Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis
Abstract Background Cases of Mediterranean Spotted Fever like rickettsioses, caused by Rickettsia monacensis, have become more common in the last 10 years. In China, natural infection of R. monacensis in various tick species has been confirmed but the vector(s) of R. monacensis have not been recorde...
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| Format: | Article |
| Language: | English |
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BMC
2014-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-014-0512-8 |
| _version_ | 1797811663926198272 |
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| author | Xiaodong Ye Yi Sun Wendong Ju Xin Wang Wuchun Cao Mingyu Wu |
| author_facet | Xiaodong Ye Yi Sun Wendong Ju Xin Wang Wuchun Cao Mingyu Wu |
| author_sort | Xiaodong Ye |
| collection | DOAJ |
| description | Abstract Background Cases of Mediterranean Spotted Fever like rickettsioses, caused by Rickettsia monacensis, have become more common in the last 10 years. In China, natural infection of R. monacensis in various tick species has been confirmed but the vector(s) of R. monacensis have not been recorded. Methods The prevalence of R. monacensis in >1500 Ixodidae ticks from central and southern China was determined using centrifugation-shell vial culture and polymerase chain reaction techniques. The predominant species, Ixodes sinensis, harbored a natural infection of R. monacensis and was assumed to be a vector candidate of R. monacensis. Experimental transmissions were initialized by infecting Rickettsia-free tick colonies with R. monacensis using capillary tube feeding (CTF) or immersion techniques. Transstadial and transovarial transmissions, and transmission from ticks to mice, were conducted under laboratory conditions. Results R. monacensis was isolated and identified from hemolymph of Ixodes sinensis using molecular techniques. Transovarial transmission of R. monacensis from infected ♀I. sinensis to offspring was documented and infected offspring successfully passed Rickettsia to mice. Transstadial transmission rates were 58% in larva to nymph and 56% in nymph to adult stages. Infected nymphs and adults were also able to infect mice. Conclusions I. sinensis is a competence vector for R. monacensis as demonstrated by natural infection and transmission studies. |
| first_indexed | 2024-03-13T07:27:02Z |
| format | Article |
| id | doaj.art-1688780e558e4dd29ec8d8662e43368b |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:02Z |
| publishDate | 2014-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-1688780e558e4dd29ec8d8662e43368b2023-06-04T11:19:11ZengBMCParasites & Vectors1756-33052014-11-01711710.1186/s13071-014-0512-8Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensisXiaodong Ye0Yi Sun1Wendong Ju2Xin Wang3Wuchun Cao4Mingyu Wu5State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology & EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology & EpidemiologyCenter for Health Inspection, Heilongjiang Bureau of Entry & Exit Inspection and QuarantineCenter for Disease Control and Prevention of WenzhouState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology & EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology & EpidemiologyAbstract Background Cases of Mediterranean Spotted Fever like rickettsioses, caused by Rickettsia monacensis, have become more common in the last 10 years. In China, natural infection of R. monacensis in various tick species has been confirmed but the vector(s) of R. monacensis have not been recorded. Methods The prevalence of R. monacensis in >1500 Ixodidae ticks from central and southern China was determined using centrifugation-shell vial culture and polymerase chain reaction techniques. The predominant species, Ixodes sinensis, harbored a natural infection of R. monacensis and was assumed to be a vector candidate of R. monacensis. Experimental transmissions were initialized by infecting Rickettsia-free tick colonies with R. monacensis using capillary tube feeding (CTF) or immersion techniques. Transstadial and transovarial transmissions, and transmission from ticks to mice, were conducted under laboratory conditions. Results R. monacensis was isolated and identified from hemolymph of Ixodes sinensis using molecular techniques. Transovarial transmission of R. monacensis from infected ♀I. sinensis to offspring was documented and infected offspring successfully passed Rickettsia to mice. Transstadial transmission rates were 58% in larva to nymph and 56% in nymph to adult stages. Infected nymphs and adults were also able to infect mice. Conclusions I. sinensis is a competence vector for R. monacensis as demonstrated by natural infection and transmission studies.https://doi.org/10.1186/s13071-014-0512-8Rickettsia monacensisIxodes sinensisVector competence |
| spellingShingle | Xiaodong Ye Yi Sun Wendong Ju Xin Wang Wuchun Cao Mingyu Wu Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis Parasites & Vectors Rickettsia monacensis Ixodes sinensis Vector competence |
| title | Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis |
| title_full | Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis |
| title_fullStr | Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis |
| title_full_unstemmed | Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis |
| title_short | Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis |
| title_sort | vector competence of the tick ixodes sinensis acari ixodidae for rickettsia monacensis |
| topic | Rickettsia monacensis Ixodes sinensis Vector competence |
| url | https://doi.org/10.1186/s13071-014-0512-8 |
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