Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites
Abstract Clathrin-mediated endocytosis plays an important role in the recycling of synaptic vesicle in presynaptic terminals, and in the recycling of transmitter receptors in neuronal soma/dendrites. The present study uses electron microscopy (EM) and immunogold EM to document the different categori...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | Molecular Brain |
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| Online Access: | http://link.springer.com/article/10.1186/s13041-020-00683-5 |
| _version_ | 1818403585610743808 |
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| author | Jung-Hwa Tao-Cheng |
| author_facet | Jung-Hwa Tao-Cheng |
| author_sort | Jung-Hwa Tao-Cheng |
| collection | DOAJ |
| description | Abstract Clathrin-mediated endocytosis plays an important role in the recycling of synaptic vesicle in presynaptic terminals, and in the recycling of transmitter receptors in neuronal soma/dendrites. The present study uses electron microscopy (EM) and immunogold EM to document the different categories of clathrin-coated vesicles (CCV) and pits (CCP) in axons compared to soma/dendrites, and the depolarization-induced redistribution of clathrin in these two polarized compartments of the neuron. The size of CCVs in presynaptic terminals (~ 40 nm; similar to the size of synaptic vesicles) is considerably smaller than the size of CCVs in soma/dendrites (~ 90 nm). Furthermore, neuronal stimulation induces an increase in the number of CCV/CCP in presynaptic terminals, but a decrease in soma/dendrites. Immunogold labeling of clathrin revealed that in presynaptic terminals under resting conditions, the majority of clathrin molecules are unassembled and concentrated outside of synaptic vesicle clusters. Upon depolarization with high K+, label for clathrin became scattered among de-clustered synaptic vesicles and moved closer to the presynaptic active zone. In contrast to axons, clathrin-labeled CCVs and CCPs were prominent in soma/dendrites under resting conditions, and became inconspicuous upon depolarization with high K+. Thus, EM examination suggests that the regulation and mechanism of clathrin-mediated endocytosis differ between axon and dendrite, and that clathrin redistributes differently in these two neuronal compartments upon depolarization. |
| first_indexed | 2024-12-14T08:26:36Z |
| format | Article |
| id | doaj.art-1688eccbf32b4b3c9fff4475f8f56661 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-14T08:26:36Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-1688eccbf32b4b3c9fff4475f8f566612022-12-21T23:09:38ZengBMCMolecular Brain1756-66062020-10-0113111110.1186/s13041-020-00683-5Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendritesJung-Hwa Tao-Cheng0NINDS Electron Microscopy Facility, National Institute of Neurological Disorders and Stroke, National Institutes of HealthAbstract Clathrin-mediated endocytosis plays an important role in the recycling of synaptic vesicle in presynaptic terminals, and in the recycling of transmitter receptors in neuronal soma/dendrites. The present study uses electron microscopy (EM) and immunogold EM to document the different categories of clathrin-coated vesicles (CCV) and pits (CCP) in axons compared to soma/dendrites, and the depolarization-induced redistribution of clathrin in these two polarized compartments of the neuron. The size of CCVs in presynaptic terminals (~ 40 nm; similar to the size of synaptic vesicles) is considerably smaller than the size of CCVs in soma/dendrites (~ 90 nm). Furthermore, neuronal stimulation induces an increase in the number of CCV/CCP in presynaptic terminals, but a decrease in soma/dendrites. Immunogold labeling of clathrin revealed that in presynaptic terminals under resting conditions, the majority of clathrin molecules are unassembled and concentrated outside of synaptic vesicle clusters. Upon depolarization with high K+, label for clathrin became scattered among de-clustered synaptic vesicles and moved closer to the presynaptic active zone. In contrast to axons, clathrin-labeled CCVs and CCPs were prominent in soma/dendrites under resting conditions, and became inconspicuous upon depolarization with high K+. Thus, EM examination suggests that the regulation and mechanism of clathrin-mediated endocytosis differ between axon and dendrite, and that clathrin redistributes differently in these two neuronal compartments upon depolarization.http://link.springer.com/article/10.1186/s13041-020-00683-5Electron microscopyClathrin-coated vesiclesClathrin-coated pitsEndocytosisSynaptic vesicleMultivesicular body |
| spellingShingle | Jung-Hwa Tao-Cheng Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites Molecular Brain Electron microscopy Clathrin-coated vesicles Clathrin-coated pits Endocytosis Synaptic vesicle Multivesicular body |
| title | Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites |
| title_full | Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites |
| title_fullStr | Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites |
| title_full_unstemmed | Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites |
| title_short | Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites |
| title_sort | stimulation induced differential redistributions of clathrin and clathrin coated vesicles in axons compared to soma dendrites |
| topic | Electron microscopy Clathrin-coated vesicles Clathrin-coated pits Endocytosis Synaptic vesicle Multivesicular body |
| url | http://link.springer.com/article/10.1186/s13041-020-00683-5 |
| work_keys_str_mv | AT junghwataocheng stimulationinduceddifferentialredistributionsofclathrinandclathrincoatedvesiclesinaxonscomparedtosomadendrites |