Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity
The abusive use of antibiotics causes the destruction of intestinal flora and the proliferation of antibiotic-resistant pathogens. Date palm is used in traditional medicine in the Saharan regions due to its biological properties. The study aimed to identify the phytochemical composition and assess...
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2023-03-01
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| Series: | Foods and Raw Materials |
| Subjects: | |
| Online Access: | https://jfrm.ru/en/issues/20672/20792/ |
| _version_ | 1827971431165591552 |
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| author | Safia Ali Haimoud Rachida Allem |
| author_facet | Safia Ali Haimoud Rachida Allem |
| author_sort | Safia Ali Haimoud |
| collection | DOAJ |
| description | The abusive use of antibiotics causes the destruction of intestinal flora and the proliferation of antibiotic-resistant pathogens. Date palm is used in traditional medicine in the Saharan regions due to its biological properties.
The study aimed to identify the phytochemical composition and assess the antibacterial activity of the methanolic extracts of three date cultivars from Algeria. Their total phenolic, flavonoid, and flavonol contents were measured spectrophotometrically. The phytochemical screening was conducted by HPLC fingerprinting using twenty-three pure phenolic compounds as standards. The antibacterial activity against pathogenic bacterial species was assessed using the disk diffusion method.
The colorimetric methods showed that the total phenolic, flavonoid, and flavonol contents ranged from 2.13 ± 0.09 to 2.67 ± 0.02 mg GAE/100 g DW, 1.33 ± 0.21 to 1.55 ± 0.13 mg CEQ/100 g DW, and 0.41 ± 0.23 to 0.47 ± 0.05 mg REQ/100 g DW, respectively. HPLC fingerprinting showed that the extracts of date cultivars served as an excellent source of bioactive compounds (gallic acid, tannic acid, ferulic acid, vanillin, caffeine, quercetin, luteolin, rutin, aspegenin, isorhamnetin, and hesperidin). They also exhibited an antibacterial potential with an inhibition zone diameter ranging from 8.40 to 12.50 mm.
The results clearly demonstrate the antibacterial potency of date palm fruits, which could be attributed to their considerable content of phenolic compounds such as gallic acid, rutin, quercetin, and luteolin. |
| first_indexed | 2024-04-09T19:08:52Z |
| format | Article |
| id | doaj.art-1684a35a56474ceeae8690e638f9dead |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-04-09T19:08:52Z |
| publishDate | 2023-03-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-1684a35a56474ceeae8690e638f9dead2023-04-07T06:21:50ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992023-03-01111172410.21603/2308-4057-2023-1-549Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activitySafia Ali Haimoud0https://orcid.org/0000-0002-6693-7942Rachida Allem1https://orcid.org/0000-0003-2970-2543Hassiba Benbouali University of Chlef, Chlef, AlgeriaHassiba Benbouali University of Chlef, Chlef, AlgeriaThe abusive use of antibiotics causes the destruction of intestinal flora and the proliferation of antibiotic-resistant pathogens. Date palm is used in traditional medicine in the Saharan regions due to its biological properties. The study aimed to identify the phytochemical composition and assess the antibacterial activity of the methanolic extracts of three date cultivars from Algeria. Their total phenolic, flavonoid, and flavonol contents were measured spectrophotometrically. The phytochemical screening was conducted by HPLC fingerprinting using twenty-three pure phenolic compounds as standards. The antibacterial activity against pathogenic bacterial species was assessed using the disk diffusion method. The colorimetric methods showed that the total phenolic, flavonoid, and flavonol contents ranged from 2.13 ± 0.09 to 2.67 ± 0.02 mg GAE/100 g DW, 1.33 ± 0.21 to 1.55 ± 0.13 mg CEQ/100 g DW, and 0.41 ± 0.23 to 0.47 ± 0.05 mg REQ/100 g DW, respectively. HPLC fingerprinting showed that the extracts of date cultivars served as an excellent source of bioactive compounds (gallic acid, tannic acid, ferulic acid, vanillin, caffeine, quercetin, luteolin, rutin, aspegenin, isorhamnetin, and hesperidin). They also exhibited an antibacterial potential with an inhibition zone diameter ranging from 8.40 to 12.50 mm. The results clearly demonstrate the antibacterial potency of date palm fruits, which could be attributed to their considerable content of phenolic compounds such as gallic acid, rutin, quercetin, and luteolin.https://jfrm.ru/en/issues/20672/20792/phoenix dactylifera l.high-performance liquid chromatography fingerprintingphenolic compoundsflavonoidsflavonolsecondary metabolites |
| spellingShingle | Safia Ali Haimoud Rachida Allem Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity Foods and Raw Materials phoenix dactylifera l. high-performance liquid chromatography fingerprinting phenolic compounds flavonoids flavonol secondary metabolites |
| title | Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity |
| title_full | Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity |
| title_fullStr | Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity |
| title_full_unstemmed | Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity |
| title_short | Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity |
| title_sort | algerian date palm phoenix dactylifera l fruit cultivars hplc fingerprinting and antibacterial activity |
| topic | phoenix dactylifera l. high-performance liquid chromatography fingerprinting phenolic compounds flavonoids flavonol secondary metabolites |
| url | https://jfrm.ru/en/issues/20672/20792/ |
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