenesis homologous recombination |
| url | https://elifesciences.org/articles/10807 |
| work_keys_str_mv | AT deanilcooper recombinationalbranchmigrationbytheradasmsparalogofrecainescherichiacoli AT susantlovett recombinationalbranchmigrationbytheradasmsparalogofrecainescherichiacoli |