Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples
Background: Prolonged orthodontic treatment causes complications such as root resorption, gingivitis, and caries. Platelet-rich plasma (PRP) can accelerate the dental movements of orthodontic treatments. The aim of the present study was to evaluate the effect of local PRP injection on the secretion...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Dental Research Journal |
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| Online Access: | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2022;volume=19;issue=1;spage=68;epage=68;aulast=Hosseini |
| _version_ | 1818482854462488576 |
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| author | Seyed Mohammad Hosseini Shirin Zahra Farhad Nasim Esnaashari Esfahani Nafiseh Shirani |
| author_facet | Seyed Mohammad Hosseini Shirin Zahra Farhad Nasim Esnaashari Esfahani Nafiseh Shirani |
| author_sort | Seyed Mohammad Hosseini |
| collection | DOAJ |
| description | Background: Prolonged orthodontic treatment causes complications such as root resorption, gingivitis, and caries. Platelet-rich plasma (PRP) can accelerate the dental movements of orthodontic treatments. The aim of the present study was to evaluate the effect of local PRP injection on the secretion of dentin sialoprotein (DSP) and dentin matrix acidic phosphoprotein (DMP1) biomarkers in gingival crevicular fluid during orthodontic movements in animal samples.
Materials and Methods: In this experimental study, one maxillary quadrant of six beagle dogs was randomly selected as the experimental group, and the other side was considered as the control group. Afterward, maxillary of the first premolars was extracted, and then, a titanium nickel coil spring (150 g) was then used between the second premolar and the canine. PRP that was previously activated with CaCl 2-thrombin was injected intraligaman to the experimental side at days 0, 21, and 42. Moreover, the mixture of the CaCl 2-thrombin with placebo was injected in to the control side. The study period was 63 days. Sampling was performed on days 0, 1, 2, 7, 21, 42, and 63. Then, in all the samples, the DSP and DMP1 level was measured using special kits by ELISA method. Data were analyzed by the analysis of variance and t-test.
Results: Upon the application of orthodontic forces, the mean DMP1 and DSP levels in the experimental group at different times were significantly increased compared to the control group (P < 0.001).
Conclusion: PRP injection enhanced DMP1 and DSP level with probable rise in the rate of root resorption. |
| first_indexed | 2024-12-10T11:52:12Z |
| format | Article |
| id | doaj.art-16828c3fa26f4279843afb2df71484bb |
| institution | Directory Open Access Journal |
| issn | 1735-3327 2008-0255 |
| language | English |
| last_indexed | 2024-12-10T11:52:12Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Dental Research Journal |
| spelling | doaj.art-16828c3fa26f4279843afb2df71484bb2022-12-22T01:49:53ZengWolters Kluwer Medknow PublicationsDental Research Journal1735-33272008-02552022-01-01191686810.4103/1735-3327.353837Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samplesSeyed Mohammad HosseiniShirin Zahra FarhadNasim Esnaashari EsfahaniNafiseh ShiraniBackground: Prolonged orthodontic treatment causes complications such as root resorption, gingivitis, and caries. Platelet-rich plasma (PRP) can accelerate the dental movements of orthodontic treatments. The aim of the present study was to evaluate the effect of local PRP injection on the secretion of dentin sialoprotein (DSP) and dentin matrix acidic phosphoprotein (DMP1) biomarkers in gingival crevicular fluid during orthodontic movements in animal samples. Materials and Methods: In this experimental study, one maxillary quadrant of six beagle dogs was randomly selected as the experimental group, and the other side was considered as the control group. Afterward, maxillary of the first premolars was extracted, and then, a titanium nickel coil spring (150 g) was then used between the second premolar and the canine. PRP that was previously activated with CaCl 2-thrombin was injected intraligaman to the experimental side at days 0, 21, and 42. Moreover, the mixture of the CaCl 2-thrombin with placebo was injected in to the control side. The study period was 63 days. Sampling was performed on days 0, 1, 2, 7, 21, 42, and 63. Then, in all the samples, the DSP and DMP1 level was measured using special kits by ELISA method. Data were analyzed by the analysis of variance and t-test. Results: Upon the application of orthodontic forces, the mean DMP1 and DSP levels in the experimental group at different times were significantly increased compared to the control group (P < 0.001). Conclusion: PRP injection enhanced DMP1 and DSP level with probable rise in the rate of root resorption.http://www.drjjournal.net/article.asp?issn=1735-3327;year=2022;volume=19;issue=1;spage=68;epage=68;aulast=Hosseinidentin matrix proteindentin sialo proteinorthodontic treatmentplatelet-rich plasma |
| spellingShingle | Seyed Mohammad Hosseini Shirin Zahra Farhad Nasim Esnaashari Esfahani Nafiseh Shirani Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples Dental Research Journal dentin matrix protein dentin sialo protein orthodontic treatment platelet-rich plasma |
| title | Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples |
| title_full | Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples |
| title_fullStr | Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples |
| title_full_unstemmed | Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples |
| title_short | Effects of platelet-rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples |
| title_sort | effects of platelet rich plasma local injection on dentin sialo protein and dentin matrix acidic phosphoprotein 1 levels of secretion in gingival sulcular fluid during orthodontic movement in animal samples |
| topic | dentin matrix protein dentin sialo protein orthodontic treatment platelet-rich plasma |
| url | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2022;volume=19;issue=1;spage=68;epage=68;aulast=Hosseini |
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