Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating Populations
Cucumber (<i>Cucumis sativus</i> L.) fruit size/shape (FS) is an important yield and quality trait that is quantitatively inherited. Many quantitative trait loci (QTLs) for fruit size/shape have been identified, but very few have been fine-mapped or cloned. In this study, through marker-...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13384 |
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| author | Yupeng Pan Birong Chen Lijun Qiao Feifan Chen Jianyu Zhao Zhihui Cheng Yiqun Weng |
| author_facet | Yupeng Pan Birong Chen Lijun Qiao Feifan Chen Jianyu Zhao Zhihui Cheng Yiqun Weng |
| author_sort | Yupeng Pan |
| collection | DOAJ |
| description | Cucumber (<i>Cucumis sativus</i> L.) fruit size/shape (FS) is an important yield and quality trait that is quantitatively inherited. Many quantitative trait loci (QTLs) for fruit size/shape have been identified, but very few have been fine-mapped or cloned. In this study, through marker-assisted foreground and background selections, we developed near-isogenic lines (NILs) for a major-effect fruit size/shape QTL <i>FS5.2</i> in cucumber. Morphological and microscopic characterization of NILs suggests that the allele of <i>fs5.2</i> from the semi-wild Xishuangbanna (XIS) cucumber (<i>C</i>. <i>s</i>. var. <i>xishuangbannesis</i>) reduces fruit elongation but promotes radial growth resulting in shorter but wider fruit, which seems to be due to reduced cell length, but increased cellular layers. Consistent with this, the NIL carrying the homozygous XIS allele (<i>fs5.2</i>) had lower auxin/IAA contents in both the ovary and the developing fruit. Fine genetic mapping with NIL-derived segregating populations placed <i>FS5.2</i> into a 95.5 kb region with 15 predicted genes, and a homolog of the Arabidopsis <i>CRABS CLAW</i> (<i>CsCRC</i>) appeared to be the most possible candidate for <i>FS5.2</i>. Transcriptome profiling of NIL fruits at anthesis identified differentially expressed genes enriched in the auxin biosynthesis and signaling pathways, as well as genes involved in cell cycle, division, and cell wall processes. We conclude that the major-effect QTL <i>FS5.2</i> controls cucumber fruit size/shape through regulating auxin-mediated cell division and expansion for the lateral and longitudinal fruit growth, respectively. The gibberellic acid (GA) signaling pathway also plays a role in <i>FS5.2</i>-mediated fruit elongation. |
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| language | English |
| last_indexed | 2024-03-09T18:59:16Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-1680ca68eba441379d7cf85efa48e9662023-11-24T05:07:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211338410.3390/ijms232113384Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating PopulationsYupeng Pan0Birong Chen1Lijun Qiao2Feifan Chen3Jianyu Zhao4Zhihui Cheng5Yiqun Weng6Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USACollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaDepartment of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USADepartment of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USACollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaDepartment of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USACucumber (<i>Cucumis sativus</i> L.) fruit size/shape (FS) is an important yield and quality trait that is quantitatively inherited. Many quantitative trait loci (QTLs) for fruit size/shape have been identified, but very few have been fine-mapped or cloned. In this study, through marker-assisted foreground and background selections, we developed near-isogenic lines (NILs) for a major-effect fruit size/shape QTL <i>FS5.2</i> in cucumber. Morphological and microscopic characterization of NILs suggests that the allele of <i>fs5.2</i> from the semi-wild Xishuangbanna (XIS) cucumber (<i>C</i>. <i>s</i>. var. <i>xishuangbannesis</i>) reduces fruit elongation but promotes radial growth resulting in shorter but wider fruit, which seems to be due to reduced cell length, but increased cellular layers. Consistent with this, the NIL carrying the homozygous XIS allele (<i>fs5.2</i>) had lower auxin/IAA contents in both the ovary and the developing fruit. Fine genetic mapping with NIL-derived segregating populations placed <i>FS5.2</i> into a 95.5 kb region with 15 predicted genes, and a homolog of the Arabidopsis <i>CRABS CLAW</i> (<i>CsCRC</i>) appeared to be the most possible candidate for <i>FS5.2</i>. Transcriptome profiling of NIL fruits at anthesis identified differentially expressed genes enriched in the auxin biosynthesis and signaling pathways, as well as genes involved in cell cycle, division, and cell wall processes. We conclude that the major-effect QTL <i>FS5.2</i> controls cucumber fruit size/shape through regulating auxin-mediated cell division and expansion for the lateral and longitudinal fruit growth, respectively. The gibberellic acid (GA) signaling pathway also plays a role in <i>FS5.2</i>-mediated fruit elongation.https://www.mdpi.com/1422-0067/23/21/13384cucumberfruit shapeQTL cloningnear-isogenic linesmarker-assisted selectionauxin signaling |
| spellingShingle | Yupeng Pan Birong Chen Lijun Qiao Feifan Chen Jianyu Zhao Zhihui Cheng Yiqun Weng Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating Populations International Journal of Molecular Sciences cucumber fruit shape QTL cloning near-isogenic lines marker-assisted selection auxin signaling |
| title | Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating Populations |
| title_full | Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating Populations |
| title_fullStr | Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating Populations |
| title_full_unstemmed | Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating Populations |
| title_short | Phenotypic Characterization and Fine Mapping of a Major-Effect Fruit Shape QTL <i>FS5.2</i> in Cucumber, <i>Cucumis sativus</i> L., with Near-Isogenic Line-Derived Segregating Populations |
| title_sort | phenotypic characterization and fine mapping of a major effect fruit shape qtl i fs5 2 i in cucumber i cucumis sativus i l with near isogenic line derived segregating populations |
| topic | cucumber fruit shape QTL cloning near-isogenic lines marker-assisted selection auxin signaling |
| url | https://www.mdpi.com/1422-0067/23/21/13384 |
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