Tribology of enzymatically degraded cartilage mimicking early osteoarthritis
Abstract Healthy cartilage is a water-filled super lubricious tissue. Collagen type II provides it structural stability, and proteoglycans absorb water to keep the cartilage in a swollen condition, providing it the ability to creep and provide weeping lubrication. Osteoarthritis (OA) is a degenerati...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-05-01
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| Series: | Friction |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s40544-022-0701-y |
| _version_ | 1797784401123213312 |
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| author | Ke Ren Miguel Alejandro Reina Mahecha Maike Hübner Zhiwei Cui Hans J. Kaper Hugo C. van der Veen Prashant K. Sharma |
| author_facet | Ke Ren Miguel Alejandro Reina Mahecha Maike Hübner Zhiwei Cui Hans J. Kaper Hugo C. van der Veen Prashant K. Sharma |
| author_sort | Ke Ren |
| collection | DOAJ |
| description | Abstract Healthy cartilage is a water-filled super lubricious tissue. Collagen type II provides it structural stability, and proteoglycans absorb water to keep the cartilage in a swollen condition, providing it the ability to creep and provide weeping lubrication. Osteoarthritis (OA) is a degenerative and debilitating disorder of diarthrodial joints, where articular cartilage damage originates from enzymatic degradation and mechanical damage (wear). The objective of this research is to observe the level of cartilage damage present in knee arthroplasty patients and to understand the friction and creep behavior of enzymatically degraded bovine cartilage in vitro. Lateral (Lat) and medial (Med) condylar cartilages from OA patients undergoing total knee arthroplasty showed signs of enzymatic degradation and mechanical damage. Bovine cartilages were exposed to collagenase III and chondroitinase ABC to degrade collagen and proteoglycans, respectively. The loss of proteoglycans or collagen network and morphological changes were observed through histology and the atomic force microscope (AFM), respectively. A significant effect on creep due to enzymatic treatment was not observed. But the enzymatic treatment was found to significantly decrease the coefficient of friction (COF) at 4 N, while higher COF was shown from chondroitinase ABC degraded cartilage at 40 N. Collagenase III treatment leads to the release of intact proteoglycans at the sliding interface, while chondroitinase ABC treatment leads to the loss of chondroitin sulfate (CS) from the proteoglycans. Chondroitinase ABC-digested bovine cartilage mimicked patient samples the best because of the similar distributions of proteoglycans, collagen network, and friction behavior. |
| first_indexed | 2024-03-13T00:39:22Z |
| format | Article |
| id | doaj.art-16887ce186bd4660841838e1434caaa1 |
| institution | Directory Open Access Journal |
| issn | 2223-7690 2223-7704 |
| language | English |
| last_indexed | 2024-03-13T00:39:22Z |
| publishDate | 2023-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Friction |
| spelling | doaj.art-16887ce186bd4660841838e1434caaa12023-07-09T11:24:42ZengSpringerOpenFriction2223-76902223-77042023-05-011191724174010.1007/s40544-022-0701-yTribology of enzymatically degraded cartilage mimicking early osteoarthritisKe Ren0Miguel Alejandro Reina Mahecha1Maike Hübner2Zhiwei Cui3Hans J. Kaper4Hugo C. van der Veen5Prashant K. Sharma6Department of Biomedical Engineering, University Medical Centre Groningen, University of GroningenDepartment of Biomedical Engineering, University Medical Centre Groningen, University of GroningenDepartment of Biomedical Engineering, University Medical Centre Groningen, University of GroningenDepartment of Mechanical Engineering, Eindhoven University of TechnologyDepartment of Biomedical Engineering, University Medical Centre Groningen, University of GroningenDepartment of Orthopedic Surgery, University Medical Centre Groningen, University of GroningenDepartment of Biomedical Engineering, University Medical Centre Groningen, University of GroningenAbstract Healthy cartilage is a water-filled super lubricious tissue. Collagen type II provides it structural stability, and proteoglycans absorb water to keep the cartilage in a swollen condition, providing it the ability to creep and provide weeping lubrication. Osteoarthritis (OA) is a degenerative and debilitating disorder of diarthrodial joints, where articular cartilage damage originates from enzymatic degradation and mechanical damage (wear). The objective of this research is to observe the level of cartilage damage present in knee arthroplasty patients and to understand the friction and creep behavior of enzymatically degraded bovine cartilage in vitro. Lateral (Lat) and medial (Med) condylar cartilages from OA patients undergoing total knee arthroplasty showed signs of enzymatic degradation and mechanical damage. Bovine cartilages were exposed to collagenase III and chondroitinase ABC to degrade collagen and proteoglycans, respectively. The loss of proteoglycans or collagen network and morphological changes were observed through histology and the atomic force microscope (AFM), respectively. A significant effect on creep due to enzymatic treatment was not observed. But the enzymatic treatment was found to significantly decrease the coefficient of friction (COF) at 4 N, while higher COF was shown from chondroitinase ABC degraded cartilage at 40 N. Collagenase III treatment leads to the release of intact proteoglycans at the sliding interface, while chondroitinase ABC treatment leads to the loss of chondroitin sulfate (CS) from the proteoglycans. Chondroitinase ABC-digested bovine cartilage mimicked patient samples the best because of the similar distributions of proteoglycans, collagen network, and friction behavior.https://doi.org/10.1007/s40544-022-0701-ylubricationcartilageboundary lubricationosteoarthritis (OA) |
| spellingShingle | Ke Ren Miguel Alejandro Reina Mahecha Maike Hübner Zhiwei Cui Hans J. Kaper Hugo C. van der Veen Prashant K. Sharma Tribology of enzymatically degraded cartilage mimicking early osteoarthritis Friction lubrication cartilage boundary lubrication osteoarthritis (OA) |
| title | Tribology of enzymatically degraded cartilage mimicking early osteoarthritis |
| title_full | Tribology of enzymatically degraded cartilage mimicking early osteoarthritis |
| title_fullStr | Tribology of enzymatically degraded cartilage mimicking early osteoarthritis |
| title_full_unstemmed | Tribology of enzymatically degraded cartilage mimicking early osteoarthritis |
| title_short | Tribology of enzymatically degraded cartilage mimicking early osteoarthritis |
| title_sort | tribology of enzymatically degraded cartilage mimicking early osteoarthritis |
| topic | lubrication cartilage boundary lubrication osteoarthritis (OA) |
| url | https://doi.org/10.1007/s40544-022-0701-y |
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