Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular Virotherapy
Oncolytic immunotherapy with competent viruses is an emerging approach in cancer treatment. The clinical safety of many types of oncolytic viruses (OVs) has been demonstrated. However, there is a lack of information about viral biodistribution in patients. The available data about oncolytic adenovir...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Molecular Therapy: Oncolytics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2372770520301236 |
| _version_ | 1818533817252577280 |
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| author | Ana Gómez David Sardón Teresa Cejalvo Fernando Vázquez Javier García-Castro Ana Judith Perisé-Barrios |
| author_facet | Ana Gómez David Sardón Teresa Cejalvo Fernando Vázquez Javier García-Castro Ana Judith Perisé-Barrios |
| author_sort | Ana Gómez |
| collection | DOAJ |
| description | Oncolytic immunotherapy with competent viruses is an emerging approach in cancer treatment. The clinical safety of many types of oncolytic viruses (OVs) has been demonstrated. However, there is a lack of information about viral biodistribution in patients. The available data about oncolytic adenovirus biodistribution in human subjects treated intravenously consists of virus detection in body fluids, a few tumor biopsies, and a single report of patient necropsy samples. There is no information about adenoviral biodistribution in patients treated intravenously with cellular vehicles carrying an oncolytic adenovirus. We previously published reports regarding the efficacy and clinical safety of infusing mesenchymal stem cells (MSCs) infected with an OV in human and canine patients. In this study, we performed necropsies on 12 canine patients treated with dCelyvir, canine MSCs infected with ICOCAV17, a canine oncolytic adenovirus. The prevalence of microscopic lesions, especially chronic inflammatory responses in different organs, was higher than expected. Concomitantly, we found a positive immunoreaction to ICOCAV17 in analyzed samples. These findings support a possible role of the virus in development of histopathological alterations and ongoing systemic viral replication of ICOCAV17 in the period after therapy administration. |
| first_indexed | 2024-12-11T18:03:45Z |
| format | Article |
| id | doaj.art-1681f94c6e594b6ba483873e131d460e |
| institution | Directory Open Access Journal |
| issn | 2372-7705 |
| language | English |
| last_indexed | 2024-12-11T18:03:45Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Oncolytics |
| spelling | doaj.art-1681f94c6e594b6ba483873e131d460e2022-12-22T00:55:49ZengElsevierMolecular Therapy: Oncolytics2372-77052020-09-0118525534Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular VirotherapyAna Gómez0David Sardón1Teresa Cejalvo2Fernando Vázquez3Javier García-Castro4Ana Judith Perisé-Barrios5Veterinary Pathology Unit, Universidad Alfonso X el Sabio, 28691 Madrid, SpainVeterinary Pathology Unit, Universidad Alfonso X el Sabio, 28691 Madrid, SpainCellular Biotechnology Unit, Instituto de Salud Carlos III, 28220 Madrid, SpainVeterinary Pathology Unit, Universidad Alfonso X el Sabio, 28691 Madrid, SpainCellular Biotechnology Unit, Instituto de Salud Carlos III, 28220 Madrid, Spain; Biomedical Research Unit, Universidad Alfonso X el Sabio, 28691 Madrid, SpainCellular Biotechnology Unit, Instituto de Salud Carlos III, 28220 Madrid, Spain; Biomedical Research Unit, Universidad Alfonso X el Sabio, 28691 Madrid, Spain; Corresponding author: Ana Judith Perisé Barrios, Biomedical Research Unit, Universidad Alfonso X El Sabio, 28691 Madrid, Spain.Oncolytic immunotherapy with competent viruses is an emerging approach in cancer treatment. The clinical safety of many types of oncolytic viruses (OVs) has been demonstrated. However, there is a lack of information about viral biodistribution in patients. The available data about oncolytic adenovirus biodistribution in human subjects treated intravenously consists of virus detection in body fluids, a few tumor biopsies, and a single report of patient necropsy samples. There is no information about adenoviral biodistribution in patients treated intravenously with cellular vehicles carrying an oncolytic adenovirus. We previously published reports regarding the efficacy and clinical safety of infusing mesenchymal stem cells (MSCs) infected with an OV in human and canine patients. In this study, we performed necropsies on 12 canine patients treated with dCelyvir, canine MSCs infected with ICOCAV17, a canine oncolytic adenovirus. The prevalence of microscopic lesions, especially chronic inflammatory responses in different organs, was higher than expected. Concomitantly, we found a positive immunoreaction to ICOCAV17 in analyzed samples. These findings support a possible role of the virus in development of histopathological alterations and ongoing systemic viral replication of ICOCAV17 in the period after therapy administration.http://www.sciencedirect.com/science/article/pii/S2372770520301236adenovirusoncolyticbiodistributionCelyvirimmunotherapyvirotherapy |
| spellingShingle | Ana Gómez David Sardón Teresa Cejalvo Fernando Vázquez Javier García-Castro Ana Judith Perisé-Barrios Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular Virotherapy Molecular Therapy: Oncolytics adenovirus oncolytic biodistribution Celyvir immunotherapy virotherapy |
| title | Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular Virotherapy |
| title_full | Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular Virotherapy |
| title_fullStr | Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular Virotherapy |
| title_full_unstemmed | Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular Virotherapy |
| title_short | Biodistribution Analysis of Oncolytic Adenoviruses in Canine Patient Necropsy Samples Treated with Cellular Virotherapy |
| title_sort | biodistribution analysis of oncolytic adenoviruses in canine patient necropsy samples treated with cellular virotherapy |
| topic | adenovirus oncolytic biodistribution Celyvir immunotherapy virotherapy |
| url | http://www.sciencedirect.com/science/article/pii/S2372770520301236 |
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